6pua - Revision history

OCA at 07:36, 11 October 2023

2023-10-11T07:36:08Z

←Older revision		Revision as of 07:36, 11 October 2023
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	<StructureSection load='6pua' size='340' side='right'caption='[[6pua]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6pua' size='340' side='right'caption='[[6pua]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pua]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Vibch Vibch~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PUA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pua]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PUA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eev\|3eev]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_A0300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pua OCA], [https://pdbe.org/6pua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pua RCSB], [https://www.ebi.ac.uk/pdbsum/6pua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pua ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pua OCA], [~~http~~://pdbe.org/6pua PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pua RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pua PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pua ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9KMN1_VIBCH Q9KMN1_VIBCH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6pua" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6pua" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chloramphenicol acetyltransferase 3D structures\|Chloramphenicol acetyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibch]]~~	+	[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Joachimiak A]]
-	[[Category: Joachimiak, A]]	+	[[Category: Kim Y]]
-	[[Category: Kim, Y]]	+	[[Category: Maltseva N]]
-	[[Category: Maltseva, N]]	+	[[Category: Stam J]]
-	[[Category: Stam, J]]	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Hexapeptide repeat]]~~	+
-	~~[[Category: Left-handed beta helix]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 18:44, 11 December 2019

2019-12-11T18:44:58Z

←Older revision		Revision as of 18:44, 11 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6pua is ~~ON HOLD~~	+	==The 2.0 A Crystal Structure of the Type B Chloramphenicol Acetyltransferase from Vibrio cholerae==
		+	<StructureSection load='6pua' size='340' side='right'caption='[[6pua]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pua]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PUA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3eev\|3eev]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VC_A0300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pua OCA], [http://pdbe.org/6pua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pua RCSB], [http://www.ebi.ac.uk/pdbsum/6pua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pua ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chloramphenicol acetyltransferases (CATs) were among the first antibiotic resistance enzymes identified and have long been studied as model enzymes for examining plasmid-mediated antibiotic resistance. These enzymes acetylate the antibiotic chloramphenicol, which renders it incapable of inhibiting bacterial protein synthesis. CATs can be classified into different types: Type A CATs are known to be important for antibiotic resistance to chloramphenicol and fusidic acid. Type B CATs are often called xenobiotic acetyltransferases and adopt a similar structural fold to streptogramin acetyltransferases, which are known to be critical for streptogramin antibiotic resistance. Type C CATs have recently been identified and can also acetylate chloramphenicol, but their roles in antibiotic resistance are largely unknown. Here, we structurally and kinetically characterized three Vibrio CAT proteins from a nonpathogenic species (Aliivibrio fisheri) and two important human pathogens (Vibrio cholerae and Vibrio vulnificus). We found all three proteins, including one in a superintegron (V. cholerae), acetylated chloramphenicol, but did not acetylate aminoglycosides or dalfopristin. We also determined the 3D crystal structures of these CATs alone and in complex with crystal violet and taurocholate. These compounds are known inhibitors of Type A CATs, but have not been explored in Type B and Type C CATs. Based on sequence, structure, and kinetic analysis, we concluded that the V. cholerae and V. vulnificus CATs belong to the Type B class and the A. fisheri CAT belongs to the Type C class. Ultimately, our results provide a framework for studying the evolution of antibiotic resistance gene acquisition and chloramphenicol acetylation in Vibrio and other species.

-	~~Authors: Kim~~, Y., ~~Maltseva~~, N., ~~Stam~~, J., ~~Joachimiak~~, A., ~~Center for Structural Genomics of Infectious Diseases (CSGID)~~	+	Structural and functional characterization of three Type B and C chloramphenicol acetyltransferases from Vibrio species.,Alcala A, Ramirez G, Solis A, Kim Y, Tan K, Luna O, Nguyen K, Vazquez D, Ward M, Zhou M, Mulligan R, Maltseva N, Kuhn ML Protein Sci. 2019 Nov 24. doi: 10.1002/pro.3793. PMID:31762145<ref>PMID:31762145</ref>

-	~~Description: The 2.0 A Crystal Structure~~ of the ~~Type B Chloramphenicol Acetyltransferase from Vibrio cholerae~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Maltseva, N~~]]	+	<div class="pdbe-citations 6pua" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibch]]
		+	[[Category: Structural genomic]]
	[[Category: Joachimiak, A]]		[[Category: Joachimiak, A]]
-	[[Category: Stam, J]]
-	[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
	[[Category: Kim, Y]]		[[Category: Kim, Y]]
		+	[[Category: Maltseva, N]]
		+	[[Category: Stam, J]]
		+	[[Category: Csgid]]
		+	[[Category: Hexapeptide repeat]]
		+	[[Category: Left-handed beta helix]]
		+	[[Category: Transferase]]

OCA: Protected "6pua" [edit=sysop:move=sysop]

2019-07-24T04:35:58Z

Protected "6pua" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 24 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pua is ON HOLD Authors: Kim, Y., Maltseva, N., Stam, J., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID) Description...

2019-07-24T04:35:56Z

New page: '''Unreleased structure''' The entry 6pua is ON HOLD Authors: Kim, Y., Maltseva, N., Stam, J., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID) Description...

New page

'''Unreleased structure'''

The entry 6pua is ON HOLD

Authors: Kim, Y., Maltseva, N., Stam, J., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: The 2.0 A Crystal Structure of the Type B Chloramphenicol Acetyltransferase from Vibrio cholerae
[[Category: Unreleased Structures]]
[[Category: Maltseva, N]]
[[Category: Joachimiak, A]]
[[Category: Stam, J]]
[[Category: Center For Structural Genomics Of Infectious Diseases (Csgid)]]
[[Category: Kim, Y]]