6q5m - Revision history

OCA at 06:57, 23 May 2019

2019-05-23T06:57:41Z

←Older revision		Revision as of 06:57, 23 May 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6q5m is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24Dab==
		+	<StructureSection load='6q5m' size='340' side='right'caption='[[6q5m]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6q5m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Q5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Q5M FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=DAB:2,4-DIAMINOBUTYRIC+ACID'>DAB</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6q5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6q5m OCA], [http://pdbe.org/6q5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6q5m RCSB], [http://www.ebi.ac.uk/pdbsum/6q5m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6q5m ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The association of amphipathic alpha helices in water leads to alpha-helical-bundle protein structures. However, the driving force for this-the hydrophobic effect-is not specific and does not define the number or the orientation of helices in the associated state. Rather, this is achieved through deeper sequence-to-structure relationships, which are increas-ingly being discerned. For example, for one structurally extreme but nevertheless ubiquitous class of bundle-the alpha-helical coiled coils-relationships have been established that discriminate between all-parallel dimers, trimers and tetramers. Association states above this are known, as are antiparallel and mixed arrangements of the helices. However, these alternative states are less-well understood. Here, we describe a synthetic-peptide system that switches be-tween parallel hexamers and various up-down-up-down tetramers in response to single-amino-acid changes and solution conditions. The main accessible states of each peptide variant are characterized fully in solution and, in most cases, to high resolution with X-ray crystal structures. Analysis and inspection of these structures helps rationalize the different states formed. This navigation of the structural landscape of alpha-helical coiled coils above the dimers and tri-mers that dominate in nature has allowed us to design rationally a well-defined and hyperstable antiparallel coiled-coil tetramer (apCC-Tet). This robust de novo protein provides another scaffold for further structural and functional designs in protein engineering and synthetic biology.

-	~~Authors:~~ Wood~~, C.W.~~, Beesley, ~~J.L.~~, ~~Rhys, G.G.~~, Brady, ~~R.L.~~, Woolfson~~, D~~.N.	+	Navigating the structural landscape of de novo alpha-helical bundles.,Rhys GG, Wood CW, Beesley JL, Zaccai NR, Burton A, Brady RL, Thomson AR, Woolfson DN J Am Chem Soc. 2019 May 8. doi: 10.1021/jacs.8b13354. PMID:31066556<ref>PMID:31066556</ref>

-	~~Description: Crystal structure of~~ a CC-~~Hex mutant that forms an antiparallel four~~-~~helix coiled coil CC-Hex*-L24Dab~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Rhys, G.G]]	+	<div class="pdbe-citations 6q5m" style="background-color:#fffaf0;"></div>
-	[[Category: Wood, C.W]]	+	== References ==
-	[[Category: ~~Brady~~, ~~R.L~~]]	+	<references/>
-	[[Category: ~~Woolfson, D.N~~]]	+	__TOC__
-	[[Category: ~~Beesley, J.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Beesley, J L]]
		+	[[Category: Brady, R L]]
		+	[[Category: Rhys, G G]]
		+	[[Category: Wood, C W]]
		+	[[Category: Woolfson, D N]]
		+	[[Category: Antiparallel]]
		+	[[Category: Cc-hex]]
		+	[[Category: Coiled coil]]
		+	[[Category: De novo protein]]
		+	[[Category: Synthetic]]
		+	[[Category: Tetramer]]

OCA at 05:08, 27 March 2019

2019-03-27T05:08:53Z

←Older revision		Revision as of 05:08, 27 March 2019
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	Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N.		Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N.

-	Description: Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-L24Dab	+	Description: Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex*-L24Dab
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Rhys, G.G]]
		+	[[Category: Wood, C.W]]
	[[Category: Brady, R.L]]		[[Category: Brady, R.L]]
	[[Category: Woolfson, D.N]]		[[Category: Woolfson, D.N]]
	[[Category: Beesley, J.L]]		[[Category: Beesley, J.L]]
-	[[Category: Rhys, G.G]]
-	[[Category: Wood, C.W]]

OCA at 05:00, 2 January 2019

2019-01-02T05:00:34Z

←Older revision		Revision as of 05:00, 2 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6q5m is ON HOLD	+	The entry 6q5m is ON HOLD until Paper Publication

	Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N.		Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N.
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	Description: Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-L24Dab		Description: Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-L24Dab
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Rhys, G.G]]
-	[[Category: Wood, C.W]]
	[[Category: Brady, R.L]]		[[Category: Brady, R.L]]
	[[Category: Woolfson, D.N]]		[[Category: Woolfson, D.N]]
	[[Category: Beesley, J.L]]		[[Category: Beesley, J.L]]
		+	[[Category: Rhys, G.G]]
		+	[[Category: Wood, C.W]]

OCA: Protected "6q5m" [edit=sysop:move=sysop]

2018-12-19T06:44:08Z

Protected "6q5m" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 19 December 2018

OCA: New page: '''Unreleased structure''' The entry 6q5m is ON HOLD Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N. Description: Crystal structure of a CC-Hex mutant that ...

2018-12-19T06:44:07Z

New page: '''Unreleased structure''' The entry 6q5m is ON HOLD Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N. Description: Crystal structure of a CC-Hex mutant that ...

New page

'''Unreleased structure'''

The entry 6q5m is ON HOLD

Authors: Wood, C.W., Beesley, J.L., Rhys, G.G., Brady, R.L., Woolfson, D.N.

Description: Crystal structure of a CC-Hex mutant that forms an antiparallel four-helix coiled coil CC-Hex-L24Dab
[[Category: Unreleased Structures]]
[[Category: Rhys, G.G]]
[[Category: Wood, C.W]]
[[Category: Brady, R.L]]
[[Category: Woolfson, D.N]]
[[Category: Beesley, J.L]]