6s31 - Revision history

OCA at 07:32, 19 February 2020

2020-02-19T07:32:04Z

←Older revision		Revision as of 07:32, 19 February 2020
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	<StructureSection load='6s31' size='340' side='right'caption='[[6s31]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='6s31' size='340' side='right'caption='[[6s31]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s31]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S31 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s31]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Galsu Galsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S31 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HBA:P-HYDROXYBENZALDEHYDE'>HBA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HBA:P-HYDROXYBENZALDEHYDE'>HBA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gasu_54250 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=130081 GALSU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s31 OCA], [http://pdbe.org/6s31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s31 RCSB], [http://www.ebi.ac.uk/pdbsum/6s31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s31 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s31 OCA], [http://pdbe.org/6s31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s31 RCSB], [http://www.ebi.ac.uk/pdbsum/6s31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s31 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Galsu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bergantino, E]]		[[Category: Bergantino, E]]

OCA at 15:37, 29 January 2020

2020-01-29T15:37:48Z

←Older revision		Revision as of 15:37, 29 January 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6s31 is ~~ON HOLD~~	+	==Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria in complex with 4-Hydroxybenzaldehyde==
		+	<StructureSection load='6s31' size='340' side='right'caption='[[6s31]], [[Resolution\|resolution]] 1.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s31]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S31 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HBA:P-HYDROXYBENZALDEHYDE'>HBA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s31 OCA], [http://pdbe.org/6s31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s31 RCSB], [http://www.ebi.ac.uk/pdbsum/6s31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s31 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Looking for new ene-reductases with uncovered features beneficial for biotechnological applications, by mining genomes of photosynthetic extremophile organisms, we identified two new Old Yellow Enzyme homologues: CtOYE, deriving from the cyanobacterium Chroococcidiopsis thermalis, and GsOYE, from the alga Galdieria sulphuraria. Both enzymes were produced and purified with very good yields and displayed catalytic activity on a broad substrate spectrum by reducing alpha,beta-unsaturated ketones, aldehydes, maleimides and nitroalkenes with good to excellent stereoselectivity. Both enzymes prefer NADPH but demonstrate a good acceptance of NADH as cofactor. CtOYE and GsOYE represent robust biocatalysts showing high thermostability, a wide range of pH optimum and good co-solvent tolerance. High resolution X-ray crystal structures of both enzymes have been determined, revealing conserved features of the classical OYE subfamily as well as unique properties, such as a very long loop entering the active site or an additional C-terminal alpha helix in GsOYE. Not surprisingly, the active site of CtOYE and GsOYE structures revealed high affinity toward anions caught from the mother liquor and trapped in the anion hole where electron-withdrawing groups such as carbonyl group are engaged. Ligands (para-hydroxybenzaldehyde and 2-methyl-cyclopenten-1-one) added on purpose to study complexes of GsOYE were detected in the enzyme catalytic cavity, stacking on top of the FMN cofactor, and support the key role of conserved residues and FMN cofactor in the catalysis.

-	~~Authors~~: ~~Robescu, M.R~~., ~~Niero~~, M., Hall, M., ~~Bergantino~~, E., ~~Cendron, L~~.	+	Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.,Robescu MS, Niero M, Hall M, Cendron L, Bergantino E Appl Microbiol Biotechnol. 2020 Jan 13. pii: 10.1007/s00253-019-10287-2. doi:, 10.1007/s00253-019-10287-2. PMID:31930452<ref>PMID:31930452</ref>

-	~~Description: Crystal structure~~ of ~~ene~~-~~reductase GsOYE from Galdieria sulphuraria in complex with 4~~-~~Hydroxybenzaldehyde~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Niero, M~~]]	+	<div class="pdbe-citations 6s31" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bergantino, E]]		[[Category: Bergantino, E]]
-	[[Category: Hall, M]]
	[[Category: Cendron, L]]		[[Category: Cendron, L]]
-	[[Category: Robescu, M.R]]	+	[[Category: Hall, M]]
		+	[[Category: Niero, M]]
		+	[[Category: Robescu, M R]]
		+	[[Category: Biocatalysis]]
		+	[[Category: Ene-reductase]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Old Yellow Enzyme]]

OCA: Protected "6s31" [edit=sysop:move=sysop]

2019-07-03T04:30:22Z

Protected "6s31" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 3 July 2019

OCA: New page: '''Unreleased structure''' The entry 6s31 is ON HOLD Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L. Description: Crystal structure of ene-reductase GsOYE from...

2019-07-03T04:30:20Z

New page: '''Unreleased structure''' The entry 6s31 is ON HOLD Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L. Description: Crystal structure of ene-reductase GsOYE from...

New page

'''Unreleased structure'''

The entry 6s31 is ON HOLD

Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L.

Description: Crystal structure of ene-reductase GsOYE from Galdieria sulphuraria in complex with 4-Hydroxybenzaldehyde
[[Category: Unreleased Structures]]
[[Category: Niero, M]]
[[Category: Bergantino, E]]
[[Category: Hall, M]]
[[Category: Cendron, L]]
[[Category: Robescu, M.R]]