OCA at 10:55, 14 June 2023

2023-06-14T10:55:57Z

←Older revision		Revision as of 10:55, 14 June 2023
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	==NMR Structure of the DNA binding domain of EhMybS3==		==NMR Structure of the DNA binding domain of EhMybS3==
-	<StructureSection load='6nvz' size='340' side='right'caption='[[6nvz~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6nvz' size='340' side='right'caption='[[6nvz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nvz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NVZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NVZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nvz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NVZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nvz OCA], [~~http~~://pdbe.org/6nvz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nvz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nvz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nvz ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nvz OCA], [https://pdbe.org/6nvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nvz RCSB], [https://www.ebi.ac.uk/pdbsum/6nvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nvz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C4M2B1_ENTH1 C4M2B1_ENTH1]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amoebiasis is the third leading cause of death among protozoon parasitic diseases in the lower-middle income countries. Understanding the molecular events that control gene expression such as transcription factors, their DNA binding mode and target sequences can help to develop new antiamoebic drugs against Entamoeba histolytica. In this paper we performed a genome and structural analysis of a specific transcription factor. The genome of E. histolytica codifies for 9 EhMybSHAQKYF proteins, which are a family within a large group of 34 Myb-DNA-binding domain (Myb-DBD) containing proteins. Here we compared Entamoeba Myb-SHAQKYF proteins with Myb-like proteins from the Reveille (RVE) family, important regulators of plant circadian networks. This comparison could lead to stablish their role in E. histolytica life cycle. We show that the ehmybshaqkyf genes are differentially expressed in trophozoites under basal cell culture conditions. An in-silico analysis predicts that members of this group harbor a highly conserved and structured Myb-DBD and a large portion of intrinsically disordered residues. As the Myb-DBD of these proteins harbors a distinctive Q[VI]R[ST]HAQK[YF]F sequence in its putative third alpha-helix, we consider relevant to determine the three-dimensional (3D) structure of one of them. An NMR structure of the Myb-DBD of EhMybS3 shows that this protein is composed of three alpha-helices stabilized by a hydrophobic core, similar to Myb proteins of different kingdoms. It is remarkable that despite not sharing similarities in their amino acid sequences, the structure of the Myb-DBD of the EhMybS3 is well conserved in this early branching eukaryote.
		+
		+	Genome-wide and structural analysis of the Myb-SHAQKYF family in Entamoeba histolytica.,Cardenas-Hernandez H, Titaux-Delgado GA, Castaneda-Ortiz EJ, Torres-Larios A, Brieba LG, Del Rio-Portilla F, Azuara-Liceaga E Biochim Biophys Acta Proteins Proteom. 2021 Apr;1869(4):140601. doi: , 10.1016/j.bbapap.2021.140601. Epub 2021 Jan 7. PMID:33422669<ref>PMID:33422669</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6nvz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Entamoeba histolytica]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Rio-Portilla, f del]]~~	+	[[Category: Titaux-Delgado GA]]
-	[[Category: Titaux-Delgado~~, G A~~]]	+	[[Category: Del Rio-Portilla f]]
-	[[Category: ~~Dna]]~~	+
-	~~[[Category: Dna binding protein~~]]	+

OCA at 04:16, 13 February 2020

2020-02-13T04:16:29Z

←Older revision		Revision as of 04:16, 13 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6nvz is ~~ON HOLD until Paper Publication~~	+	==NMR Structure of the DNA binding domain of EhMybS3==
-		+	<StructureSection load='6nvz' size='340' side='right'caption='[[6nvz]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6nvz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NVZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nvz OCA], [http://pdbe.org/6nvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nvz RCSB], [http://www.ebi.ac.uk/pdbsum/6nvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nvz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Rio-Portilla, f del]]
		+	[[Category: Titaux-Delgado, G A]]
		+	[[Category: Dna]]
		+	[[Category: Dna binding protein]]

OCA at 04:55, 27 March 2019

2019-03-27T04:55:54Z

←Older revision		Revision as of 04:55, 27 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nvz is ON HOLD	+	The entry 6nvz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6nvz" [edit=sysop:move=sysop]

2019-02-13T07:51:01Z

Protected "6nvz" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:51, 13 February 2019

OCA: New page: '''Unreleased structure''' The entry 6nvz is ON HOLD Authors: Description: Category: Unreleased Structures

2019-02-13T07:51:00Z

New page: '''Unreleased structure''' The entry 6nvz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nvz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6nvz - Revision history

OCA at 10:55, 14 June 2023

OCA at 04:16, 13 February 2020

OCA at 04:55, 27 March 2019

OCA: Protected "6nvz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6nvz is ON HOLD Authors: Description: Category: Unreleased Structures