6u9e - Revision history

OCA at 09:29, 20 March 2024

2024-03-20T09:29:01Z

←Older revision		Revision as of 09:29, 20 March 2024
Line 3:		Line 3:
	<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>		<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"francisella_tularensis_subsp~~._novicida~~"_(larson_et_al._1955)_sjostedt_2005 "francisella~~ tularensis subsp. novicida~~" (larson et al. 1955) sjostedt 2005~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Francisella_tularensis_subsp._novicida Francisella tularensis subsp. novicida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pdpA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~264~~ "~~Francisella tularensis subsp. novicida~~" ~~(Larson et al. 1955) Sjostedt 2005])~~, ~~B4919_03525 (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.21Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [https://pdbe.org/6u9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [https://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [https://pdbe.org/6u9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [https://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q7X3I9_FRANO Q7X3I9_FRANO]
-	Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella T6SS central spike complex, obtained by cryo-electron microscopy. Our structural and functional studies demonstrate that, unlike the single-protein spike composition of other T6SS subtypes, Francisella T6SS's central spike is formed by two proteins, PdpA and VgrG, akin to T4-bacteriophage gp27 and gp5, respectively, and that PdpA has unique characteristics, including a putative cargo within its cavity and an N-terminal helical lid. Structure-guided mutagenesis demonstrates that the PdpA N-terminal lid and C-terminal spike are essential to Francisella T6SS function. PdpA is thus both an adaptor, connecting VgrG to the tube, and a likely carrier of secreted cargo. These findings are important to understanding Francisella pathogenicity and designing therapeutics to combat tularemia.	+
-		+
-	~~Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo.,Yang X, Clemens DL, Lee BY, Cui Y, Zhou ZH, Horwitz MA Structure. 2019 Dec 3;27(12)~~:~~1811-1819.e6. doi: 10.1016~~/~~j.str.2019.10.007. Epub, 2019 Oct 31. PMID:31677891<ref>PMID:31677891<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6u9e" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Francisella tularensis subsp. novicida]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Clemens~~, D L~~]]	+	[[Category: Clemens DL]]
-	[[Category: Cui, Y]]	+	[[Category: Cui Y]]
-	[[Category: Horwitz~~, M A~~]]	+	[[Category: Horwitz MA]]
-	[[Category: Lee, B Y]]	+	[[Category: Lee B-Y]]
-	[[Category: Yang, X]]	+	[[Category: Yang X]]
-	[[Category: Zhou~~, Z H]]~~	+	[[Category: Zhou ZH]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: T6ss central spike]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:58, 5 May 2021

2021-05-05T08:58:12Z

←Older revision		Revision as of 08:58, 5 May 2021
Line 3:		Line 3:
	<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>		<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"francisella_tularensis_subsp._novicida"_(larson_et_al._1955)_sjostedt_2005 "francisella tularensis subsp. novicida" (larson et al. 1955) sjostedt 2005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [~~http~~://pdbe.org/6u9e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pdpA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005]), B4919_03525 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=264 "Francisella tularensis subsp. novicida" (Larson et al. 1955) Sjostedt 2005])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [https://pdbe.org/6u9e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [https://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Francisella bacteria rely on a phylogenetically distinct type VI secretion system (T6SS) to escape host phagosomes and cause the fatal disease tularemia, but the structural and molecular mechanisms involved are unknown. Here we report the atomic structure of the Francisella T6SS central spike complex, obtained by cryo-electron microscopy. Our structural and functional studies demonstrate that, unlike the single-protein spike composition of other T6SS subtypes, Francisella T6SS's central spike is formed by two proteins, PdpA and VgrG, akin to T4-bacteriophage gp27 and gp5, respectively, and that PdpA has unique characteristics, including a putative cargo within its cavity and an N-terminal helical lid. Structure-guided mutagenesis demonstrates that the PdpA N-terminal lid and C-terminal spike are essential to Francisella T6SS function. PdpA is thus both an adaptor, connecting VgrG to the tube, and a likely carrier of secreted cargo. These findings are important to understanding Francisella pathogenicity and designing therapeutics to combat tularemia.
		+
		+	Atomic Structure of the Francisella T6SS Central Spike Reveals a Unique alpha-Helical Lid and a Putative Cargo.,Yang X, Clemens DL, Lee BY, Cui Y, Zhou ZH, Horwitz MA Structure. 2019 Dec 3;27(12):1811-1819.e6. doi: 10.1016/j.str.2019.10.007. Epub, 2019 Oct 31. PMID:31677891<ref>PMID:31677891</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u9e" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>

OCA at 06:42, 11 April 2020

2020-04-11T06:42:51Z

←Older revision		Revision as of 06:42, 11 April 2020
Line 3:		Line 3:
	<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>		<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6U9E FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [http://pdbe.org/6u9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [http://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [http://pdbe.org/6u9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [http://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 01:08, 7 March 2020

2020-03-07T01:08:29Z

←Older revision		Revision as of 01:08, 7 March 2020
Line 1:		Line 1:

	==Structure of PdpA-VgrG Complex, Lidless==		==Structure of PdpA-VgrG Complex, Lidless==
-	<~~StructureSection~~ load='6u9e' size='340' side='right'caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>	+	<SX load='6u9e' size='340' side='right' viewer='molstar' caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U9E FirstGlance]. <br>		<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
Line 7:		Line 7:
	</table>		</table>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Clemens, D L]]		[[Category: Clemens, D L]]

OCA at 07:05, 23 October 2019

2019-10-23T07:05:27Z

←Older revision		Revision as of 07:05, 23 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6u9e is ~~ON HOLD until Paper Publication~~	+	==Structure of PdpA-VgrG Complex, Lidless==
-		+	<StructureSection load='6u9e' size='340' side='right'caption='[[6u9e]], [[Resolution\|resolution]] 4.21Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6u9e]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U9E FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u9e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9e OCA], [http://pdbe.org/6u9e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9e RCSB], [http://www.ebi.ac.uk/pdbsum/6u9e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9e ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Clemens, D L]]
		+	[[Category: Cui, Y]]
		+	[[Category: Horwitz, M A]]
		+	[[Category: Lee, B Y]]
		+	[[Category: Yang, X]]
		+	[[Category: Zhou, Z H]]
		+	[[Category: Complex]]
		+	[[Category: T6ss central spike]]
		+	[[Category: Transport protein]]

OCA: Protected "6u9e" [edit=sysop:move=sysop]

2019-10-02T08:25:36Z

Protected "6u9e" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:25, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6u9e is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2019-10-02T08:25:34Z

New page: '''Unreleased structure''' The entry 6u9e is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6u9e is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]