6tig - Revision history

OCA at 13:02, 24 January 2024

2024-01-24T13:02:53Z

←Older revision		Revision as of 13:02, 24 January 2024
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	<StructureSection load='6tig' size='340' side='right'caption='[[6tig]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6tig' size='340' side='right'caption='[[6tig]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tig]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Burcj Burcj~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6TIG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tig]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cenocepacia_J2315 Burkholderia cenocepacia J2315]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCAM0185 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216591 BURCJ])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6tig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tig OCA], [~~http~~://pdbe.org/6tig PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tig RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tig PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tig ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tig OCA], [https://pdbe.org/6tig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tig RCSB], [https://www.ebi.ac.uk/pdbsum/6tig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tig ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B4EH86_BURCJ B4EH86_BURCJ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Burcj~~]]	+	[[Category: Burkholderia cenocepacia J2315]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bermeo, R]]	+	[[Category: Bermeo R]]
-	[[Category: Varrot, A]]	+	[[Category: Varrot A]]
-	~~[[Category: Globo h]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Tnf-like~~]]	+

OCA at 16:23, 29 January 2020

2020-01-29T16:23:07Z

←Older revision		Revision as of 16:23, 29 January 2020
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	<StructureSection load='6tig' size='340' side='right'caption='[[6tig]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6tig' size='340' side='right'caption='[[6tig]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tig]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TIG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tig]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Burcj Burcj]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TIG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCAM0185 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216591 BURCJ])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tig OCA], [http://pdbe.org/6tig PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tig RCSB], [http://www.ebi.ac.uk/pdbsum/6tig PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tig ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tig OCA], [http://pdbe.org/6tig PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tig RCSB], [http://www.ebi.ac.uk/pdbsum/6tig PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tig ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lectins mediate adhesion of pathogens to host tissues, filling in a key role in the first steps of infection. Belonging to the opportunistic pathogen Burkholderia cenocepacia, BC2L-C is a superlectin with dual carbohydrate specificity, believed to mediate cross-linking between bacteria and host cells. Its C-terminal domain binds to bacterial mannosides while its N-terminal domain (BCL2-CN) recognizes fucosylated human epitopes. BC2L-CN presents a tumor necrosis factor alpha (TNF-) fold previously unseen in lectins with a novel fucose binding mode. We report, here, the production of a novel recombinant form of BC2L-CN (rBC2L-CN2), which allowed better protein stability and unprecedented co-crystallization with oligosaccharides. Isothermal calorimetry measurements showed no detrimental effect on ligand binding and data were obtained on the binding of Globo H hexasaccharide and l-galactose. Crystal structures of rBC2L-CN2 were solved in complex with two blood group antigens: H-type 1 and H-type 3 (Globo H) by X-ray crystallography. They provide new structural information on the binding site, of importance for the structural-based design of glycodrugs as new antimicrobials with antiadhesive properties.
		+
		+	BC2L-C N-Terminal Lectin Domain Complexed with Histo Blood Group Oligosaccharides Provides New Structural Information.,Bermeo R, Bernardi A, Varrot A Molecules. 2020 Jan 7;25(2). pii: molecules25020248. doi:, 10.3390/molecules25020248. PMID:31936166<ref>PMID:31936166</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tig" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burcj]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bermeo, R]]		[[Category: Bermeo, R]]

OCA at 16:20, 22 January 2020

2020-01-22T16:20:27Z

←Older revision		Revision as of 16:20, 22 January 2020
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-	'''Unreleased structure'''

-	~~The entry 6tig is ON HOLD until Dec 20 2020~~	+	==Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H-type 3) antigen==
-		+	<StructureSection load='6tig' size='340' side='right'caption='[[6tig]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors: varrot, A., Bermeo, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6tig]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TIG FirstGlance]. <br>
-	~~Description:~~ Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H-type 3) antigen	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tig OCA], [http://pdbe.org/6tig PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tig RCSB], [http://www.ebi.ac.uk/pdbsum/6tig PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tig ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bermeo, R]]
	[[Category: Varrot, A]]		[[Category: Varrot, A]]
-	[[Category: ~~Bermeo, R~~]]	+	[[Category: Globo h]]
		+	[[Category: Lectin]]
		+	[[Category: Sugar binding protein]]
		+	[[Category: Tnf-like]]

OCA at 05:48, 18 December 2019

2019-12-18T05:48:49Z

←Older revision		Revision as of 05:48, 18 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tig is ON HOLD	+	The entry 6tig is ON HOLD until Dec 20 2020

	Authors: varrot, A., Bermeo, R.		Authors: varrot, A., Bermeo, R.

OCA: Protected "6tig" [edit=sysop:move=sysop]

2019-12-04T06:24:20Z

Protected "6tig" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:24, 4 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tig is ON HOLD Authors: varrot, A., Bermeo, R. Description: Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H...

2019-12-04T06:24:18Z

New page: '''Unreleased structure''' The entry 6tig is ON HOLD Authors: varrot, A., Bermeo, R. Description: Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H...

New page

'''Unreleased structure'''

The entry 6tig is ON HOLD

Authors: varrot, A., Bermeo, R.

Description: Structure of the N terminal domain of Bc2L-C lectin (1-131) in complex with Globo H (H-type 3) antigen
[[Category: Unreleased Structures]]
[[Category: Varrot, A]]
[[Category: Bermeo, R]]