6qoz - Revision history

OCA at 05:04, 11 April 2020

2020-04-11T05:04:33Z

←Older revision		Revision as of 05:04, 11 April 2020
Line 3:		Line 3:
	<SX load='6qoz' size='340' side='right' viewer='molstar' caption='[[6qoz]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6qoz' size='340' side='right' viewer='molstar' caption='[[6qoz]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6qoz]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QOZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6QOZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6qoz]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QOZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QOZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6qoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qoz OCA], [http://pdbe.org/6qoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qoz RCSB], [http://www.ebi.ac.uk/pdbsum/6qoz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qoz ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qoz OCA], [http://pdbe.org/6qoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qoz RCSB], [http://www.ebi.ac.uk/pdbsum/6qoz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qoz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:52, 6 March 2020

2020-03-06T23:52:16Z

←Older revision		Revision as of 23:52, 6 March 2020
Line 1:		Line 1:

	==CryoEM reconstruction of Cowpea Mosaic Virus (CPMV) bound to an Affimer reagent==		==CryoEM reconstruction of Cowpea Mosaic Virus (CPMV) bound to an Affimer reagent==
-	<~~StructureSection~~ load='6qoz' size='340' side='right'caption='[[6qoz]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<SX load='6qoz' size='340' side='right' viewer='molstar' caption='[[6qoz]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qoz]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QOZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qoz]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QOZ FirstGlance]. <br>
Line 20:		Line 20:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Adams, T L]]		[[Category: Adams, T L]]

OCA at 07:50, 18 December 2019

2019-12-18T07:50:32Z

←Older revision		Revision as of 07:50, 18 December 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6qoz is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==CryoEM reconstruction of Cowpea Mosaic Virus (CPMV) bound to an Affimer reagent==
		+	<StructureSection load='6qoz' size='340' side='right'caption='[[6qoz]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6qoz]] is a 9 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QOZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QOZ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qoz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qoz OCA], [http://pdbe.org/6qoz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qoz RCSB], [http://www.ebi.ac.uk/pdbsum/6qoz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qoz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POL2_CPMVS POL2_CPMVS]] Movement protein: transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner.<ref>PMID:10049828</ref> <ref>PMID:15483261</ref> <ref>PMID:15165817</ref> <ref>PMID:14722313</ref> The cleavable C-terminus of small coat protein seems to be involved in the packaging of the virion RNAs. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.<ref>PMID:10049828</ref> <ref>PMID:15483261</ref> <ref>PMID:15165817</ref> <ref>PMID:14722313</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant viruses can cause devastating losses to agriculture and are therefore a major threat to food security. The rapid identification of virally-infected crops allowing containment is essential to limit such threats, but plant viral diseases can be extremely challenging to diagnose. An ideal method for plant virus diagnosis would be a device which can be implemented easily in the field. Such devices require a binding reagent that is specific for the virus of interest. We chose to investigate the use of Affimer reagents, artificial binding proteins and a model plant virus Cowpea Mosaic virus (CPMV) empty virus like particles (eVLPs). CPMV-eVLP mimic the morphology of wild-type (WT) CPMV but lack any infectious genomic material and so do not have biocontainment issues. We have produced and purified an Affimer reagent selected for its ability to bind to CPMV-eVLP and have shown that the selected Affimer also specifically binds to WT CPMV. We have produced a 3.4 A structure of WT CPMV bound to the Affimer using cryo-electron microscopy. Finally, we have shown that this Affimer is capable of reliably detecting the virus in crude extracts of CPMV-infected leaves and can therefore form the basis for the future development of diagnostic tests.

-	~~Authors:~~ Hesketh~~, E.L.~~, Tiede, C., Adamson, H., Adams~~, T.L.~~, Byrne~~, M.J.~~, Meshcheriakova, Y~~., Lomonossoff, G.P.~~, Kruse, I., McPherson, ~~M.J.~~, Tomlinson, D.C.~~, Ranson, N~~.A.	+	Affimer reagents as tools in diagnosing plant virus diseases.,Hesketh EL, Tiede C, Adamson H, Adams TL, Byrne MJ, Meshcheriakova Y, Kruse I, McPherson MJ, Lomonossoff GP, Tomlinson DC, Ranson NA Sci Rep. 2019 May 17;9(1):7524. doi: 10.1038/s41598-019-43945-6. PMID:31101847<ref>PMID:31101847</ref>

-	~~Description: CryoEM reconstructuion~~ of ~~Cowpea Mosaic Virus (CPMV) bound to an Affimer reagent~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Adams, T~~.~~L]]~~	+	<div class="pdbe-citations 6qoz" style="background-color:#fffaf0;"></div>
-	~~[[Category: Mcpherson, M~~.~~J]]~~	+	== References ==
-	~~[[Category: Hesketh, E~~.~~L]]~~	+	<references/>
-	~~[[Category: Tiede, C]]~~	+	__TOC__
-	~~[[Category~~: ~~Ranson, N.A]]~~	+	</StructureSection>
-	~~[[Category: Kruse, I]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Tomlinson, D.C]]~~	+	[[Category: Adams, T L]]
-	[[Category: ~~Byrne, M.J~~]]	+
-	[[Category: ~~Lomonossoff~~, ~~G.P~~]]	+
	[[Category: Adamson, H]]		[[Category: Adamson, H]]
		+	[[Category: Byrne, M J]]
		+	[[Category: Hesketh, E L]]
		+	[[Category: Kruse, I]]
		+	[[Category: Lomonossoff, G P]]
		+	[[Category: McPherson, M J]]
	[[Category: Meshcheriakova, Y]]		[[Category: Meshcheriakova, Y]]
		+	[[Category: Ranson, N A]]
		+	[[Category: Tiede, C]]
		+	[[Category: Tomlinson, D C]]
		+	[[Category: Affimer reagent]]
		+	[[Category: Antibody-like]]
		+	[[Category: Comoviridae]]
		+	[[Category: Cpmv]]
		+	[[Category: Picornavirale]]
		+	[[Category: Virus]]

OCA at 07:19, 2 October 2019

2019-10-02T07:19:28Z

←Older revision		Revision as of 07:19, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6qoz is ON HOLD	+	The entry 6qoz is ON HOLD until Paper Publication

	Authors: Hesketh, E.L., Tiede, C., Adamson, H., Adams, T.L., Byrne, M.J., Meshcheriakova, Y., Lomonossoff, G.P., Kruse, I., McPherson, M.J., Tomlinson, D.C., Ranson, N.A.		Authors: Hesketh, E.L., Tiede, C., Adamson, H., Adams, T.L., Byrne, M.J., Meshcheriakova, Y., Lomonossoff, G.P., Kruse, I., McPherson, M.J., Tomlinson, D.C., Ranson, N.A.

OCA: Protected "6qoz" [edit=sysop:move=sysop]

2019-02-21T05:25:24Z

Protected "6qoz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 21 February 2019

OCA: New page: '''Unreleased structure''' The entry 6qoz is ON HOLD Authors: Hesketh, E.L., Tiede, C., Adamson, H., Adams, T.L., Byrne, M.J., Meshcheriakova, Y., Lomonossoff, G.P., Kruse, I., McPherso...

2019-02-21T05:25:23Z

New page: '''Unreleased structure''' The entry 6qoz is ON HOLD Authors: Hesketh, E.L., Tiede, C., Adamson, H., Adams, T.L., Byrne, M.J., Meshcheriakova, Y., Lomonossoff, G.P., Kruse, I., McPherso...

New page

'''Unreleased structure'''

The entry 6qoz is ON HOLD

Authors: Hesketh, E.L., Tiede, C., Adamson, H., Adams, T.L., Byrne, M.J., Meshcheriakova, Y., Lomonossoff, G.P., Kruse, I., McPherson, M.J., Tomlinson, D.C., Ranson, N.A.

Description: CryoEM reconstructuion of Cowpea Mosaic Virus (CPMV) bound to an Affimer reagent
[[Category: Unreleased Structures]]
[[Category: Adams, T.L]]
[[Category: Mcpherson, M.J]]
[[Category: Hesketh, E.L]]
[[Category: Tiede, C]]
[[Category: Ranson, N.A]]
[[Category: Kruse, I]]
[[Category: Tomlinson, D.C]]
[[Category: Byrne, M.J]]
[[Category: Lomonossoff, G.P]]
[[Category: Adamson, H]]
[[Category: Meshcheriakova, Y]]