6r69 - Revision history

OCA at 05:18, 11 April 2020

2020-04-11T05:18:07Z

←Older revision		Revision as of 05:18, 11 April 2020
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	<SX load='6r69' size='340' side='right' viewer='molstar' caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>		<SX load='6r69' size='340' side='right' viewer='molstar' caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6R69 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6R69 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f2d\|6f2d]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f2d\|6f2d]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fliP, LTSERUB_0568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliR, flaP, STM1981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliQ, fliQ_1, fliQ_2, A7D45_07890, A7S51_18675, AL463_17545, BH006_19970, BUJ17_0011480, BUJ19_005435, C4801_20645, C4860_05670, CBI64_04070, CHC34_11945, CHD23_08750, CHE19_09990, CIC26_24535, CRE05_10505, IN95_15645, NCTC10252_03215, NCTC10718_02756, NCTC6385_02264, NCTC9854_01819 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fliP, LTSERUB_0568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliR, flaP, STM1981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliQ, fliQ_1, fliQ_2, A7D45_07890, A7S51_18675, AL463_17545, BH006_19970, BUJ17_0011480, BUJ19_005435, C4801_20645, C4860_05670, CBI64_04070, CHC34_11945, CHD23_08750, CHE19_09990, CIC26_24535, CRE05_10505, IN95_15645, NCTC10252_03215, NCTC10718_02756, NCTC6385_02264, NCTC9854_01819 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6r69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r69 OCA], [http://pdbe.org/6r69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r69 RCSB], [http://www.ebi.ac.uk/pdbsum/6r69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r69 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6r69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r69 OCA], [http://pdbe.org/6r69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r69 RCSB], [http://www.ebi.ac.uk/pdbsum/6r69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r69 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 23:59, 6 March 2020

2020-03-06T23:59:39Z

←Older revision		Revision as of 23:59, 6 March 2020
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	==Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.==		==Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.==
-	<~~StructureSection~~ load='6r69' size='340' side='right'caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>	+	<SX load='6r69' size='340' side='right' viewer='molstar' caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R69 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R69 FirstGlance]. <br>
Line 25:		Line 25:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bacillus cholerae-suis smith 1894]]		[[Category: Bacillus cholerae-suis smith 1894]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:40, 10 July 2019

2019-07-10T06:40:47Z

←Older revision		Revision as of 06:40, 10 July 2019
Line 3:		Line 3:
	<StructureSection load='6r69' size='340' side='right'caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>		<StructureSection load='6r69' size='340' side='right'caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R69 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R69 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f2d\|6f2d]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f2d\|6f2d]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fliP, LTSERUB_0568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliR, flaP, STM1981 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894]), fliQ, fliQ_1, fliQ_2, A7D45_07890, A7S51_18675, AL463_17545, BH006_19970, BUJ17_0011480, BUJ19_005435, C4801_20645, C4860_05670, CBI64_04070, CHC34_11945, CHD23_08750, CHE19_09990, CIC26_24535, CRE05_10505, IN95_15645, NCTC10252_03215, NCTC10718_02756, NCTC6385_02264, NCTC9854_01819 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 "Bacillus cholerae-suis" Smith 1894])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r69 OCA], [http://pdbe.org/6r69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r69 RCSB], [http://www.ebi.ac.uk/pdbsum/6r69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r69 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r69 OCA], [http://pdbe.org/6r69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r69 RCSB], [http://www.ebi.ac.uk/pdbsum/6r69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r69 ProSAT]</span></td></tr>
	</table>		</table>
Line 11:		Line 12:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. ~~Three~~ putative ~~integral~~ membrane proteins (~~FliP~~, ~~FliQ, FliR~~) ~~are suggested to form the core of~~ an ~~export gate in the inner membrane, but their structure,~~ assembly ~~and location within~~ the ~~final nanomachine remain unclear~~. Here, we present the ~~cryoelectron microscopy~~ structure of the ~~Salmonella Typhimurium FliP-FliQ-FliR complex~~ at 4.2 A. ~~None~~ of the ~~subunits adopt canonical integral membrane protein topologies,~~ and ~~common helix-turn-helix~~ structural ~~elements allow them to form a helical assembly with 5:4:1 stoichiometry~~. ~~Fitting~~ of the ~~structure into reconstructions~~ of ~~intact~~ secretion systems, ~~combined with cross-linking~~, ~~localize~~ the ~~export gate as a core component of~~ the ~~periplasmic portion~~ of the ~~machinery~~. This ~~study thereby identifies~~ the export gate ~~as a key element~~ of ~~the secretion channel and implies that it primes the helical architecture~~ of ~~the components assembling downstream~~.	+	Export of proteins through type III secretion systems (T3SS) is critical for motility and virulence of many major bacterial pathogens. Proteins are exported through a genetically defined export gate complex consisting of three proteins. We have recently shown at 4.2 A that the flagellar complex of these three putative membrane proteins (FliPQR in flagellar systems, SctRST in virulence systems) assembles into an extramembrane helical assembly that likely seeds correct assembly of the rod. Here we present the structure of an equivalent complex from the Shigella virulence system at 3.5 A by cryo-electron microscopy. This higher-resolution structure yields a more precise description of the structure and confirms the prediction of structural conservation in this core complex. Analysis of particle heterogeneity also suggests how the SctS/FliQ subunits sequentially assemble in the complex.IMPORTANCE Although predicted on the basis of sequence conservation, the work presented here formally demonstrates that all classes of type III secretion systems, flagellar or virulence, share the same architecture at the level of the core structures. This absolute conservation of the unusual extramembrane structure of the core export gate complex now allows work to move to focusing on both mechanistic studies of type III but also on fundamental studies of how such a complex is assembled.

-	Structure of ~~the core~~ of the ~~type~~ III ~~secretion system export apparatus~~.~~,Kuhlen L, Abrusci P~~, Johnson S, ~~Gault J~~, Deme J, ~~Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV~~, Lea SM ~~Nat Struct Mol Biol~~. ~~2018 Jul;~~25(7):~~583~~-~~590~~. doi: 10.~~1038~~/~~s41594~~-~~018-0086-9. Epub, 2018 Jul 2~~. PMID:~~29967543~~<ref>PMID:~~29967543~~</ref>	+	The Structure of an Injectisome Export Gate Demonstrates Conservation of Architecture in the Core Export Gate between Flagellar and Virulence Type III Secretion Systems.,Johnson S, Kuhlen L, Deme JC, Abrusci P, Lea SM MBio. 2019 Jun 25;10(3). pii: mBio.00818-19. doi: 10.1128/mBio.00818-19. PMID:31239376<ref>PMID:31239376</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	<div class="pdbe-citations 6r69" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6r69" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flagellar protein 3D structures\|Flagellar protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus cholerae-suis smith 1894]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abrusci, P]]		[[Category: Abrusci, P]]

OCA at 06:22, 29 May 2019

2019-05-29T06:22:39Z

←Older revision		Revision as of 06:22, 29 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6r69 is ~~ON HOLD until Paper~~ Publication	+	==Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.==
		+	<StructureSection load='6r69' size='340' side='right'caption='[[6r69]], [[Resolution\|resolution]] 3.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6r69]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R69 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f2d\|6f2d]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r69 OCA], [http://pdbe.org/6r69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r69 RCSB], [http://www.ebi.ac.uk/pdbsum/6r69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r69 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/G5QE81_SALRU G5QE81_SALRU]] Plays a role in the flagellum-specific transport system.[RuleBase:RU362069] [[http://www.uniprot.org/uniprot/A0A0M0QTK6_SALER A0A0M0QTK6_SALER]] Role in flagellar biosynthesis.[RuleBase:RU364090] [[http://www.uniprot.org/uniprot/FLIR_SALTY FLIR_SALTY]] Role in flagellar biosynthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 A. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream.

-	~~Authors: Johnson, S~~., Kuhlen, L., Abrusci, P., ~~Lea~~, S.M.	+	Structure of the core of the type III secretion system export apparatus.,Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543<ref>PMID:29967543</ref>

-	~~Description: Improved map~~ of the ~~FliPQR complex that forms the core~~ of ~~the Salmonella type III secretion system export apparatus~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Kuhlen, L~~]]	+	<div class="pdbe-citations 6r69" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Abrusci, P]]		[[Category: Abrusci, P]]
-	[[Category: Lea, S.M]]
	[[Category: Johnson, S]]		[[Category: Johnson, S]]
		+	[[Category: Kuhlen, L]]
		+	[[Category: Lea, S M]]
		+	[[Category: Cryo-em]]
		+	[[Category: Export]]
		+	[[Category: Flagella]]
		+	[[Category: Membrane protein]]
		+	[[Category: Protein transport]]
		+	[[Category: T3ss]]

OCA at 04:12, 22 May 2019

2019-05-22T04:12:40Z

←Older revision		Revision as of 04:12, 22 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6r69 is ON HOLD	+	The entry 6r69 is ON HOLD until Paper Publication

	Authors: Johnson, S., Kuhlen, L., Abrusci, P., Lea, S.M.		Authors: Johnson, S., Kuhlen, L., Abrusci, P., Lea, S.M.
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	Description: Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.		Description: Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Johnson, S]]
	[[Category: Kuhlen, L]]		[[Category: Kuhlen, L]]
	[[Category: Abrusci, P]]		[[Category: Abrusci, P]]
	[[Category: Lea, S.M]]		[[Category: Lea, S.M]]
		+	[[Category: Johnson, S]]

OCA: Protected "6r69" [edit=sysop:move=sysop]

2019-04-03T05:02:04Z

Protected "6r69" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 3 April 2019

OCA: New page: '''Unreleased structure''' The entry 6r69 is ON HOLD Authors: Johnson, S., Kuhlen, L., Abrusci, P., Lea, S.M. Description: Improved map of the FliPQR complex that forms the core of the...

2019-04-03T05:02:02Z

New page: '''Unreleased structure''' The entry 6r69 is ON HOLD Authors: Johnson, S., Kuhlen, L., Abrusci, P., Lea, S.M. Description: Improved map of the FliPQR complex that forms the core of the...

New page

'''Unreleased structure'''

The entry 6r69 is ON HOLD

Authors: Johnson, S., Kuhlen, L., Abrusci, P., Lea, S.M.

Description: Improved map of the FliPQR complex that forms the core of the Salmonella type III secretion system export apparatus.
[[Category: Unreleased Structures]]
[[Category: Johnson, S]]
[[Category: Kuhlen, L]]
[[Category: Abrusci, P]]
[[Category: Lea, S.M]]