6o38 - Revision history

OCA at 07:01, 31 July 2019

2019-07-31T07:01:11Z

←Older revision		Revision as of 07:01, 31 July 2019
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	<StructureSection load='6o38' size='340' side='right'caption='[[6o38]], [[Resolution\|resolution]] 2.59Å' scene=''>		<StructureSection load='6o38' size='340' side='right'caption='[[6o38]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6o38]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O38 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6o38]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_baumannii_m2 Acinetobacter baumannii m2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O38 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o38 OCA], [http://pdbe.org/6o38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o38 RCSB], [http://www.ebi.ac.uk/pdbsum/6o38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o38 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o38 OCA], [http://pdbe.org/6o38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o38 RCSB], [http://www.ebi.ac.uk/pdbsum/6o38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o38 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Members of the Acinetobacter baumannii-calcoaceticus complex are nosocomial pathogens frequently causing multidrug-resistant infections that are increasing at alarming rates. A. baumannii has become the Gram-negative bacterium with the highest rate of multidrug resistance. As such, it is categorized by the World Health Organization as a critical priority for the research and development of new antimicrobial therapies. The zinc-dependent metallo-endopeptidase CpaA is a predominant substrate of the type II secretion system (T2SS). CpaA is also a virulence factor of medically relevant Acinetobacter strains that specifically degrade the human glycoprotein coagulation factor XII and not its deglycosylated form, but the mechanism for this specificity is unknown. CpaB is a membrane-anchored T2SS chaperone that interacts with CpaA and is required for its stability and secretion. Here, we report the crystal structure of the CpaAB complex at 2.6 A resolution, revealing four glycan-binding domains in CpaA that were not predicted from its primary sequence and may explain CpaA's glycoprotein-targeting activity. The structure of the complex identified a novel mode for chaperone-protease interactions in which the protease surrounds the chaperone. The CpaAB organization was akin to zymogen inactivation, with CpaB serving as a prodomain that inhibits catalytically active CpaA. CpaB contains a C-terminal tail that appears to block access to the CpaA catalytic site, and functional experiments with truncated variants indicated that this tail is dispensable for CpaA expression and secretion. Our results provide new insight into the mechanism of CpaA secretion and may inform the future development of therapeutic strategies for managing Acinetobacter infections.
		+
		+	The structure of Acinetobacter secreted protease CpaA complexed with its chaperone CpaB reveals a novel mode of a T2SS chaperone-substrate interaction.,Urusova DV, Kinsella RL, Salinas ND, Haurat MF, Feldman MF, Tolia NH J Biol Chem. 2019 Jul 18. pii: RA119.009805. doi: 10.1074/jbc.RA119.009805. PMID:31320476<ref>PMID:31320476</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6o38" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acinetobacter baumannii m2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Tolia, N H]]		[[Category: Tolia, N H]]

OCA at 06:18, 24 July 2019

2019-07-24T06:18:30Z

←Older revision		Revision as of 06:18, 24 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6o38 ~~is ON HOLD until Paper Publication~~	+	==Structure of a chaperone-substrate complex==
-		+	<StructureSection load='6o38' size='340' side='right'caption='[[6o38]], [[Resolution\|resolution]] 2.59Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6o38]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O38 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o38 OCA], [http://pdbe.org/6o38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o38 RCSB], [http://www.ebi.ac.uk/pdbsum/6o38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o38 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Tolia, N H]]
		+	[[Category: Urusova, D V]]
		+	[[Category: Chaperone]]
		+	[[Category: Complex]]
		+	[[Category: Metalloprotease]]
		+	[[Category: Sugar binding protein]]
		+	[[Category: Type ii secretion]]

OCA at 04:33, 3 April 2019

2019-04-03T04:33:29Z

←Older revision		Revision as of 04:33, 3 April 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6o38 is ON HOLD	+	The entry 6o38 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6o38" [edit=sysop:move=sysop]

2019-03-06T05:43:43Z

Protected "6o38" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 6 March 2019

OCA: New page: '''Unreleased structure''' The entry 6o38 is ON HOLD Authors: Description: Category: Unreleased Structures

2019-03-06T05:43:41Z

New page: '''Unreleased structure''' The entry 6o38 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6o38 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]