6s0q - Revision history

OCA at 16:05, 13 December 2023

2023-12-13T16:05:47Z

←Older revision		Revision as of 16:05, 13 December 2023
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	==Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns==		==Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns==
-	<StructureSection load='6s0q' size='340' side='right'caption='[[6s0q]]' scene=''>	+	<StructureSection load='6s0q' size='340' side='right'caption='[[6s0q]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6S0Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S0Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6s0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0q OCA], [~~http~~://pdbe.org/6s0q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6s0q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6s0q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0q ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=OLA:OLEIC+ACID'>OLA</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=ZMA:4-{2-[(7-AMINO-2-FURAN-2-YL[1,2,4]TRIAZOLO[1,5-A][1,3,5]TRIAZIN-5-YL)AMINO]ETHYL}PHENOL'>ZMA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0q OCA], [https://pdbe.org/6s0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s0q RCSB], [https://www.ebi.ac.uk/pdbsum/6s0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AA2AR_HUMAN AA2AR_HUMAN] Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.[https://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX] Electron-transport protein of unknown function.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Long-wavelength pulses from the Swiss X-ray free-electron laser (XFEL) have been used for de novo protein structure determination by native single-wavelength anomalous diffraction (native-SAD) phasing of serial femtosecond crystallography (SFX) data. In this work, sensitive anomalous data-quality indicators and model proteins were used to quantify improvements in native-SAD at XFELs such as utilization of longer wavelengths, careful experimental geometry optimization, and better post-refinement and partiality correction. Compared with studies using shorter wavelengths at other XFELs and older software versions, up to one order of magnitude reduction in the required number of indexed images for native-SAD was achieved, hence lowering sample consumption and beam-time requirements significantly. Improved data quality and higher anomalous signal facilitate so-far underutilized de novo structure determination of challenging proteins at XFELs. Improvements presented in this work can be used in other types of SFX experiments that require accurate measurements of weak signals, for example time-resolved studies.
		+
		+	Advances in long-wavelength native phasing at X-ray free-electron lasers.,Nass K, Cheng R, Vera L, Mozzanica A, Redford S, Ozerov D, Basu S, James D, Knopp G, Cirelli C, Martiel I, Casadei C, Weinert T, Nogly P, Skopintsev P, Usov I, Leonarski F, Geng T, Rappas M, Dore AS, Cooke R, Nasrollahi Shirazi S, Dworkowski F, Sharpe M, Olieric N, Bacellar C, Bohinc R, Steinmetz MO, Schertler G, Abela R, Patthey L, Schmitt B, Hennig M, Standfuss J, Wang M, Milne CJ IUCrJ. 2020 Sep 9;7(Pt 6):965-975. doi: 10.1107/S2052252520011379. eCollection, 2020 Nov 1. PMID:33209311<ref>PMID:33209311</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6s0q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adenosine A2A receptor 3D structures\|Adenosine A2A receptor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abela R]]		[[Category: Abela R]]

OCA at 08:07, 2 December 2020

2020-12-02T08:07:37Z

←Older revision		Revision as of 08:07, 2 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6s0q ~~is ON HOLD~~	+	==Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns==
-		+	<StructureSection load='6s0q' size='340' side='right'caption='[[6s0q]]' scene=''>
-	~~Authors~~: ~~Nass, K~~.~~, Cheng, R~~.~~, Vera, L~~.~~, Mozzanica, A~~.~~, Redford, S~~.~~, Ozerov, D~~.~~, Basu, S~~.~~, James, D~~.~~, Knopp, G~~., ~~Cirelli, C~~.~~, Martiel, I~~.~~, Casadei, C~~., ~~Weinert, T~~., ~~Nogly, P~~.~~, Skopintsev, P~~.~~, Usov, I~~., ~~Leonarski, F~~.~~, Geng, T~~.~~, Rappas, M~~., ~~Dore, A~~.~~S., Cooke, R., Nasrollahi Shirazi, S., Dworkowski, F., Sharpe, M., Olieric, N., Steinmetz, M.O., Schertler, G., Abela, R., Patthey, L., Schmitt, B., Hennig, M., Standfuss, J., Wang, M., Milne, J.C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6S0Q FirstGlance]. <br>
-	~~Description: Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6s0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0q OCA], [http://pdbe.org/6s0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s0q RCSB], [http://www.ebi.ac.uk/pdbsum/6s0q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wang, M~~]]	+	__TOC__
-	[[Category: ~~Skopintsev, P~~]]	+	</StructureSection>
-	[[Category: ~~Abela, R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Patthey, L~~]]	+	[[Category: Abela R]]
-	[[Category: ~~Schmitt, B~~]]	+	[[Category: Basu S]]
-	[[Category: ~~Olieric, N~~]]	+	[[Category: Casadei C]]
-	[[Category: ~~Usov, I~~]]	+	[[Category: Cheng R]]
-	[[Category: ~~Cheng, R~~]]	+	[[Category: Cirelli C]]
-	[[Category: ~~Casadei, C~~]]	+	[[Category: Cooke R]]
-	[[Category: ~~Nass, K~~]]	+	[[Category: Dore AS]]
-	[[Category: ~~Redford, S~~]]	+	[[Category: Dworkowski F]]
-	[[Category: ~~Cooke, R~~]]	+	[[Category: Geng T]]
-	[[Category: ~~Knopp, G~~]]	+	[[Category: Hennig M]]
-	[[Category: ~~Hennig, M~~]]	+	[[Category: James D]]
-	[[Category: ~~Steinmetz, M.O~~]]	+	[[Category: Knopp G]]
-	[[Category: ~~Martiel, I~~]]	+	[[Category: Leonarski F]]
-	[[Category: ~~Vera, L~~]]	+	[[Category: Martiel I]]
-	[[Category: ~~Dore, A.S~~]]	+	[[Category: Milne JC]]
-	[[Category: Nogly, P]]	+	[[Category: Mozzanica A]]
-	[[Category: ~~Dworkowski, F~~]]	+	[[Category: Nasrollahi Shirazi S]]
-	[[Category: ~~Schertler, G~~]]	+	[[Category: Nass K]]
-	[[Category: ~~Mozzanica, A~~]]	+	[[Category: Nogly P]]
-	[[Category: ~~Ozerov, D~~]]	+	[[Category: Olieric N]]
-	[[Category: ~~Geng, T~~]]	+	[[Category: Ozerov D]]
-	[[Category: ~~James, D~~]]	+	[[Category: Patthey L]]
-	[[Category: ~~Nasrollahi Shirazi, S~~]]	+	[[Category: Rappas M]]
-	[[Category: ~~Weinert, T~~]]	+	[[Category: Redford S]]
-	[[Category: ~~Standfuss, J~~]]	+	[[Category: Schertler G]]
-	[[Category: ~~Basu, S~~]]	+	[[Category: Schmitt B]]
-	[[Category: ~~Cirelli, C~~]]	+	[[Category: Sharpe M]]
-	[[Category: ~~Milne, J.C~~]]	+	[[Category: Skopintsev P]]
-	[[Category: ~~Sharpe, M~~]]	+	[[Category: Standfuss J]]
-	[[Category: ~~Rappas,~~ M]]	+	[[Category: Steinmetz MO]]
-	[[Category: ~~Leonarski, F~~]]	+	[[Category: Usov I]]
		+	[[Category: Vera L]]
		+	[[Category: Wang M]]
		+	[[Category: Weinert T]]

OCA: Protected "6s0q" [edit=sysop:move=sysop]

2019-06-26T05:27:36Z

Protected "6s0q" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 26 June 2019

OCA: New page: '''Unreleased structure''' The entry 6s0q is ON HOLD Authors: Nass, K., Cheng, R., Vera, L., Mozzanica, A., Redford, S., Ozerov, D., Basu, S., James, D., Knopp, G., Cirelli, C., Martiel...

2019-06-26T05:27:35Z

New page: '''Unreleased structure''' The entry 6s0q is ON HOLD Authors: Nass, K., Cheng, R., Vera, L., Mozzanica, A., Redford, S., Ozerov, D., Basu, S., James, D., Knopp, G., Cirelli, C., Martiel...

New page

'''Unreleased structure'''

The entry 6s0q is ON HOLD

Authors: Nass, K., Cheng, R., Vera, L., Mozzanica, A., Redford, S., Ozerov, D., Basu, S., James, D., Knopp, G., Cirelli, C., Martiel, I., Casadei, C., Weinert, T., Nogly, P., Skopintsev, P., Usov, I., Leonarski, F., Geng, T., Rappas, M., Dore, A.S., Cooke, R., Nasrollahi Shirazi, S., Dworkowski, F., Sharpe, M., Olieric, N., Steinmetz, M.O., Schertler, G., Abela, R., Patthey, L., Schmitt, B., Hennig, M., Standfuss, J., Wang, M., Milne, J.C.

Description: Structure of the A2A adenosine receptor determined at SwissFEL using native-SAD at 4.57 keV from 50,000 diffraction patterns
[[Category: Unreleased Structures]]
[[Category: Wang, M]]
[[Category: Skopintsev, P]]
[[Category: Abela, R]]
[[Category: Patthey, L]]
[[Category: Schmitt, B]]
[[Category: Olieric, N]]
[[Category: Usov, I]]
[[Category: Cheng, R]]
[[Category: Casadei, C]]
[[Category: Nass, K]]
[[Category: Redford, S]]
[[Category: Cooke, R]]
[[Category: Knopp, G]]
[[Category: Hennig, M]]
[[Category: Steinmetz, M.O]]
[[Category: Martiel, I]]
[[Category: Vera, L]]
[[Category: Dore, A.S]]
[[Category: Nogly, P]]
[[Category: Dworkowski, F]]
[[Category: Schertler, G]]
[[Category: Mozzanica, A]]
[[Category: Ozerov, D]]
[[Category: Geng, T]]
[[Category: James, D]]
[[Category: Nasrollahi Shirazi, S]]
[[Category: Weinert, T]]
[[Category: Standfuss, J]]
[[Category: Basu, S]]
[[Category: Cirelli, C]]
[[Category: Milne, J.C]]
[[Category: Sharpe, M]]
[[Category: Rappas, M]]
[[Category: Leonarski, F]]