6r5s - Revision history

OCA at 12:15, 24 January 2024

2024-01-24T12:15:47Z

←Older revision		Revision as of 12:15, 24 January 2024
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	<StructureSection load='6r5s' size='340' side='right'caption='[[6r5s]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='6r5s' size='340' side='right'caption='[[6r5s]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r5s]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6R5S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R5S FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class="sblockDat">[[6r3z\|6r3z]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [https://pdbe.org/6r5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [https://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">psaA, C0044_17990, CAZ10_34375, DZ962_30765, NCTC13719_02701, PAMH19_5875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [~~http~~://pdbe.org/6r5s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I174_PSEAE Q9I174_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Morera, S~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Vigouroux, A~~]]	+	[[Category: Morera S]]
-	[[Category: ~~Transport protein~~]]	+	[[Category: Vigouroux A]]

OCA at 16:17, 29 January 2020

2020-01-29T16:17:45Z

←Older revision		Revision as of 16:17, 29 January 2020
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	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6r3z\|6r3z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6r3z\|6r3z]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~EIM05_28735~~ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">psaA, C0044_17990, CAZ10_34375, DZ962_30765, NCTC13719_02701, PAMH19_5875 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [http://pdbe.org/6r5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [http://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [http://pdbe.org/6r5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [http://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:25, 7 August 2019

2019-08-07T06:25:09Z

←Older revision		Revision as of 06:25, 7 August 2019
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	<StructureSection load='6r5s' size='340' side='right'caption='[[6r5s]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='6r5s' size='340' side='right'caption='[[6r5s]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r5s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R5S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r5s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R5S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6r3z\|6r3z]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6r3z\|6r3z]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EIM05_28735 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [http://pdbe.org/6r5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [http://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [http://pdbe.org/6r5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [http://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:31, 31 July 2019

2019-07-31T06:31:13Z

←Older revision		Revision as of 06:31, 31 July 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6r5s is ~~ON HOLD until Paper~~ Publication	+	==Structure of the SBP FpvC from pseudomonas aeruginosa in complex with Fe(II)==
		+	<StructureSection load='6r5s' size='340' side='right'caption='[[6r5s]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6r5s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R5S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6r3z\|6r3z]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r5s OCA], [http://pdbe.org/6r5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r5s RCSB], [http://www.ebi.ac.uk/pdbsum/6r5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r5s ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosa secretes pyoverdine, a major siderophore to get access to iron, an essential nutrient. Pyoverdine scavenges ferric iron in the bacterial environment with the resulting complex internalized by bacteria. Releasing of iron from pyoverdine in the periplasm involves an iron reduction by an inner membrane reductase and two solute-binding proteins (SBPs) FpvC and FpvF in association with their ABC transporter. FpvC and FpvF belong to two different subgroups of SBPs within the structural cluster A: FpvC and FpvF were proposed to be a metal-binding protein and a ferrisiderophore-binding protein respectively. Here, we report the redox state and the binding mode of iron to FpvC. We first solved the crystal structure of FpvC bound to a fortuitous Ni(2+) by single anomalous dispersion method. Using a different protein purification strategy, we determined the structure of FpvC with manganese and iron, which binds to FpvC in a ferrous state as demonstrated by electron paramagnetic resonance. FpvC is the first example of a hexahistidine metal site among SBPs in which the Fe(2+) redox state is stabilized under aerobic conditions. Using biophysics methods, we showed that FpvC reversibly bind to a broad range of divalent ions. The structure of a mutant mimicking the apo FpvC reveals a protein in an open state with large conformational changes when compared with the metal-bound FpvC. These results highlight that the canonical metal site in FpvC is distinct from those yet described in SBPs and they provide new insights into the mechanism of PVD-Fe dissociation in P. aeruginosa.

-	~~Authors~~:	+	A unique ferrous iron binding mode is associated with large conformational changes for the transport protein FpvC of Pseudomonas aeruginosa.,Vigouroux A, Aumont-Nicaise M, Boussac A, Marty L, Lo Bello L, Legrand P, Brillet K, Schalk IJ, Morera S FEBS J. 2019 Jul 18. doi: 10.1111/febs.15004. PMID:31318478<ref>PMID:31318478</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6r5s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Morera, S]]
		+	[[Category: Vigouroux, A]]
		+	[[Category: Transport protein]]

OCA at 05:38, 10 April 2019

2019-04-10T05:38:41Z

←Older revision		Revision as of 05:38, 10 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6r5s is ON HOLD	+	The entry 6r5s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6r5s" [edit=sysop:move=sysop]

2019-04-03T05:00:12Z

Protected "6r5s" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:00, 3 April 2019

OCA: New page: '''Unreleased structure''' The entry 6r5s is ON HOLD Authors: Description: Category: Unreleased Structures

2019-04-03T05:00:10Z

New page: '''Unreleased structure''' The entry 6r5s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6r5s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]