6s0m - Revision history

OCA at 05:51, 13 May 2020

2020-05-13T05:51:37Z

←Older revision		Revision as of 05:51, 13 May 2020
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	<StructureSection load='6s0m' size='340' side='right'caption='[[6s0m]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6s0m' size='340' side='right'caption='[[6s0m]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s0m]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0M OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6S0M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s0m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S0M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6s0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0m OCA], [http://pdbe.org/6s0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s0m RCSB], [http://www.ebi.ac.uk/pdbsum/6s0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0m ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DSI35_27935, ERS007661_00730, ERS007663_02388, ERS007665_04230, ERS007703_00653, ERS007722_01383, ERS007741_00970, ERS023446_01558, ERS027644_03090, ERS027651_00179, ERS027652_01481, ERS027653_00165, ERS027654_01704, ERS027656_02031, ERS027661_01114, ERS027666_02618, ERS031537_02141, ERS124361_01165, SAMEA2682864_00498, SAMEA2683035_01322 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0m OCA], [http://pdbe.org/6s0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s0m RCSB], [http://www.ebi.ac.uk/pdbsum/6s0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 15:43, 28 August 2019

2019-08-28T15:43:21Z

←Older revision		Revision as of 15:43, 28 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6s0m is ~~ON HOLD~~	+	==Structural and dynamic studies provide insights into specificity and allosteric regulation of Ribonuclease AS, a key enzyme in mycobacterial virulence==
		+	<StructureSection load='6s0m' size='340' side='right'caption='[[6s0m]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s0m]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S0M FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0m OCA], [http://pdbe.org/6s0m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s0m RCSB], [http://www.ebi.ac.uk/pdbsum/6s0m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0m ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A045JQ63_MYCTX A0A045JQ63_MYCTX]] Exonuclease that cleaves single-stranded 3' overhangs of double-stranded RNA.[HAMAP-Rule:MF_00977]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribonuclease AS (RNase AS) is a crucial enzyme for virulence of Mycobacterium tuberculosis. We previously observed that RNase AS structurally resembles RNase T from Escherichia coli, an important enzyme for tRNA maturation and turnover. Here, we combine X-ray crystallography and molecular dynamics (MD) to investigate the specificity and dynamic properties of substrate binding. Both X-ray and MD data provide structural determinants that corroborate the strict substrate specificity of RNase AS to cleave only adenosine residues, due to the structural features of adenine base. Beside suggesting tRNA as most likely substrate of RNase AS, MD and modeling studies identify key enzyme-ligand interactions, both involving the catalytic site and the double helix region of tRNA, which is locked by interactions with a set of arginine residues. The MD data also evidence a ligand-induced conformational change of the enzyme which is transferred from one chain to the adjacent one. These data will explain the dimeric nature of both RNase AS and RNase T, with two catalytic grooves composed of both chains. Also, they account for the dichotomy of tRNA, which contains both the substrate poly(A) chain and an inhibiting double strand RNA. Indeed, they provide a possible mechanism of allosteric regulation, which unlocks one catalytic groove when the second groove is inhibited by the double strand region of tRNA. Finally, a full comprehension of the molecular details of tRNA maturation processes is essential to develop novel strategies to modulate RNA processing, for therapeutic purposes. Abbreviations MD molecular dynamics PDB Protein Data Bank RMSD root mean square deviation RMSF root mean square fluctuation RNA ribonucleotidic acid RNase AS Ribonuclease AS Communicated by Ramasamy H. Sarma.

-	~~Authors: Berisio~~, R., ~~Romano~~, M.	+	Structural and dynamic studies provide insights into specificity and allosteric regulation of ribonuclease as, a key enzyme in mycobacterial virulence.,Calvanese L, Squeglia F, Romano M, D'Auria G, Falcigno L, Berisio R J Biomol Struct Dyn. 2019 Jul 22:1-13. doi: 10.1080/07391102.2019.1643786. PMID:31299874<ref>PMID:31299874</ref>

-	~~Description: Structural and dynamic studies provide insights into specificity and allosteric regulation of Ribonuclease AS~~, a ~~key enzyme in mycobacterial virulence~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Romano, M~~]]	+	<div class="pdbe-citations 6s0m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Berisio, R]]		[[Category: Berisio, R]]
		+	[[Category: Romano, M]]
		+	[[Category: Rna binding protein]]

OCA: Protected "6s0m" [edit=sysop:move=sysop]

2019-06-26T05:27:20Z

Protected "6s0m" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6s0m is ON HOLD Authors: Berisio, R., Romano, M. Description: Structural and dynamic studies provide insights into specificity and allosteric regul...

2019-06-26T05:27:18Z

New page: '''Unreleased structure''' The entry 6s0m is ON HOLD Authors: Berisio, R., Romano, M. Description: Structural and dynamic studies provide insights into specificity and allosteric regul...

New page

'''Unreleased structure'''

The entry 6s0m is ON HOLD

Authors: Berisio, R., Romano, M.

Description: Structural and dynamic studies provide insights into specificity and allosteric regulation of Ribonuclease AS, a key enzyme in mycobacterial virulence
[[Category: Unreleased Structures]]
[[Category: Romano, M]]
[[Category: Berisio, R]]