6qim - Revision history

OCA at 12:01, 24 January 2024

2024-01-24T12:01:46Z

←Older revision		Revision as of 12:01, 24 January 2024
Line 3:		Line 3:
	<StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QIM FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PIP2~~-4, ~~At5g60660, MUP24.9 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=3702 ARATH])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [~~http~~://pdbe.org/6qim PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6qim PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [https://pdbe.org/6qim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [https://www.ebi.ac.uk/pdbsum/6qim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH]] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.	+	[https://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Schoebel, S]]	+	[[Category: Schoebel S]]
-	[[Category: Wang, H]]	+	[[Category: Wang H]]
-	~~[[Category: Aquaporin]]~~	+
-	~~[[Category: Hydrogen peroxide transport]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 12:43, 18 December 2019

2019-12-18T12:43:00Z

←Older revision		Revision as of 12:43, 18 December 2019
Line 3:		Line 3:
	<StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QIM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [http://pdbe.org/6qim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [http://www.ebi.ac.uk/pdbsum/6qim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PIP2-4, At5g60660, MUP24.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [http://pdbe.org/6qim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [http://www.ebi.ac.uk/pdbsum/6qim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH]] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.		[[http://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH]] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aquaporins are membrane-intrinsic proteins initially defined as water (H2O) channels in all organisms and subsequently found to have multiple substrate specificities, such as hydrogen peroxide (H2O2). H2O2 is a signaling molecule that partakes in immune responses where its transport is mediated by aquaporins. To shed further light on the molecular basis of the aquaporin function in H2O2 transport, we have characterized an Arabidopsis thaliana aquaporin, AtPIP2;4, recombinantly produced to high yields in Pichia pastoris. Here, we present a newly established assay that allows detection of H2O2 transport by purified aquaporins reconstituted into liposomes, enabling us to compare aquaporin homologues with respect to substrate specificity. To get additional insight into the structural determinants for aquaporin-mediated H2O2 transport, we solved the 3D-structure of AtPIP2;4 to 3.7A resolution and found structural identity to the water channel from spinach (SoPIP2;1), with the difference that Cd(2+) cation is not required to retain the closed conformation. The transport specificities of the two plant aquaporins were compared to a human homologue, AQP1. Overall, we conclude that AtPIP2;4, SoPIP2;1 and hAQP1 are all transporters of both H2O and H2O2, but have different efficiencies for various specificities. Notably, all three homologues expedite H2O transport equally well while the plant aquaporins are more permeable to H2O2 than hAQP1. Comparison of the structures indicates that the observed variations in H2O and H2O2 transport cannot be explained by differences in the monomeric pore. Possibly, the determinants for transport specificities reside in the flexible domains outside the membrane core of these channels.
		+
		+	Characterization of aquaporin-driven hydrogen peroxide transport.,Wang H, Schoebel S, Schmitz F, Dong H, Hedfalk K Biochim Biophys Acta Biomembr. 2019 Sep 12:183065. doi:, 10.1016/j.bbamem.2019.183065. PMID:31521632<ref>PMID:31521632</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6qim" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Schoebel, S]]		[[Category: Schoebel, S]]

OCA at 15:34, 20 November 2019

2019-11-20T15:34:40Z

←Older revision		Revision as of 15:34, 20 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6qim ~~is ON HOLD~~	+	==Structure of AtPIP2;4==
-		+	<StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution\|resolution]] 3.70Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QIM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [http://pdbe.org/6qim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [http://www.ebi.ac.uk/pdbsum/6qim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH]] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Schoebel, S]]
		+	[[Category: Wang, H]]
		+	[[Category: Aquaporin]]
		+	[[Category: Hydrogen peroxide transport]]
		+	[[Category: Membrane protein]]

OCA: Protected "6qim" [edit=sysop:move=sysop]

2019-01-30T07:00:49Z

Protected "6qim" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:00, 30 January 2019

OCA: New page: '''Unreleased structure''' The entry 6qim is ON HOLD Authors: Description: Category: Unreleased Structures

2019-01-30T07:00:48Z

New page: '''Unreleased structure''' The entry 6qim is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qim is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]