6sos - Revision history

OCA at 12:44, 24 January 2024

2024-01-24T12:44:59Z

←Older revision		Revision as of 12:44, 24 January 2024
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-	====	+	==Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide==
-	<StructureSection load='6sos' size='340' side='right'caption='[[6sos]]' scene=''>	+	<StructureSection load='6sos' size='340' side='right'caption='[[6sos]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6sos]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SOS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sos OCA], [https://pdbe.org/6sos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sos RCSB], [https://www.ebi.ac.uk/pdbsum/6sos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sos ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BE2:2-AMINOBENZOIC+ACID'>BE2</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sos OCA], [https://pdbe.org/6sos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sos RCSB], [https://www.ebi.ac.uk/pdbsum/6sos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sos ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The affinity system based on the artificial peptide ligand Strep-tag(R) II and engineered tetrameric streptavidin, known as Strep-Tactin(R), offers attractive applications for the study of recombinant proteins, from detection and purification to functional immobilization. To further improve binding of the Strep-tag II to streptavidin we have subjected two protruding loops that shape its ligand pocket for the peptide - instead of D-biotin recognized by the natural protein - to iterative random mutagenesis. Sequence analyses of hits from functional screening assays revealed several unexpected structural motifs, such as a disulfide bridge at the base of one loop, replacement of the crucial residue Trp120 by Gly and a two-residue deletion in the second loop. The mutant m1-9 (dubbed Strep-Tactin XT) showed strongly enhanced affinity towards the Strep-tag II, which was further boosted in case of the bivalent Twin-Strep-tag(R). Four representative streptavidin mutants were crystallized in complex with the Strep-tag II peptide and their X-ray structures were solved at high resolutions. In addition, the crystal structure of the complex between Strep-Tactin XT and the Twin-Strep-tag was elucidated, indicating a bivalent mode of binding and explaining the experimentally observed avidity effect. Our study illustrates the structural plasticity of streptavidin as a scaffold for ligand binding and reveals interaction modes that would have been difficult to predict. As result, Strep-Tactin XT offers a convenient reagent for the kinetically stable immobilization of recombinant proteins fused with the Twin-Strep-tag. The possibility of reversibly dissociating such complexes simply with D-biotin as a competing ligand enables functional studies in protein science as well as cell biology.
		+
		+	The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.,Schmidt TGM, Eichinger A, Schneider M, Bonet L, Carl U, Karthaus D, Theobald I, Skerra A J Mol Biol. 2021 Apr 30;433(9):166893. doi: 10.1016/j.jmb.2021.166893. Epub 2021 , Feb 24. PMID:33639211<ref>PMID:33639211</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6sos" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Streptomyces avidinii]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Eichinger A]]
		+	[[Category: Skerra A]]

OCA at 06:52, 17 March 2021

2021-03-17T06:52:17Z

←Older revision		Revision as of 06:52, 17 March 2021
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	<StructureSection load='6sos' size='340' side='right'caption='[[6sos]]' scene=''>		<StructureSection load='6sos' size='340' side='right'caption='[[6sos]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sos OCA], [~~http~~://pdbe.org/6sos PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6sos RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6sos PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6sos ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sos OCA], [https://pdbe.org/6sos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sos RCSB], [https://www.ebi.ac.uk/pdbsum/6sos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sos ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 09:31, 9 September 2020

2020-09-09T09:31:33Z

←Older revision		Revision as of 09:31, 9 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6sos ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6sos' size='340' side='right'caption='[[6sos]]' scene=''>
-	~~Authors~~: ~~Skerra, A~~.~~, Eichinger, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Engineered streptavidin variant (ENAGY) in complex with the Twin~~-~~Strep-tag peptide~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sos OCA], [http://pdbe.org/6sos PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sos RCSB], [http://www.ebi.ac.uk/pdbsum/6sos PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sos ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Eichinger, A~~]]	+	__TOC__
-	[[Category: ~~Skerra, A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:57, 22 July 2020

2020-07-22T05:57:35Z

←Older revision		Revision as of 05:57, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6sos is ON HOLD	+	The entry 6sos is ON HOLD until Paper Publication

	Authors: Skerra, A., Eichinger, A.		Authors: Skerra, A., Eichinger, A.

OCA: Protected "6sos" [edit=sysop:move=sysop]

2019-10-02T07:30:22Z

Protected "6sos" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:30, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6sos is ON HOLD Authors: Skerra, A., Eichinger, A. Description: Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide ...

2019-10-02T07:30:20Z

New page: '''Unreleased structure''' The entry 6sos is ON HOLD Authors: Skerra, A., Eichinger, A. Description: Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide ...

New page

'''Unreleased structure'''

The entry 6sos is ON HOLD

Authors: Skerra, A., Eichinger, A.

Description: Engineered streptavidin variant (ENAGY) in complex with the Twin-Strep-tag peptide
[[Category: Unreleased Structures]]
[[Category: Eichinger, A]]
[[Category: Skerra, A]]