6s32 - Revision history

OCA at 12:34, 24 January 2024

2024-01-24T12:34:03Z

←Older revision		Revision as of 12:34, 24 January 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chroococcidiopsis_thermalis Chroococcidiopsis thermalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S32 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chroococcidiopsis_thermalis Chroococcidiopsis thermalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S32 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Chro_0590 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54299 Chroococcidiopsis thermalis])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [https://pdbe.org/6s32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [https://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [https://pdbe.org/6s32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [https://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K9TVC9_CHRTP K9TVC9_CHRTP]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6s32" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6s32" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[NADPH dehydrogenase\|NADPH dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 28:
	[[Category: Chroococcidiopsis thermalis]]		[[Category: Chroococcidiopsis thermalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bergantino, E]]	+	[[Category: Bergantino E]]
-	[[Category: Cendron, L]]	+	[[Category: Cendron L]]
-	[[Category: Hall, M]]	+	[[Category: Hall M]]
-	[[Category: Niero, M]]	+	[[Category: Niero M]]
-	[[Category: Robescu~~, M R]]~~	+	[[Category: Robescu MR]]
-	~~[[Category: Biocatalysis]]~~	+
-	~~[[Category: Ene-reductase]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Old Yellow Enzyme~~]]	+

OCA at 10:27, 4 August 2021

2021-08-04T10:27:43Z

←Older revision		Revision as of 10:27, 4 August 2021
Line 3:		Line 3:
	<StructureSection load='6s32' size='340' side='right'caption='[[6s32]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='6s32' size='340' side='right'caption='[[6s32]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chroococcidiopsis_thermalis Chroococcidiopsis thermalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6S32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chroococcidiopsis_thermalis Chroococcidiopsis thermalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S32 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=~~BAM~~:BENZAMIDINE'>~~BAM~~</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Chro_0590 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54299 Chroococcidiopsis thermalis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Chro_0590 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54299 Chroococcidiopsis thermalis])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [~~http~~://pdbe.org/6s32 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [https://pdbe.org/6s32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [https://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:32, 19 February 2020

2020-02-19T07:32:06Z

←Older revision		Revision as of 07:32, 19 February 2020
Line 3:		Line 3:
	<StructureSection load='6s32' size='340' side='right'caption='[[6s32]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='6s32' size='340' side='right'caption='[[6s32]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S32 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chroococcidiopsis_thermalis Chroococcidiopsis thermalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S32 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Chro_0590 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54299 Chroococcidiopsis thermalis])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [http://pdbe.org/6s32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [http://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [http://pdbe.org/6s32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [http://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chroococcidiopsis thermalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bergantino, E]]		[[Category: Bergantino, E]]

OCA at 15:37, 29 January 2020

2020-01-29T15:37:52Z

←Older revision		Revision as of 15:37, 29 January 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6s32 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of ene-reductase CtOYE from Chroococcidiopsis thermalis.==
		+	<StructureSection load='6s32' size='340' side='right'caption='[[6s32]], [[Resolution\|resolution]] 1.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6s32]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S32 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S32 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BAM:BENZAMIDINE'>BAM</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s32 OCA], [http://pdbe.org/6s32 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s32 RCSB], [http://www.ebi.ac.uk/pdbsum/6s32 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s32 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Looking for new ene-reductases with uncovered features beneficial for biotechnological applications, by mining genomes of photosynthetic extremophile organisms, we identified two new Old Yellow Enzyme homologues: CtOYE, deriving from the cyanobacterium Chroococcidiopsis thermalis, and GsOYE, from the alga Galdieria sulphuraria. Both enzymes were produced and purified with very good yields and displayed catalytic activity on a broad substrate spectrum by reducing alpha,beta-unsaturated ketones, aldehydes, maleimides and nitroalkenes with good to excellent stereoselectivity. Both enzymes prefer NADPH but demonstrate a good acceptance of NADH as cofactor. CtOYE and GsOYE represent robust biocatalysts showing high thermostability, a wide range of pH optimum and good co-solvent tolerance. High resolution X-ray crystal structures of both enzymes have been determined, revealing conserved features of the classical OYE subfamily as well as unique properties, such as a very long loop entering the active site or an additional C-terminal alpha helix in GsOYE. Not surprisingly, the active site of CtOYE and GsOYE structures revealed high affinity toward anions caught from the mother liquor and trapped in the anion hole where electron-withdrawing groups such as carbonyl group are engaged. Ligands (para-hydroxybenzaldehyde and 2-methyl-cyclopenten-1-one) added on purpose to study complexes of GsOYE were detected in the enzyme catalytic cavity, stacking on top of the FMN cofactor, and support the key role of conserved residues and FMN cofactor in the catalysis.

-	~~Authors~~: ~~Robescu, M.R~~., ~~Niero~~, M., Hall, M., ~~Bergantino~~, E., ~~Cendron, L~~.	+	Two new ene-reductases from photosynthetic extremophiles enlarge the panel of old yellow enzymes: CtOYE and GsOYE.,Robescu MS, Niero M, Hall M, Cendron L, Bergantino E Appl Microbiol Biotechnol. 2020 Jan 13. pii: 10.1007/s00253-019-10287-2. doi:, 10.1007/s00253-019-10287-2. PMID:31930452<ref>PMID:31930452</ref>

-	~~Description: Crystal structure~~ of ~~ene-reductase CtOYE from Chroococcidiopsis thermalis~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Niero, M~~]]	+	<div class="pdbe-citations 6s32" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bergantino, E]]		[[Category: Bergantino, E]]
-	[[Category: Hall, M]]
	[[Category: Cendron, L]]		[[Category: Cendron, L]]
-	[[Category: Robescu, M.R]]	+	[[Category: Hall, M]]
		+	[[Category: Niero, M]]
		+	[[Category: Robescu, M R]]
		+	[[Category: Biocatalysis]]
		+	[[Category: Ene-reductase]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Old Yellow Enzyme]]

OCA: Protected "6s32" [edit=sysop:move=sysop]

2019-07-03T04:30:31Z

Protected "6s32" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 3 July 2019

OCA: New page: '''Unreleased structure''' The entry 6s32 is ON HOLD until Paper Publication Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L. Description: Crystal structure of ...

2019-07-03T04:30:29Z

New page: '''Unreleased structure''' The entry 6s32 is ON HOLD until Paper Publication Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L. Description: Crystal structure of ...

New page

'''Unreleased structure'''

The entry 6s32 is ON HOLD until Paper Publication

Authors: Robescu, M.R., Niero, M., Hall, M., Bergantino, E., Cendron, L.

Description: Crystal structure of ene-reductase CtOYE from Chroococcidiopsis thermalis.
[[Category: Unreleased Structures]]
[[Category: Niero, M]]
[[Category: Bergantino, E]]
[[Category: Hall, M]]
[[Category: Cendron, L]]
[[Category: Robescu, M.R]]