6nza - Revision history

OCA at 07:01, 11 October 2023

2023-10-11T07:01:53Z

←Older revision		Revision as of 07:01, 11 October 2023
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	<StructureSection load='6nza' size='340' side='right'caption='[[6nza]], [[Resolution\|resolution]] 1.41Å' scene=''>		<StructureSection load='6nza' size='340' side='right'caption='[[6nza]], [[Resolution\|resolution]] 1.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nza]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nza]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NZA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.406Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">fumC, b1611, JW1603 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nza OCA], [https://pdbe.org/6nza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nza RCSB], [https://www.ebi.ac.uk/pdbsum/6nza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nza ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nza OCA], [~~http~~://pdbe.org/6nza PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nza RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nza PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nza ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>	+	[https://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6nza" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6nza" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fumarase\|Fumarase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Fumarate hydratase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhattacharyya, B]]	+	[[Category: Bhattacharyya B]]
-	[[Category: May~~, J F~~]]	+	[[Category: May JF]]
-	[[Category: Weaver~~, T M]]~~	+	[[Category: Weaver TM]]
-	~~[[Category: Citrate]]~~	+
-	~~[[Category: Fumarase]]~~	+
-	~~[[Category: Krebs cycle]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Metabolism~~]]	+

OCA at 04:40, 13 February 2020

2020-02-13T04:40:13Z

←Older revision		Revision as of 04:40, 13 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6nza is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of E. coli fumarase C K324A variant with closed SS Loop at 1.41 angstrom resolution==
		+	<StructureSection load='6nza' size='340' side='right'caption='[[6nza]], [[Resolution\|resolution]] 1.41Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NZA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fumC, b1611, JW1603 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nza OCA], [http://pdbe.org/6nza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nza RCSB], [http://www.ebi.ac.uk/pdbsum/6nza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nza ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fumarase C (FumC) catalyzes the reversible conversion of fumarate to S-malate. Previous structural investigations within the superfamily have reported a dynamic structural segment, termed the SS Loop. To date, active-site asymmetry has raised the question of how SS Loop placement affects participation of key residues during the reaction. Herein, we report structural and kinetic analyses from Escherichia coli FumC variants to understand the contribution of SS Loop residues S318, K324, and N326. High-resolution X-ray crystallographic results reveal three distinct FumC active-site conformations; disordered-open, ordered-open, and the newly discovered ordered-closed. Surprisingly, each SS Loop variant has unaffected Michaelis constants coupled to reductions in turnover number. Based upon our structural and functional analyses, we propose structural and catalytic roles for each of the aforementioned residues.

-	~~Authors~~:	+	Closed fumarase C active-site structures reveal SS Loop residue contribution in catalysis.,Stuttgen GM, Grosskopf JD, Berger CR, May JF, Bhattacharyya B, Weaver TM FEBS Lett. 2020 Jan;594(2):337-357. doi: 10.1002/1873-3468.13603. Epub 2019 Sep, 23. PMID:31514245<ref>PMID:31514245</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6nza" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Fumarate hydratase]]
		+	[[Category: Large Structures]]
		+	[[Category: Bhattacharyya, B]]
		+	[[Category: May, J F]]
		+	[[Category: Weaver, T M]]
		+	[[Category: Citrate]]
		+	[[Category: Fumarase]]
		+	[[Category: Krebs cycle]]
		+	[[Category: Lyase]]
		+	[[Category: Metabolism]]

OCA at 04:32, 3 April 2019

2019-04-03T04:32:29Z

←Older revision		Revision as of 04:32, 3 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nza is ON HOLD	+	The entry 6nza is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6nza" [edit=sysop:move=sysop]

2019-02-21T05:15:51Z

Protected "6nza" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 21 February 2019

OCA: New page: '''Unreleased structure''' The entry 6nza is ON HOLD Authors: Description: Category: Unreleased Structures

2019-02-21T05:15:50Z

New page: '''Unreleased structure''' The entry 6nza is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6nza is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]