6u3u - Revision history

OCA at 07:43, 11 October 2023

2023-10-11T07:43:40Z

←Older revision		Revision as of 07:43, 11 October 2023
Line 1:		Line 1:

-	====	+	==Crystal Structure of Shiga Toxin 2K==
-	<StructureSection load='6u3u' size='340' side='right'caption='[[6u3u]]' scene=''>	+	<StructureSection load='6u3u' size='340' side='right'caption='[[6u3u]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u3u]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U3U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3u OCA], [https://pdbe.org/6u3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u3u RCSB], [https://www.ebi.ac.uk/pdbsum/6u3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3u ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.287Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3u OCA], [https://pdbe.org/6u3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u3u RCSB], [https://www.ebi.ac.uk/pdbsum/6u3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/L0I969_ECOLX L0I969_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Shiga toxin (Stx) is the major virulence factor of Shiga toxin-producing Escherichia coli (STEC). Stx evolves rapidly and, as such, new subtypes continue to emerge that challenge the efficacy of existing disease management and surveillance strategies. A new subtype, Stx2k, was recently identified in E. coli isolated from a wide range of sources including diarrheal patients, animals, and raw meats, and was poorly detected by existing immunoassays. In this study, the structure of Stx2kE167Q was determined at 2.29 A resolution and the conservation of structure with Stx2a was revealed. A novel polyclonal antibody capable of neutralizing Stx2k and an immunoassay, with a 10-fold increase in sensitivity compared to assays using extant antibodies, were developed. Stx2k is less toxic than Stx2a in Vero cell assays but is similar to Stx2a in receptor-binding preference, thermostability, and acid tolerance. Although Stx2k does not appear to be as potent as Stx2a to Vero cells, the wide distribution and blended virulence profiles of the Stx2k-producing strains suggest that horizontal gene transfer through Stx2k-converting phages could result in the emergence of new and highly virulent pathogens. This study provides useful information and tools for early detection and control of Stx2k-producing E. coli, which could reduce public risk of infection by less-known STECs.
		+
		+	Structural and Functional Characterization of Stx2k, a New Subtype of Shiga Toxin 2.,Hughes AC, Zhang Y, Bai X, Xiong Y, Wang Y, Yang X, Xu Q, He X Microorganisms. 2019 Dec 18;8(1). pii: microorganisms8010004. doi:, 10.3390/microorganisms8010004. PMID:31861375<ref>PMID:31861375</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u3u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Shiga toxin 3D structures\|Shiga toxin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: He XH]]
		+	[[Category: Zhang YZ]]

OCA at 08:56, 18 February 2021

2021-02-18T08:56:19Z

←Older revision		Revision as of 08:56, 18 February 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6u3u ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6u3u' size='340' side='right'caption='[[6u3u]]' scene=''>
-	~~Authors~~: ~~Zhang, Y~~.Z.~~, He, X~~.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of Shiga Toxin 2K~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u3u OCA], [https://pdbe.org/6u3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u3u RCSB], [https://www.ebi.ac.uk/pdbsum/6u3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u3u ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Zhang~~, Y.Z]]	+	__TOC__
-	[[Category: ~~He, X.H~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:09, 2 October 2019

2019-10-02T08:09:51Z

←Older revision		Revision as of 08:09, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u3u is ON HOLD	+	The entry 6u3u is ON HOLD until Paper Publication

	Authors: Zhang, Y.Z., He, X.H.		Authors: Zhang, Y.Z., He, X.H.
Line 7:		Line 7:
	Description: Crystal Structure of Shiga Toxin 2K		Description: Crystal Structure of Shiga Toxin 2K
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: He, X.H]]
	[[Category: Zhang, Y.Z]]		[[Category: Zhang, Y.Z]]
		+	[[Category: He, X.H]]

OCA: Protected "6u3u" [edit=sysop:move=sysop]

2019-08-28T14:07:21Z

Protected "6u3u" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:07, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u3u is ON HOLD Authors: Zhang, Y.Z., He, X.H. Description: Crystal Structure of Shiga Toxin 2K Category: Unreleased Structures [[Category: He,...

2019-08-28T14:07:19Z

New page: '''Unreleased structure''' The entry 6u3u is ON HOLD Authors: Zhang, Y.Z., He, X.H. Description: Crystal Structure of Shiga Toxin 2K Category: Unreleased Structures [[Category: He,...

New page

'''Unreleased structure'''

The entry 6u3u is ON HOLD

Authors: Zhang, Y.Z., He, X.H.

Description: Crystal Structure of Shiga Toxin 2K
[[Category: Unreleased Structures]]
[[Category: He, X.H]]
[[Category: Zhang, Y.Z]]