OCA at 17:10, 26 April 2023

2023-04-26T17:10:31Z

←Older revision		Revision as of 17:10, 26 April 2023
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	==Structural investigation of the TasA anchoring protein TapA from Bacillus subtilis==		==Structural investigation of the TasA anchoring protein TapA from Bacillus subtilis==
-	<StructureSection load='6qay' size='340' side='right'caption='[[6qay~~]], [[NMR_Ensembles_of_Models \| 15 NMR models~~]]' scene=''>	+	<StructureSection load='6qay' size='340' side='right'caption='[[6qay]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qay]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QAY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qay]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QAY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qay OCA], [~~http~~://pdbe.org/6qay PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qay RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qay PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qay ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qay OCA], [https://pdbe.org/6qay PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qay RCSB], [https://www.ebi.ac.uk/pdbsum/6qay PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qay ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TAPA_BACSU TAPA_BACSU]] Required for biofilm formation (PubMed:16430695, PubMed:16430696, PubMed:21477127, PubMed:24488317). Required for the proper anchoring and polymerization of TasA amyloid fibers at the cell surface (PubMed:16430696, PubMed:21477127, PubMed:24488317). Is also a minor component of TasA fibers (PubMed:21477127).<ref>PMID:16430695</ref> <ref>PMID:16430696</ref> <ref>PMID:21477127</ref> <ref>PMID:24488317</ref>	+	[https://www.uniprot.org/uniprot/TAPA_BACSU TAPA_BACSU] Required for biofilm formation (PubMed:16430695, PubMed:16430696, PubMed:21477127, PubMed:24488317). Required for the proper anchoring and polymerization of TasA amyloid fibers at the cell surface (PubMed:16430696, PubMed:21477127, PubMed:24488317). Is also a minor component of TasA fibers (PubMed:21477127).<ref>PMID:16430695</ref> <ref>PMID:16430696</ref> <ref>PMID:21477127</ref> <ref>PMID:24488317</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Studying mechanisms of bacterial biofilm generation is of vital importance to understanding bacterial cell-cell communication, multicellular cohabitation principles, and the higher resilience of microorganisms in a biofilm against antibiotics. Biofilms of the nonpathogenic, gram-positive soil bacterium Bacillus subtilis serve as a model system with biotechnological potential toward plant protection. Its major extracellular matrix protein components are TasA and TapA. The nature of TasA filaments has been of debate, and several forms, amyloidic and non-Thioflavin T-stainable have been observed. Here, we present the three-dimensional structure of TapA and uncover the mechanism of TapA-supported growth of nonamyloidic TasA filaments. By analytical ultracentrifugation and NMR, we demonstrate TapA-dependent acceleration of filament formation from solutions of folded TasA. Solid-state NMR revealed intercalation of the N-terminal TasA peptide segment into subsequent protomers to form a filament composed of beta-sandwich subunits. The secondary structure around the intercalated N-terminal strand beta0 is conserved between filamentous TasA and the Fim and Pap proteins, which form bacterial type I pili, demonstrating such construction principles in a gram-positive organism. Analogous to the chaperones of the chaperone-usher pathway, the role of TapA is in donating its N terminus to serve for TasA folding into an Ig domain-similar filament structure by donor-strand complementation. According to NMR and since the V-set Ig fold of TapA is already complete, its participation within a filament beyond initiation is unlikely. Intriguingly, the most conserved residues in TasA-like proteins (camelysines) of Bacillaceae are located within the protomer interface.
		+
		+	TapA acts as specific chaperone in TasA filament formation by strand complementation.,Roske Y, Lindemann F, Diehl A, Cremer N, Higman VA, Schlegel B, Leidert M, Driller K, Turgay K, Schmieder P, Heinemann U, Oschkinat H Proc Natl Acad Sci U S A. 2023 Apr 25;120(17):e2217070120. doi: , 10.1073/pnas.2217070120. Epub 2023 Apr 17. PMID:37068239<ref>PMID:37068239</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qay" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Diehl, A]]	+	[[Category: Diehl A]]
-	[[Category: Higman~~, V A~~]]	+	[[Category: Higman VA]]
-	[[Category: Oschkinat, H]]	+	[[Category: Oschkinat H]]
-	[[Category: Schmieder, P]]	+	[[Category: Schmieder P]]
-	~~[[Category: Anchoring]]~~	+
-	~~[[Category: Biofilm]]~~	+
-	~~[[Category: Protein fibril]]~~	+
-	~~[[Category: Tapa]]~~	+
-	~~[[Category: Tasa~~]]	+

OCA at 15:34, 29 January 2020

2020-01-29T15:34:00Z

←Older revision		Revision as of 15:34, 29 January 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6qay ~~is ON HOLD~~	+	==Structural investigation of the TasA anchoring protein TapA from Bacillus subtilis==
-		+	<StructureSection load='6qay' size='340' side='right'caption='[[6qay]], [[NMR_Ensembles_of_Models \| 15 NMR models]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qay]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QAY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qay OCA], [http://pdbe.org/6qay PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qay RCSB], [http://www.ebi.ac.uk/pdbsum/6qay PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qay ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TAPA_BACSU TAPA_BACSU]] Required for biofilm formation (PubMed:16430695, PubMed:16430696, PubMed:21477127, PubMed:24488317). Required for the proper anchoring and polymerization of TasA amyloid fibers at the cell surface (PubMed:16430696, PubMed:21477127, PubMed:24488317). Is also a minor component of TasA fibers (PubMed:21477127).<ref>PMID:16430695</ref> <ref>PMID:16430696</ref> <ref>PMID:21477127</ref> <ref>PMID:24488317</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Diehl, A]]
		+	[[Category: Higman, V A]]
		+	[[Category: Oschkinat, H]]
		+	[[Category: Schmieder, P]]
		+	[[Category: Anchoring]]
		+	[[Category: Biofilm]]
		+	[[Category: Protein fibril]]
		+	[[Category: Tapa]]
		+	[[Category: Tasa]]

OCA: Protected "6qay" [edit=sysop:move=sysop]

2018-12-26T07:04:15Z

Protected "6qay" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:04, 26 December 2018

OCA: New page: '''Unreleased structure''' The entry 6qay is ON HOLD Authors: Description: Category: Unreleased Structures

2018-12-26T07:04:13Z

New page: '''Unreleased structure''' The entry 6qay is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qay is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6qay - Revision history

OCA at 17:10, 26 April 2023

OCA at 15:34, 29 January 2020

OCA: Protected "6qay" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6qay is ON HOLD Authors: Description: Category: Unreleased Structures