6syg - Revision history

OCA at 12:50, 24 January 2024

2024-01-24T12:50:25Z

←Older revision		Revision as of 12:50, 24 January 2024
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	<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6SYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SYG FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~KCNH2~~, ~~ERG, ERG1, HERG (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [~~http~~://pdbe.org/6syg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6syg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [https://pdbe.org/6syg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [https://www.ebi.ac.uk/pdbsum/6syg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>
	</table>		</table>
-	== Disease ==
-	[[http://www.uniprot.org/uniprot/KCNH2_HUMAN KCNH2_HUMAN]] Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:[http://omim.org/entry/613688 613688]]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2.<ref>PMID:16361248</ref> <ref>PMID:9600240</ref> <ref>PMID:7889573</ref> <ref>PMID:8914737</ref> <ref>PMID:8635257</ref> <ref>PMID:8877771</ref> <ref>PMID:9024139</ref> <ref>PMID:9693036</ref> <ref>PMID:9544837</ref> <ref>PMID:9452080</ref> <ref>PMID:10086971</ref> <ref>PMID:10220144</ref> <ref>PMID:10187793</ref> <ref>PMID:10517660</ref> <ref>PMID:10735633</ref> <ref>PMID:10973849</ref> <ref>PMID:10862094</ref> <ref>PMID:10753933</ref> <ref>PMID:12062363</ref> <ref>PMID:12354768</ref> <ref>PMID:12621127</ref> <ref>PMID:15051636</ref> <ref>PMID:15840476</ref> <ref>PMID:22314138</ref> Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:[http://omim.org/entry/609620 609620]]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.<ref>PMID:14676148</ref> <ref>PMID:15828882</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~KCNH2_HUMAN KCNH2_HUMAN]~~] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). ~~Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.~~	+	[https://www.uniprot.org/uniprot/KCNH2_RAT KCNH2_RAT] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).[UniProtKB:Q12809]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6syg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6syg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ben-Bassat, A]]	+	[[Category: Ben-Bassat A]]
-	[[Category: Giladi, M]]	+	[[Category: Giladi M]]
-	[[Category: Haitin, Y]]	+	[[Category: Haitin Y]]
-	~~[[Category: Cnbhd]]~~	+
-	~~[[Category: Herg]]~~	+
-	~~[[Category: Intracellular domain]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:17, 15 April 2020

2020-04-15T07:17:30Z

←Older revision		Revision as of 07:17, 15 April 2020
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	<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6SYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SYG FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNH2, ERG, ERG1, HERG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~10116 Buffalo rat~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNH2, ERG, ERG1, HERG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [http://pdbe.org/6syg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [http://www.ebi.ac.uk/pdbsum/6syg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [http://pdbe.org/6syg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [http://www.ebi.ac.uk/pdbsum/6syg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KCNH2_HUMAN KCNH2_HUMAN]] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.		[[http://www.uniprot.org/uniprot/KCNH2_HUMAN KCNH2_HUMAN]] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human KCNH2 channels (hKCNH2, ether-a-go-go [EAG]-related gene, hERG) are best known for their contribution to cardiac action potential repolarization and have key roles in various pathologies. Like other KCNH family members, hKCNH2 channels contain a unique intracellular complex, consisting of an N-terminal eag domain and a C-terminal cyclic nucleotide-binding homology domain (CNBHD), which is crucial for channel function. Previous studies demonstrated that the CNBHD is occupied by an intrinsic ligand motif, in a self-liganded conformation, providing a structural mechanism for the lack of KCNH channel regulation by cyclic nucleotides. While there have been significant advancements in the structural and functional characterization of the CNBHD of KCNH channels, a high-resolution structure of the hKCNH2 intracellular complex has been missing. Here, we report the 1.5 A resolution structure of the hKCNH2 channel CNBHD. The structure reveals the canonical fold shared by other KCNH family members, where the spatial organization of the intrinsic ligand is preserved within the beta-roll region. Moreover, measurements of small-angle x-ray scattering profile in solution, as well as comparison with a recent NMR analysis of hKCNH2, revealed high agreement with the crystallographic structure, indicating an overall low flexibility in solution. Importantly, we identified a novel salt-bridge (E807-R863) which was not previously resolved in the NMR and cryo-EM structures. Electrophysiological analysis of charge-reversal mutations revealed the bridge's crucial role in hKCNH2 function. Moreover, comparison with other KCNH members revealed the structural conservation of this salt-bridge, consistent with its functional significance. Together with the available structure of the mouse KCNH1 intracellular complex and previous electrophysiological and spectroscopic studies of KCNH family members, we propose that this salt-bridge serves as a strategically positioned linchpin to support both the spatial organization of the intrinsic ligand and the maintenance of the intracellular complex interface.
		+
		+	Structure of KCNH2 cyclic nucleotide-binding homology domain reveals a functionally vital salt-bridge.,Ben-Bassat A, Giladi M, Haitin Y J Gen Physiol. 2020 Apr 6;152(4). pii: 151568. doi: 10.1085/jgp.201912505. PMID:32191791<ref>PMID:32191791</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6syg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Buffalo rat~~]]	+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ben-Bassat, A]]		[[Category: Ben-Bassat, A]]

OCA at 10:28, 18 March 2020

2020-03-18T10:28:20Z

←Older revision		Revision as of 10:28, 18 March 2020
Line 3:		Line 3:
	<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SYG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SYG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [http://pdbe.org/6syg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [http://www.ebi.ac.uk/pdbsum/6syg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNH2, ERG, ERG1, HERG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [http://pdbe.org/6syg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [http://www.ebi.ac.uk/pdbsum/6syg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/KCNH2_HUMAN KCNH2_HUMAN]] Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:[http://omim.org/entry/613688 613688]]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2.<ref>PMID:16361248</ref> <ref>PMID:9600240</ref> <ref>PMID:7889573</ref> <ref>PMID:8914737</ref> <ref>PMID:8635257</ref> <ref>PMID:8877771</ref> <ref>PMID:9024139</ref> <ref>PMID:9693036</ref> <ref>PMID:9544837</ref> <ref>PMID:9452080</ref> <ref>PMID:10086971</ref> <ref>PMID:10220144</ref> <ref>PMID:10187793</ref> <ref>PMID:10517660</ref> <ref>PMID:10735633</ref> <ref>PMID:10973849</ref> <ref>PMID:10862094</ref> <ref>PMID:10753933</ref> <ref>PMID:12062363</ref> <ref>PMID:12354768</ref> <ref>PMID:12621127</ref> <ref>PMID:15051636</ref> <ref>PMID:15840476</ref> <ref>PMID:22314138</ref> Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:[http://omim.org/entry/609620 609620]]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.<ref>PMID:14676148</ref> <ref>PMID:15828882</ref>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~KCNH2_RAT KCNH2_RAT~~]] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr~~) (By similarity~~).~~[UniProtKB:Q12809]~~	+	[[http://www.uniprot.org/uniprot/KCNH2_HUMAN KCNH2_HUMAN]] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ben-Bassat, A]]		[[Category: Ben-Bassat, A]]

OCA at 06:50, 4 March 2020

2020-03-04T06:50:01Z

←Older revision		Revision as of 06:50, 4 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6syg ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the Cyclic Nucleotide-Binding Homology Domain of the human KCNH2 channel==
-		+	<StructureSection load='6syg' size='340' side='right'caption='[[6syg]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6syg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SYG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6syg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6syg OCA], [http://pdbe.org/6syg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6syg RCSB], [http://www.ebi.ac.uk/pdbsum/6syg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6syg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KCNH2_RAT KCNH2_RAT]] Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).[UniProtKB:Q12809]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ben-Bassat, A]]
		+	[[Category: Giladi, M]]
		+	[[Category: Haitin, Y]]
		+	[[Category: Cnbhd]]
		+	[[Category: Herg]]
		+	[[Category: Intracellular domain]]
		+	[[Category: Transport protein]]

OCA: Protected "6syg" [edit=sysop:move=sysop]

2019-10-10T03:42:51Z

Protected "6syg" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:42, 10 October 2019

OCA: New page: '''Unreleased structure''' The entry 6syg is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2019-10-10T03:42:50Z

New page: '''Unreleased structure''' The entry 6syg is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6syg is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]