6u41 - Revision history

OCA at 07:43, 11 October 2023

2023-10-11T07:43:48Z

←Older revision		Revision as of 07:43, 11 October 2023
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	<StructureSection load='6u41' size='340' side='right'caption='[[6u41]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6u41' size='340' side='right'caption='[[6u41]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u41]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U41 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U41 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u41]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U41 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U41 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1uow\|1uow]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SYT1, SVP65, SYT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u41 OCA], [https://pdbe.org/6u41 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u41 RCSB], [https://www.ebi.ac.uk/pdbsum/6u41 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u41 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u41 OCA], [~~http~~://pdbe.org/6u41 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u41 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u41 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u41 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SYT1_HUMAN SYT1_HUMAN]] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.	+	[https://www.uniprot.org/uniprot/SYT1_HUMAN SYT1_HUMAN] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6u41" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6u41" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Synaptotagmin 3D structures\|Synaptotagmin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bradberry~~, M M~~]]	+	[[Category: Bradberry MM]]
-	[[Category: Chapman~~, E R~~]]	+	[[Category: Chapman ER]]
-	[[Category: Dominguez~~, M J~~]]	+	[[Category: Dominguez MJ]]
-	[[Category: Sutton~~, R B]]~~	+	[[Category: Sutton RB]]
-	~~[[Category: C2 domain]]~~	+
-	~~[[Category: C2b]]~~	+
-	~~[[Category: Exocytosis]]~~	+
-	~~[[Category: Greek key~~]]	+

OCA at 11:35, 22 July 2020

2020-07-22T11:35:48Z

←Older revision		Revision as of 11:35, 22 July 2020
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	==1.7 angstrom structure of a pathogenic human Syt 1 C2B (D304G)==		==1.7 angstrom structure of a pathogenic human Syt 1 C2B (D304G)==
-	<StructureSection load='6u41' size='340' side='right'caption='[[6u41]]' scene=''>	+	<StructureSection load='6u41' size='340' side='right'caption='[[6u41]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U41 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U41 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u41]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U41 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U41 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u41 OCA], [http://pdbe.org/6u41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u41 RCSB], [http://www.ebi.ac.uk/pdbsum/6u41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u41 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1uow\|1uow]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYT1, SVP65, SYT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u41 OCA], [http://pdbe.org/6u41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u41 RCSB], [http://www.ebi.ac.uk/pdbsum/6u41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u41 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SYT1_HUMAN SYT1_HUMAN]] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	At neuronal synapses, synaptotagmin-1 (syt1) acts as a Ca(2+) sensor that synchronizes neurotransmitter release with Ca(2+) influx during action potential firing. Heterozygous missense mutations in syt1 have recently been associated with a severe but heterogeneous developmental syndrome, termed syt1-associated neurodevelopmental disorder. Well-defined pathogenic mechanisms, and the basis for phenotypic heterogeneity in this disorder, remain unknown. Here, we report the clinical, physiological, and biophysical characterization of three syt1 mutations from human patients. Synaptic transmission was impaired in neurons expressing mutant variants, which demonstrated potent, graded dominant-negative effects. Biophysical interrogation of the mutant variants revealed novel mechanistic features concerning the cooperative action, and functional specialization, of the tandem Ca(2+)-sensing domains of syt1. These mechanistic studies led to the discovery that a clinically approved K(+) channel antagonist is able to rescue the dominant-negative heterozygous phenotype. Our results establish a molecular cause, basis for phenotypic heterogeneity, and potential treatment approach for syt1-associated neurodevelopmental disorder.
		+
		+	Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental Disorder.,Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, Sutton RB, Chapman ER Neuron. 2020 Apr 21. pii: S0896-6273(20)30272-5. doi:, 10.1016/j.neuron.2020.04.003. PMID:32362337<ref>PMID:32362337</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u41" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bradberry MM]]	+	[[Category: Bradberry, M M]]
-	[[Category: Chapman ER]]	+	[[Category: Chapman, E R]]
-	[[Category: Dominguez MJ]]	+	[[Category: Dominguez, M J]]
-	[[Category: Sutton RB]]	+	[[Category: Sutton, R B]]
		+	[[Category: C2 domain]]
		+	[[Category: C2b]]
		+	[[Category: Exocytosis]]
		+	[[Category: Greek key]]

OCA at 05:32, 13 May 2020

2020-05-13T05:32:54Z

←Older revision		Revision as of 05:32, 13 May 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6u41 ~~is ON HOLD until Paper Publication~~	+	==1.7 angstrom structure of a pathogenic human Syt 1 C2B (D304G)==
-		+	<StructureSection load='6u41' size='340' side='right'caption='[[6u41]]' scene=''>
-	~~Authors~~: ~~Dominguez~~, M.J., ~~Bradberry~~, M.M., ~~Chapman, E~~.R., ~~Sutton, R~~.B.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U41 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U41 FirstGlance]. <br>
-	~~Description: 1.7 angstrom structure of a pathogenic human Syt 1 C2B (D304G)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u41 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u41 OCA], [http://pdbe.org/6u41 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u41 RCSB], [http://www.ebi.ac.uk/pdbsum/6u41 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u41 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Bradberry~~, M.M~~]]	+	__TOC__
-	[[Category: ~~Sutton, R.B~~]]	+	</StructureSection>
-	[[Category: ~~Chapman, E.R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dominguez, M.J~~]]	+	[[Category: Bradberry MM]]
		+	[[Category: Chapman ER]]
		+	[[Category: Dominguez MJ]]
		+	[[Category: Sutton RB]]

OCA at 08:10, 2 October 2019

2019-10-02T08:10:17Z

←Older revision		Revision as of 08:10, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u41 is ON HOLD	+	The entry 6u41 is ON HOLD until Paper Publication

	Authors: Dominguez, M.J., Bradberry, M.M., Chapman, E.R., Sutton, R.B.		Authors: Dominguez, M.J., Bradberry, M.M., Chapman, E.R., Sutton, R.B.

OCA: Protected "6u41" [edit=sysop:move=sysop]

2019-08-28T14:07:57Z

Protected "6u41" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:07, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u41 is ON HOLD Authors: Dominguez, M.J., Bradberry, M.M., Chapman, E.R., Sutton, R.B. Description: 1.7 angstrom structure of a pathogenic human Sy...

2019-08-28T14:07:56Z

New page: '''Unreleased structure''' The entry 6u41 is ON HOLD Authors: Dominguez, M.J., Bradberry, M.M., Chapman, E.R., Sutton, R.B. Description: 1.7 angstrom structure of a pathogenic human Sy...

New page

'''Unreleased structure'''

The entry 6u41 is ON HOLD

Authors: Dominguez, M.J., Bradberry, M.M., Chapman, E.R., Sutton, R.B.

Description: 1.7 angstrom structure of a pathogenic human Syt 1 C2B (D304G)
[[Category: Unreleased Structures]]
[[Category: Bradberry, M.M]]
[[Category: Sutton, R.B]]
[[Category: Chapman, E.R]]
[[Category: Dominguez, M.J]]