6p65 - Revision history

OCA at 04:21, 11 April 2020

2020-04-11T04:21:37Z

←Older revision		Revision as of 04:21, 11 April 2020
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	<SX load='6p65' size='340' side='right' viewer='molstar' caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>		<SX load='6p65' size='340' side='right' viewer='molstar' caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6P65 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6P65 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:38, 1 April 2020

2020-04-01T09:38:10Z

←Older revision		Revision as of 09:38, 1 April 2020
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The elicitation of broadly neutralizing antibodies (bNAbs) against the HIV-1 envelope glycoprotein (Env) trimer remains a major vaccine challenge. Most cross-conserved protein determinants are occluded by self-N-glycan shielding, limiting B cell recognition of the underlying polypeptide surface. The exceptions to the contiguous glycan shield include the conserved receptor CD4 binding site (CD4bs) and glycoprotein (gp)41 elements proximal to the furin cleavage site. Accordingly, we performed heterologous trimer-liposome prime:boosting in rabbits to drive B cells specific for cross-conserved sites. To preferentially expose the CD4bs to B cells, we eliminated proximal N-glycans while maintaining the native-like state of the cleavage-independent NFL trimers, followed by gradual N-glycan restoration coupled with heterologous boosting. This approach successfully elicited CD4bs-directed, cross-neutralizing Abs, including one targeting a unique glycan-protein epitope and a bNAb (87% breadth) directed to the gp120:gp41 interface, both resolved by high-resolution cryoelectron microscopy. This study provides proof-of-principle immunogenicity toward eliciting bNAbs by vaccination.
		+
		+	Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability.,Dubrovskaya V, Tran K, Ozorowski G, Guenaga J, Wilson R, Bale S, Cottrell CA, Turner HL, Seabright G, O'Dell S, Torres JL, Yang L, Feng Y, Leaman DP, Vazquez Bernat N, Liban T, Louder M, McKee K, Bailer RT, Movsesyan A, Doria-Rose NA, Pancera M, Karlsson Hedestam GB, Zwick MB, Crispin M, Mascola JR, Ward AB, Wyatt RT Immunity. 2019 Nov 19;51(5):915-929.e7. doi: 10.1016/j.immuni.2019.10.008. Epub, 2019 Nov 12. PMID:31732167<ref>PMID:31732167</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6p65" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 23:21, 6 March 2020

2020-03-06T23:21:11Z

←Older revision		Revision as of 23:21, 6 March 2020
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	==HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding==		==HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding==
-	<~~StructureSection~~ load='6p65' size='340' side='right'caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>	+	<SX load='6p65' size='340' side='right' viewer='molstar' caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P65 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P65 FirstGlance]. <br>
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	</table>		</table>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: European rabbit]]		[[Category: European rabbit]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 12:21, 18 December 2019

2019-12-18T12:21:35Z

←Older revision		Revision as of 12:21, 18 December 2019
Line 3:		Line 3:
	<StructureSection load='6p65' size='340' side='right'caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>		<StructureSection load='6p65' size='340' side='right'caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P65 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P65 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: European rabbit]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ozorowski, G]]		[[Category: Ozorowski, G]]

OCA at 15:29, 20 November 2019

2019-11-20T15:29:43Z

←Older revision		Revision as of 15:29, 20 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6p65 is ON HOLD~~	+	==HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding==
-		+	<StructureSection load='6p65' size='340' side='right'caption='[[6p65]], [[Resolution\|resolution]] 3.94Å' scene=''>
-	~~Authors: Ozorowski, G., Torres, J.L., Ward, A.B.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6p65]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6P65 FirstGlance]. <br>
-	~~Description:~~ HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6p65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p65 OCA], [http://pdbe.org/6p65 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6p65 RCSB], [http://www.ebi.ac.uk/pdbsum/6p65 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6p65 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Ward~~, A.B]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ozorowski, G]]		[[Category: Ozorowski, G]]
-	[[Category: Torres, J.L]]	+	[[Category: Torres, J L]]
		+	[[Category: Ward, A B]]
		+	[[Category: Hiv-1]]
		+	[[Category: Neutralizing antibody]]
		+	[[Category: Rabbit antibody]]
		+	[[Category: Viral protein]]
		+	[[Category: Viral protein-immune system complex]]

OCA: Protected "6p65" [edit=sysop:move=sysop]

2019-06-12T04:03:31Z

Protected "6p65" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6p65 is ON HOLD Authors: Ozorowski, G., Torres, J.L., Ward, A.B. Description: HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antig...

2019-06-12T04:03:30Z

New page: '''Unreleased structure''' The entry 6p65 is ON HOLD Authors: Ozorowski, G., Torres, J.L., Ward, A.B. Description: HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antig...

New page

'''Unreleased structure'''

The entry 6p65 is ON HOLD

Authors: Ozorowski, G., Torres, J.L., Ward, A.B.

Description: HIV Env 16055 NFL TD 2CC+ in complex with antibody 1C2 fragment antigen binding
[[Category: Unreleased Structures]]
[[Category: Ward, A.B]]
[[Category: Ozorowski, G]]
[[Category: Torres, J.L]]