6tlf - Revision history

OCA at 13:04, 24 January 2024

2024-01-24T13:04:47Z

←Older revision		Revision as of 13:04, 24 January 2024
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	<StructureSection load='6tlf' size='340' side='right'caption='[[6tlf]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6tlf' size='340' side='right'caption='[[6tlf]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tlf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TLF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TLF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SFN, HME1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlf OCA], [~~http~~://pdbe.org/6tlf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tlf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tlf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlf OCA], [https://pdbe.org/6tlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tlf RCSB], [https://www.ebi.ac.uk/pdbsum/6tlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.	+	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6tlf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6tlf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mangani, S]]	+	[[Category: Mangani S]]
-	[[Category: Pozzi, C]]	+	[[Category: Pozzi C]]
-	[[Category: Tassone, G]]	+	[[Category: Tassone G]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: H14-3-3sigma]]~~	+
-	~~[[Category: Human protein]]~~	+
-	~~[[Category: Imp]]~~	+
-	~~[[Category: Inosine monophosphate]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 07:08, 29 April 2020

2020-04-29T07:08:35Z

←Older revision		Revision as of 07:08, 29 April 2020
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	<StructureSection load='6tlf' size='340' side='right'caption='[[6tlf]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6tlf' size='340' side='right'caption='[[6tlf]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tlf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6TLF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tlf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TLF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6tlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlf OCA], [http://pdbe.org/6tlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tlf RCSB], [http://www.ebi.ac.uk/pdbsum/6tlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlf ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SFN, HME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlf OCA], [http://pdbe.org/6tlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tlf RCSB], [http://www.ebi.ac.uk/pdbsum/6tlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]

OCA at 09:54, 18 March 2020

2020-03-18T09:54:20Z

←Older revision		Revision as of 09:54, 18 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6tlf is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==human 14-3-3 sigma isoform in complex with IMP==
		+	<StructureSection load='6tlf' size='340' side='right'caption='[[6tlf]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6tlf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TLF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tlf OCA], [http://pdbe.org/6tlf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tlf RCSB], [http://www.ebi.ac.uk/pdbsum/6tlf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tlf ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 14-3-3/c-Abl protein-protein interaction (PPI) is related to carcinogenesis and in particular to pathogenesis of chronic myeloid leukaemia (CML). Previous studies have demonstrated that molecules able to disrupt this interaction improve the nuclear translocation of c-Abl, inducing apoptosis in leukaemia cells. Through an X-ray crystallography screening program, we have identified two phosphate-containing compounds, inosine monophosphate (IMP) and pyridoxal phosphate (PLP), as binders of human 14-3-3sigma, by targeting the protein amphipathic groove. Interestingly, they also act as weak inhibitors of the 14-3-3/c-Abl PPI, demonstrated by NMR, SPR and FP data. A 37-compound library of PLP and IMP analogues was investigated using a FP assay, leading to the identification of three further molecules acting as weak inhibitors of the 14-3-3/c-Abl complex formation. The antiproliferative activity of IMP, PLP and the three derivatives was tested against K-562 cells, showing that the parent compounds had the most pronounced effect on tumour cells. PLP and IMP were also effective in promoting the c-Abl nuclear translocation in c-Abl overexpressing cells. Further, these compounds demonstrated low cytotoxicity on human Hs27 fibroblasts. In conclusion, our data suggest that 14-3-3sigma targeting compounds represent promising hits for further development of drugs against c-Abl-dependent cancers.

-	~~Authors: Tassone~~, G., ~~Pozzi~~, C., Mangani, S.	+	Identification of phosphate-containing compounds as new inhibitors of 14-3-3/c-Abl protein-protein interaction.,Iralde-Lorente L, Tassone G, Clementi L, Franci L, Munier CC, Cau Y, Mori M, Chiariello M, Angelucci A, Perry MWD, Pozzi C, Mangani S, Botta M ACS Chem Biol. 2020 Mar 6. doi: 10.1021/acschembio.0c00039. PMID:32142251<ref>PMID:32142251</ref>

-	~~Description: human 14~~-3-~~3 sigma isoform in complex with IMP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Tassone, G~~]]	+	<div class="pdbe-citations 6tlf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mangani, S]]		[[Category: Mangani, S]]
	[[Category: Pozzi, C]]		[[Category: Pozzi, C]]
		+	[[Category: Tassone, G]]
		+	[[Category: Complex]]
		+	[[Category: H14-3-3sigma]]
		+	[[Category: Human protein]]
		+	[[Category: Imp]]
		+	[[Category: Inosine monophosphate]]
		+	[[Category: Signaling protein]]

OCA: Protected "6tlf" [edit=sysop:move=sysop]

2019-12-11T13:36:39Z

Protected "6tlf" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:36, 11 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tlf is ON HOLD until Paper Publication Authors: Tassone, G., Pozzi, C., Mangani, S. Description: human 14-3-3 sigma isoform in complex with IMP [[...

2019-12-11T13:36:38Z

New page: '''Unreleased structure''' The entry 6tlf is ON HOLD until Paper Publication Authors: Tassone, G., Pozzi, C., Mangani, S. Description: human 14-3-3 sigma isoform in complex with IMP [[...

New page

'''Unreleased structure'''

The entry 6tlf is ON HOLD until Paper Publication

Authors: Tassone, G., Pozzi, C., Mangani, S.

Description: human 14-3-3 sigma isoform in complex with IMP
[[Category: Unreleased Structures]]
[[Category: Tassone, G]]
[[Category: Mangani, S]]
[[Category: Pozzi, C]]