OCA at 12:28, 18 December 2019

2019-12-18T12:28:05Z

←Older revision		Revision as of 12:28, 18 December 2019
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	</div>		</div>
	<div class="pdbe-citations 6pkl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6pkl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Proteinase\|Proteinase]]
		+	*[[Proteinase 3D structures\|Proteinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:31, 16 October 2019

2019-10-16T07:31:38Z

←Older revision		Revision as of 07:31, 16 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6pkl is ~~ON HOLD until Paper~~ Publication	+	==MicroED structure of proteinase K from an uncoated, single lamella at 2.59A resolution (#7)==
		+	<StructureSection load='6pkl' size='340' side='right'caption='[[6pkl]], [[Resolution\|resolution]] 2.59Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pkl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Parengyodontium_album Parengyodontium album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PKL FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Peptidase_K Peptidase K], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.64 3.4.21.64] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pkl OCA], [http://pdbe.org/6pkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pkl RCSB], [http://www.ebi.ac.uk/pdbsum/6pkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pkl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRTK_PARAQ PRTK_PARAQ]] Hydrolyzes keratin at aromatic and hydrophobic residues.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED.

-	~~Authors~~:	+	Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.,Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T Structure. 2019 Oct 1;27(10):1594-1600.e2. doi: 10.1016/j.str.2019.07.004. Epub, 2019 Aug 15. PMID:31422911<ref>PMID:31422911</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6pkl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Parengyodontium album]]
		+	[[Category: Peptidase K]]
		+	[[Category: Gonen, T]]
		+	[[Category: Hattne, J]]
		+	[[Category: Jensen, G J]]
		+	[[Category: Martynowycz, M W]]
		+	[[Category: Zhao, W]]
		+	[[Category: Hydrolase]]

OCA at 04:26, 24 July 2019

2019-07-24T04:26:14Z

←Older revision		Revision as of 04:26, 24 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pkl is ON HOLD	+	The entry 6pkl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6pkl" [edit=sysop:move=sysop]

2019-07-10T04:11:34Z

Protected "6pkl" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 10 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pkl is ON HOLD Authors: Description: Category: Unreleased Structures

2019-07-10T04:11:32Z

New page: '''Unreleased structure''' The entry 6pkl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6pkl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6pkl - Revision history

OCA at 12:28, 18 December 2019

OCA at 07:31, 16 October 2019

OCA at 04:26, 24 July 2019

OCA: Protected "6pkl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6pkl is ON HOLD Authors: Description: Category: Unreleased Structures