6p49 - Revision history

OCA at 04:19, 11 April 2020

2020-04-11T04:19:22Z

←Older revision		Revision as of 04:19, 11 April 2020
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	<SX load='6p49' size='340' side='right' viewer='molstar' caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6p49' size='340' side='right' viewer='molstar' caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6P49 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P49 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p49 OCA], [http://pdbe.org/6p49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p49 RCSB], [http://www.ebi.ac.uk/pdbsum/6p49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p49 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p49 OCA], [http://pdbe.org/6p49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p49 RCSB], [http://www.ebi.ac.uk/pdbsum/6p49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p49 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:20, 6 March 2020

2020-03-06T23:20:25Z

←Older revision		Revision as of 23:20, 6 March 2020
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	==Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2==		==Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2==
-	<~~StructureSection~~ load='6p49' size='340' side='right'caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6p49' size='340' side='right' viewer='molstar' caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P49 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P49 FirstGlance]. <br>
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]

OCA at 12:20, 18 December 2019

2019-12-18T12:20:34Z

←Older revision		Revision as of 12:20, 18 December 2019
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	<StructureSection load='6p49' size='340' side='right'caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6p49' size='340' side='right'caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P49 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P49 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Ano6, Tmem16f ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p49 OCA], [http://pdbe.org/6p49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p49 RCSB], [http://www.ebi.ac.uk/pdbsum/6p49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p49 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p49 OCA], [http://pdbe.org/6p49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p49 RCSB], [http://www.ebi.ac.uk/pdbsum/6p49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p49 ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Cheng, T]]		[[Category: Cheng, T]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]

OCA at 06:25, 24 July 2019

2019-07-24T06:25:04Z

←Older revision		Revision as of 06:25, 24 July 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6p49 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2==
		+	<StructureSection load='6p49' size='340' side='right'caption='[[6p49]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6p49]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P49 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p49 OCA], [http://pdbe.org/6p49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p49 RCSB], [http://www.ebi.ac.uk/pdbsum/6p49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p49 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ANO6_MOUSE ANO6_MOUSE]] Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.<ref>PMID:21107324</ref> <ref>PMID:21908539</ref> <ref>PMID:22936354</ref> <ref>PMID:23021219</ref> <ref>PMID:23532839</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As a Ca(2+)-activated lipid scramblase and ion channel that mediates Ca(2+) influx, TMEM16F relies on both functions to facilitate extracellular vesicle generation, blood coagulation, and bone formation. How a bona fide ion channel scrambles lipids remains elusive. Our structural analyses revealed the coexistence of an intact channel pore and PIP2-dependent protein conformation changes leading to membrane distortion. Correlated to the extent of membrane distortion, many tightly bound lipids are slanted. Structure-based mutagenesis studies further reveal that neutralization of some lipid-binding residues or those near membrane distortion specifically alters the onset of lipid scrambling, but not Ca(2+) influx, thus identifying features outside of channel pore that are important for lipid scrambling. Together, our studies demonstrate that membrane distortion does not require open hydrophilic grooves facing the membrane interior and provide further evidence to suggest separate pathways for lipid scrambling and ion permeation.

-	~~Authors:~~ Feng, S., Dang, S., Han~~, T.W.~~, Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N.~~, Jan, L~~.Y.~~, Cheng, Y~~.	+	Cryo-EM Studies of TMEM16F Calcium-Activated Ion Channel Suggest Features Important for Lipid Scrambling.,Feng S, Dang S, Han TW, Ye W, Jin P, Cheng T, Li J, Jan YN, Jan LY, Cheng Y Cell Rep. 2019 Jul 9;28(2):567-579.e4. doi: 10.1016/j.celrep.2019.06.023. PMID:31291589<ref>PMID:31291589</ref>

-	~~Description: Cryo-EM structure~~ of ~~calcium-bound TMEM16F in nanodisc with supplement~~ of ~~PIP2 in Cl2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Dang~~, S]]	+	<div class="pdbe-citations 6p49" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cheng, T]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]
-	[[Category: ~~Han~~, ~~T.W~~]]	+	[[Category: Dang, S]]
	[[Category: Feng, S]]		[[Category: Feng, S]]
-	[[Category: ~~Jan~~, ~~Y.N~~]]	+	[[Category: Han, T W]]
-	[[Category: ~~Cheng~~, T]]	+	[[Category: Jan, L Y]]
-	[[Category: Li, J]]	+	[[Category: Jan, Y N]]
	[[Category: Jin, P]]		[[Category: Jin, P]]
-	[[Category: ~~Jan~~, ~~L.Y~~]]	+	[[Category: Li, J]]
	[[Category: Ye, W]]		[[Category: Ye, W]]
		+	[[Category: Membrane protein]]
		+	[[Category: Scramblase]]
		+	[[Category: Tmem16f]]

OCA at 08:54, 17 July 2019

2019-07-17T08:54:06Z

←Older revision		Revision as of 08:54, 17 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6p49 is ON HOLD	+	The entry 6p49 is ON HOLD until Paper Publication

	Authors: Feng, S., Dang, S., Han, T.W., Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N., Jan, L.Y., Cheng, Y.		Authors: Feng, S., Dang, S., Han, T.W., Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N., Jan, L.Y., Cheng, Y.
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	Description: Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2		Description: Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Dang, S]]
	[[Category: Cheng, Y]]		[[Category: Cheng, Y]]
		+	[[Category: Han, T.W]]
	[[Category: Feng, S]]		[[Category: Feng, S]]
	[[Category: Jan, Y.N]]		[[Category: Jan, Y.N]]
		+	[[Category: Cheng, T]]
	[[Category: Li, J]]		[[Category: Li, J]]
	[[Category: Jin, P]]		[[Category: Jin, P]]
-	[[Category: Ye, W]]
-	[[Category: Dang, S]]
-	[[Category: Han, T.W]]
-	[[Category: Cheng, T]]
	[[Category: Jan, L.Y]]		[[Category: Jan, L.Y]]
		+	[[Category: Ye, W]]

OCA: Protected "6p49" [edit=sysop:move=sysop]

2019-06-05T21:12:59Z

Protected "6p49" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:12, 5 June 2019

OCA: New page: '''Unreleased structure''' The entry 6p49 is ON HOLD Authors: Feng, S., Dang, S., Han, T.W., Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N., Jan, L.Y., Cheng, Y. Description: Cryo-EM st...

2019-06-05T21:12:58Z

New page: '''Unreleased structure''' The entry 6p49 is ON HOLD Authors: Feng, S., Dang, S., Han, T.W., Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N., Jan, L.Y., Cheng, Y. Description: Cryo-EM st...

New page

'''Unreleased structure'''

The entry 6p49 is ON HOLD

Authors: Feng, S., Dang, S., Han, T.W., Ye, W., Jin, P., Cheng, T., Li, J., Jan, Y.N., Jan, L.Y., Cheng, Y.

Description: Cryo-EM structure of calcium-bound TMEM16F in nanodisc with supplement of PIP2 in Cl2
[[Category: Unreleased Structures]]
[[Category: Cheng, Y]]
[[Category: Feng, S]]
[[Category: Jan, Y.N]]
[[Category: Li, J]]
[[Category: Jin, P]]
[[Category: Ye, W]]
[[Category: Dang, S]]
[[Category: Han, T.W]]
[[Category: Cheng, T]]
[[Category: Jan, L.Y]]