6oy3 - Revision history

OCA at 09:25, 20 March 2024

2024-03-20T09:25:28Z

←Older revision		Revision as of 09:25, 20 March 2024
Line 3:		Line 3:
	<SX load='6oy3' size='340' side='right' viewer='molstar' caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6oy3' size='340' side='right' viewer='molstar' caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Fusarium_solani_subsp._pisi~~ Fusarium ~~solani subsp. pisi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_vanettenii_77-13-4 Fusarium vanettenii 77-13-4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=660122 Fusarium solani subsp. pisi])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [~~http~~://pdbe.org/6oy3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [https://pdbe.org/6oy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [https://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/C7Z7K1_FUSV7 C7Z7K1_FUSV7]
-	~~Both lipid and ion translocation by Ca(2+)-regulated TMEM16 transmembrane proteins utilizes a membrane-exposed hydrophilic groove~~. ~~Several conformations of the groove are observed in TMEM16 protein structures, but how these conformations form, and what functions they support, remains unknown~~. From analyses of atomistic molecular dynamics simulations of Ca(2+)-bound nhTMEM16 we find that the mechanism of a conformational transition of the groove from membrane-exposed to occluded from the membrane involves the repositioning of transmembrane helix 4 (TM4) following its disengagement from a TM3/~~TM4 interaction interface. Residue L302 is a key element in the hydrophobic TM3~~/TM4 interaction patch that braces the open-groove conformation, which should be changed by an L302A mutation. The structure of the L302A mutant determined by cryogenic electron microscopy (cryo-EM) reveals a partially closed groove that could translocate ions, but not lipids. This is corroborated with functional assays showing severely impaired lipid scrambling, but robust channel activity by L302A.	+

-	Dynamic modulation of the lipid translocation groove generates a conductive ion channel in Ca(2+)-bound nhTMEM16.,Khelashvili G, Falzone ME, Cheng X, Lee BC, Accardi A, Weinstein H Nat Commun. 2019 Oct 31;10(1):4972. doi: 10.1038/s41467-019-12865-4. PMID:31672969<ref>PMID:31672969</ref>	+	==See Also==
-		+	*[[Anoctamin 3D structures\|Anoctamin 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6oy3" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: Fusarium ~~solani subsp. pisi~~]]	+	[[Category: Fusarium vanettenii 77-13-4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Accardi, A]]	+	[[Category: Accardi A]]
-	[[Category: Falzone, M]]	+	[[Category: Falzone M]]
-	[[Category: Lee~~, B C]]~~	+	[[Category: Lee BC]]
-	~~[[Category: Anoactamin]]~~	+
-	~~[[Category: Lipid transport]]~~	+
-	~~[[Category: Scramblase]]~~	+
-	~~[[Category: Tmem16~~]]	+

OCA at 09:47, 18 November 2020

2020-11-18T09:47:35Z

←Older revision		Revision as of 09:47, 18 November 2020
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Both lipid and ion translocation by Ca(2+)-regulated TMEM16 transmembrane proteins utilizes a membrane-exposed hydrophilic groove. Several conformations of the groove are observed in TMEM16 protein structures, but how these conformations form, and what functions they support, remains unknown. From analyses of atomistic molecular dynamics simulations of Ca(2+)-bound nhTMEM16 we find that the mechanism of a conformational transition of the groove from membrane-exposed to occluded from the membrane involves the repositioning of transmembrane helix 4 (TM4) following its disengagement from a TM3/TM4 interaction interface. Residue L302 is a key element in the hydrophobic TM3/TM4 interaction patch that braces the open-groove conformation, which should be changed by an L302A mutation. The structure of the L302A mutant determined by cryogenic electron microscopy (cryo-EM) reveals a partially closed groove that could translocate ions, but not lipids. This is corroborated with functional assays showing severely impaired lipid scrambling, but robust channel activity by L302A.
		+
		+	Dynamic modulation of the lipid translocation groove generates a conductive ion channel in Ca(2+)-bound nhTMEM16.,Khelashvili G, Falzone ME, Cheng X, Lee BC, Accardi A, Weinstein H Nat Commun. 2019 Oct 31;10(1):4972. doi: 10.1038/s41467-019-12865-4. PMID:31672969<ref>PMID:31672969</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6oy3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 04:16, 11 April 2020

2020-04-11T04:16:26Z

←Older revision		Revision as of 04:16, 11 April 2020
Line 3:		Line 3:
	<SX load='6oy3' size='340' side='right' viewer='molstar' caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>		<SX load='6oy3' size='340' side='right' viewer='molstar' caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 23:19, 6 March 2020

2020-03-06T23:19:18Z

←Older revision		Revision as of 23:19, 6 March 2020
Line 1:		Line 1:

	==nhTMEM16 L302A +Ca2+ in nanodiscs==		==nhTMEM16 L302A +Ca2+ in nanodiscs==
-	<~~StructureSection~~ load='6oy3' size='340' side='right'caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>	+	<SX load='6oy3' size='340' side='right' viewer='molstar' caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>
Line 9:		Line 9:
	</table>		</table>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Fusarium solani subsp. pisi]]		[[Category: Fusarium solani subsp. pisi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 09:32, 8 January 2020

2020-01-08T09:32:56Z

←Older revision		Revision as of 09:32, 8 January 2020
Line 3:		Line 3:
	<StructureSection load='6oy3' size='340' side='right'caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>		<StructureSection load='6oy3' size='340' side='right'caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_solani_subsp._pisi Fusarium solani subsp. pisi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NECHADRAFT_66456 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=660122 Fusarium solani subsp. pisi])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium solani subsp. pisi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Accardi, A]]		[[Category: Accardi, A]]

OCA at 06:45, 6 November 2019

2019-11-06T06:45:20Z

←Older revision		Revision as of 06:45, 6 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6oy3 ~~is ON HOLD until Paper Publication~~	+	==nhTMEM16 L302A +Ca2+ in nanodiscs==
-		+	<StructureSection load='6oy3' size='340' side='right'caption='[[6oy3]], [[Resolution\|resolution]] 4.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6oy3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OY3 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy3 OCA], [http://pdbe.org/6oy3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy3 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy3 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Accardi, A]]
		+	[[Category: Falzone, M]]
		+	[[Category: Lee, B C]]
		+	[[Category: Anoactamin]]
		+	[[Category: Lipid transport]]
		+	[[Category: Scramblase]]
		+	[[Category: Tmem16]]

OCA at 07:09, 2 October 2019

2019-10-02T07:09:38Z

←Older revision		Revision as of 07:09, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6oy3 is ON HOLD	+	The entry 6oy3 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6oy3" [edit=sysop:move=sysop]

2019-05-22T04:05:38Z

Protected "6oy3" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6oy3 is ON HOLD Authors: Description: Category: Unreleased Structures

2019-05-22T04:05:36Z

New page: '''Unreleased structure''' The entry 6oy3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6oy3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]