6pwb - Revision history

OCA at 09:27, 20 March 2024

2024-03-20T09:27:42Z

←Older revision		Revision as of 09:27, 20 March 2024
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	<StructureSection load='6pwb' size='340' side='right'caption='[[6pwb]], [[Resolution\|resolution]] 9.83Å' scene=''>		<StructureSection load='6pwb' size='340' side='right'caption='[[6pwb]], [[Resolution\|resolution]] 9.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6pwb]] is a 163 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Leptospira_biflexa_serovar_patoc_~~(~~strain_patoc_1_/_atcc_23582_/_paris~~) Leptospira biflexa serovar ~~patoc (~~strain ~~patoc~~ 1 ~~/ atcc 23582 / paris~~)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6PWB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6pwb]] is a 163 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_biflexa_serovar_Patoc_strain_'Patoc_1_(Paris)' Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PWB FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5wjt\|5wjt]]~~, [[~~6nqz~~\|~~6nqz]], [[6nqw\|6nqw]], [[6nqx\|6nqx]], [[6nqy\|6nqy~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 9.83Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6pwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwb OCA], [~~http~~://pdbe.org/6pwb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6pwb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6pwb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwb OCA], [https://pdbe.org/6pwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pwb RCSB], [https://www.ebi.ac.uk/pdbsum/6pwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/B0SSZ5_LEPBP B0SSZ5_LEPBP]] Component of the core of the flagella.[RuleBase:RU362073]	+	[https://www.uniprot.org/uniprot/B0SSZ5_LEPBP B0SSZ5_LEPBP] Component of the core of the flagella.[RuleBase:RU362073]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled 'endoflagella' that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirochete Leptospira by integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of this spirochete flagellum.	+

-	An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete.,Gibson KH, Trajtenberg F, Wunder EA, Brady MR, San Martin F, Mechaly A, Shang Z, Liu J, Picardeau M, Ko A, Buschiazzo A, Sindelar CV Elife. 2020 Mar 11;9. pii: 53672. doi: 10.7554/eLife.53672. PMID:32157997<ref>PMID:32157997</ref>	+	==See Also==
-		+	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+	*[[Flagellin 3D structures\|Flagellin 3D structures]]
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6pwb" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Buschiazzo, A]]	+	[[Category: Buschiazzo A]]
-	[[Category: Gibson~~, K H~~]]	+	[[Category: Gibson KH]]
-	[[Category: ~~Martin, F San~~]]	+	[[Category: Mechaly A]]
-	[[Category: ~~Mechaly, A~~]]	+	[[Category: San Martin F]]
-	[[Category: Sindelar~~, C V~~]]	+	[[Category: Sindelar CV]]
-	[[Category: Trajtenberg, F]]	+	[[Category: Trajtenberg F]]
-	~~[[Category: Bacterial flagella]]~~	+
-	~~[[Category: Fcpa]]~~	+
-	~~[[Category: Fcpb]]~~	+
-	~~[[Category: Flaa]]~~	+
-	~~[[Category: Flab]]~~	+
-	~~[[Category: Leptospira]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 10:14, 27 March 2020

2020-03-27T10:14:23Z

←Older revision		Revision as of 10:14, 27 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6pwb is ~~ON HOLD~~	+	==Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging==
		+	<StructureSection load='6pwb' size='340' side='right'caption='[[6pwb]], [[Resolution\|resolution]] 9.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6pwb]] is a 163 chain structure with sequence from [http://en.wikipedia.org/wiki/Leptospira_biflexa_serovar_patoc_(strain_patoc_1_/_atcc_23582_/_paris) Leptospira biflexa serovar patoc (strain patoc 1 / atcc 23582 / paris)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PWB FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5wjt\|5wjt]], [[6nqz\|6nqz]], [[6nqw\|6nqw]], [[6nqx\|6nqx]], [[6nqy\|6nqy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6pwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pwb OCA], [http://pdbe.org/6pwb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6pwb RCSB], [http://www.ebi.ac.uk/pdbsum/6pwb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6pwb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/B0SSZ5_LEPBP B0SSZ5_LEPBP]] Component of the core of the flagella.[RuleBase:RU362073]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations of the entire cell. Motility is powered by the rotation of supercoiled 'endoflagella' that wrap around the cell body, confined within the periplasmic space. To investigate the structural basis of flagellar supercoiling, which is critical for motility, we determined the structure of native flagellar filaments from the spirochete Leptospira by integrating high-resolution cryo-electron tomography and X-ray crystallography. We show that these filaments are coated by a highly asymmetric, multi-component sheath layer, contrasting with flagellin-only homopolymers previously observed in exoflagellated bacteria. Distinct sheath proteins localize to the filament inner and outer curvatures to define the supercoiling geometry, explaining a key functional attribute of this spirochete flagellum.

-	~~Authors: Gibson, K.H~~., ~~Sindelar, C.V.~~, Trajtenberg, F., ~~Buschiazzo~~, A., San Martin, F., Mechaly, A.	+	An asymmetric sheath controls flagellar supercoiling and motility in the leptospira spirochete.,Gibson KH, Trajtenberg F, Wunder EA, Brady MR, San Martin F, Mechaly A, Shang Z, Liu J, Picardeau M, Ko A, Buschiazzo A, Sindelar CV Elife. 2020 Mar 11;9. pii: 53672. doi: 10.7554/eLife.53672. PMID:32157997<ref>PMID:32157997</ref>

-	~~Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB~~, ~~into~~ a ~~10 Angstrom structure~~ of the ~~asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Sindelar, C.V]]~~	+	<div class="pdbe-citations 6pwb" style="background-color:#fffaf0;"></div>
-	~~[[Category: San Martin, F]]~~	+	== References ==
-	~~[[Category: Gibson, K.H]]~~	+	<references/>
-	[[Category: ~~Mechaly, A~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Buschiazzo, A]]		[[Category: Buschiazzo, A]]
		+	[[Category: Gibson, K H]]
		+	[[Category: Martin, F San]]
		+	[[Category: Mechaly, A]]
		+	[[Category: Sindelar, C V]]
	[[Category: Trajtenberg, F]]		[[Category: Trajtenberg, F]]
		+	[[Category: Bacterial flagella]]
		+	[[Category: Fcpa]]
		+	[[Category: Fcpb]]
		+	[[Category: Flaa]]
		+	[[Category: Flab]]
		+	[[Category: Leptospira]]
		+	[[Category: Structural protein]]

OCA at 08:23, 18 March 2020

2020-03-18T08:23:27Z

←Older revision		Revision as of 08:23, 18 March 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pwb is ON HOLD ~~until Paper Publication~~	+	The entry 6pwb is ON HOLD

	Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.		Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.
Line 7:		Line 7:
	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging		Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Buschiazzo, A]]
	[[Category: Sindelar, C.V]]		[[Category: Sindelar, C.V]]
	[[Category: San Martin, F]]		[[Category: San Martin, F]]
	[[Category: Gibson, K.H]]		[[Category: Gibson, K.H]]
	[[Category: Mechaly, A]]		[[Category: Mechaly, A]]
		+	[[Category: Buschiazzo, A]]
	[[Category: Trajtenberg, F]]		[[Category: Trajtenberg, F]]

OCA at 05:33, 4 March 2020

2020-03-04T05:33:00Z

←Older revision		Revision as of 05:33, 4 March 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pwb is ON HOLD	+	The entry 6pwb is ON HOLD until Paper Publication

	Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.		Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.
Line 7:		Line 7:
	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging		Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Buschiazzo, A]]
	[[Category: Sindelar, C.V]]		[[Category: Sindelar, C.V]]
	[[Category: San Martin, F]]		[[Category: San Martin, F]]
	[[Category: Gibson, K.H]]		[[Category: Gibson, K.H]]
	[[Category: Mechaly, A]]		[[Category: Mechaly, A]]
-	[[Category: Buschiazzo, A]]
	[[Category: Trajtenberg, F]]		[[Category: Trajtenberg, F]]

OCA at 02:37, 13 February 2020

2020-02-13T02:37:11Z

←Older revision		Revision as of 02:37, 13 February 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pwb is ON HOLD ~~until Paper Publication~~	+	The entry 6pwb is ON HOLD

	Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.		Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.
Line 7:		Line 7:
	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging		Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Buschiazzo, A]]
	[[Category: Sindelar, C.V]]		[[Category: Sindelar, C.V]]
	[[Category: San Martin, F]]		[[Category: San Martin, F]]
	[[Category: Gibson, K.H]]		[[Category: Gibson, K.H]]
	[[Category: Mechaly, A]]		[[Category: Mechaly, A]]
		+	[[Category: Buschiazzo, A]]
	[[Category: Trajtenberg, F]]		[[Category: Trajtenberg, F]]

OCA at 07:14, 25 December 2019

2019-12-25T07:14:02Z

←Older revision		Revision as of 07:14, 25 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6pwb is ON HOLD	+	The entry 6pwb is ON HOLD until Paper Publication

	Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.		Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.
Line 7:		Line 7:
	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging		Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Buschiazzo, A]]
	[[Category: Sindelar, C.V]]		[[Category: Sindelar, C.V]]
	[[Category: San Martin, F]]		[[Category: San Martin, F]]
	[[Category: Gibson, K.H]]		[[Category: Gibson, K.H]]
	[[Category: Mechaly, A]]		[[Category: Mechaly, A]]
-	[[Category: Buschiazzo, A]]
	[[Category: Trajtenberg, F]]		[[Category: Trajtenberg, F]]

OCA at 13:46, 28 August 2019

2019-08-28T13:46:35Z

←Older revision		Revision as of 13:46, 28 August 2019
Line 5:		Line 5:
	Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.		Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.

-	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging.	+	Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Sindelar, C.V]]		[[Category: Sindelar, C.V]]

OCA: Protected "6pwb" [edit=sysop:move=sysop]

2019-07-31T04:48:33Z

Protected "6pwb" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6pwb is ON HOLD Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A. Description: Rigid body fitting...

2019-07-31T04:48:32Z

New page: '''Unreleased structure''' The entry 6pwb is ON HOLD Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A. Description: Rigid body fitting...

New page

'''Unreleased structure'''

The entry 6pwb is ON HOLD

Authors: Gibson, K.H., Sindelar, C.V., Trajtenberg, F., Buschiazzo, A., San Martin, F., Mechaly, A.

Description: Rigid body fitting of flagellin FlaB, and flagellar coiling proteins, FcpA and FcpB, into a 10 Angstrom structure of the asymmetric flagellar filament purified from Leptospira biflexa Patoc WT cells resolved via subtomogram averaging.
[[Category: Unreleased Structures]]
[[Category: Sindelar, C.V]]
[[Category: San Martin, F]]
[[Category: Gibson, K.H]]
[[Category: Mechaly, A]]
[[Category: Buschiazzo, A]]
[[Category: Trajtenberg, F]]