6nzb - Revision history

OCA at 07:01, 11 October 2023

2023-10-11T07:01:54Z

←Older revision		Revision as of 07:01, 11 October 2023
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	<StructureSection load='6nzb' size='340' side='right'caption='[[6nzb]], [[Resolution\|resolution]] 1.37Å' scene=''>		<StructureSection load='6nzb' size='340' side='right'caption='[[6nzb]], [[Resolution\|resolution]] 1.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6nzb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6NZB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6nzb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NZB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.37Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">fumC, b1611, JW1603 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=83333 ECOLI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nzb OCA], [https://pdbe.org/6nzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nzb RCSB], [https://www.ebi.ac.uk/pdbsum/6nzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nzb ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6nzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nzb OCA], [~~http~~://pdbe.org/6nzb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6nzb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6nzb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6nzb ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>	+	[https://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6nzb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6nzb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fumarase\|Fumarase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Fumarate hydratase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhattacharyya, B]]	+	[[Category: Bhattacharyya B]]
-	[[Category: May~~, J F~~]]	+	[[Category: May JF]]
-	[[Category: Weaver~~, T M]]~~	+	[[Category: Weaver TM]]
-	~~[[Category: Citrate]]~~	+
-	~~[[Category: Fumarase]]~~	+
-	~~[[Category: Krebs cycle]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Metabolism~~]]	+

OCA at 04:40, 13 February 2020

2020-02-13T04:40:18Z

←Older revision		Revision as of 04:40, 13 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6nzb is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of E. coli fumarase C S318A variant with closed SS Loop at 1.37 angstrom resolution==
		+	<StructureSection load='6nzb' size='340' side='right'caption='[[6nzb]], [[Resolution\|resolution]] 1.37Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6nzb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NZB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NZB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fumC, b1611, JW1603 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nzb OCA], [http://pdbe.org/6nzb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nzb RCSB], [http://www.ebi.ac.uk/pdbsum/6nzb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nzb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fumarase C (FumC) catalyzes the reversible conversion of fumarate to S-malate. Previous structural investigations within the superfamily have reported a dynamic structural segment, termed the SS Loop. To date, active-site asymmetry has raised the question of how SS Loop placement affects participation of key residues during the reaction. Herein, we report structural and kinetic analyses from Escherichia coli FumC variants to understand the contribution of SS Loop residues S318, K324, and N326. High-resolution X-ray crystallographic results reveal three distinct FumC active-site conformations; disordered-open, ordered-open, and the newly discovered ordered-closed. Surprisingly, each SS Loop variant has unaffected Michaelis constants coupled to reductions in turnover number. Based upon our structural and functional analyses, we propose structural and catalytic roles for each of the aforementioned residues.

-	~~Authors: Weaver, T.M~~., May, J.F., ~~Bhattacharyya, B~~.	+	Closed fumarase C active-site structures reveal SS Loop residue contribution in catalysis.,Stuttgen GM, Grosskopf JD, Berger CR, May JF, Bhattacharyya B, Weaver TM FEBS Lett. 2020 Jan;594(2):337-357. doi: 10.1002/1873-3468.13603. Epub 2019 Sep, 23. PMID:31514245<ref>PMID:31514245</ref>

-	~~Description: Crystal structure~~ of E. ~~coli fumarase C S318A variant with closed SS Loop at 1~~.~~37 angstrom resolution~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~May, J.F~~]]	+	<div class="pdbe-citations 6nzb" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Weaver, T.M~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ecoli]]
		+	[[Category: Fumarate hydratase]]
		+	[[Category: Large Structures]]
	[[Category: Bhattacharyya, B]]		[[Category: Bhattacharyya, B]]
		+	[[Category: May, J F]]
		+	[[Category: Weaver, T M]]
		+	[[Category: Citrate]]
		+	[[Category: Fumarase]]
		+	[[Category: Krebs cycle]]
		+	[[Category: Lyase]]
		+	[[Category: Metabolism]]

OCA at 04:32, 3 April 2019

2019-04-03T04:32:34Z

←Older revision		Revision as of 04:32, 3 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6nzb is ON HOLD	+	The entry 6nzb is ON HOLD until Paper Publication

	Authors: Weaver, T.M., May, J.F., Bhattacharyya, B.		Authors: Weaver, T.M., May, J.F., Bhattacharyya, B.

OCA at 05:55, 20 March 2019

2019-03-20T05:55:02Z

←Older revision		Revision as of 05:55, 20 March 2019
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	Authors: Weaver, T.M., May, J.F., Bhattacharyya, B.		Authors: Weaver, T.M., May, J.F., Bhattacharyya, B.

-	Description: Crystal structure of E. coli fumarase C ~~K324A~~ variant with closed SS Loop at 1.41 angstrom resolution	+	Description: Crystal structure of E. coli fumarase C S318A variant with closed SS Loop at 1.37 angstrom resolution
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: May, J.F]]		[[Category: May, J.F]]
	[[Category: Weaver, T.M]]		[[Category: Weaver, T.M]]
	[[Category: Bhattacharyya, B]]		[[Category: Bhattacharyya, B]]

OCA: Protected "6nzb" [edit=sysop:move=sysop]

2019-02-21T05:15:54Z

Protected "6nzb" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 21 February 2019

OCA: New page: '''Unreleased structure''' The entry 6nzb is ON HOLD Authors: Weaver, T.M., May, J.F., Bhattacharyya, B. Description: Crystal structure of E. coli fumarase C K324A variant with closed ...

2019-02-21T05:15:53Z

New page: '''Unreleased structure''' The entry 6nzb is ON HOLD Authors: Weaver, T.M., May, J.F., Bhattacharyya, B. Description: Crystal structure of E. coli fumarase C K324A variant with closed ...

New page

'''Unreleased structure'''

The entry 6nzb is ON HOLD

Authors: Weaver, T.M., May, J.F., Bhattacharyya, B.

Description: Crystal structure of E. coli fumarase C K324A variant with closed SS Loop at 1.41 angstrom resolution
[[Category: Unreleased Structures]]
[[Category: May, J.F]]
[[Category: Weaver, T.M]]
[[Category: Bhattacharyya, B]]