6ueb - Revision history

OCA at 09:29, 20 March 2024

2024-03-20T09:29:25Z

←Older revision		Revision as of 09:29, 20 March 2024
Line 3:		Line 3:
	<SX load='6ueb' size='340' side='right' viewer='molstar' caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6ueb' size='340' side='right' viewer='molstar' caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Rabv Rabv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rabies_virus_SAD_B19 Rabies virus SAD B19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UEB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~UNK~~:~~UNKNOWN~~'>~~UNK~~</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ueb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ueb OCA], [~~http~~://pdbe.org/6ueb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ueb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ueb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ueb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ueb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ueb OCA], [https://pdbe.org/6ueb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ueb RCSB], [https://www.ebi.ac.uk/pdbsum/6ueb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ueb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~L_RABVS L_RABVS~~]~~] RNA-directed RNA~~ polymerase that ~~catalyzes the~~ transcription ~~of viral mRNAs, their capping~~ and ~~polyadenylation~~. ~~The template is composed of~~ the ~~viral~~ RNA ~~tightly encapsidated by the nucleoprotein (N). The viral~~ polymerase ~~binds~~ to the ~~genomic~~ RNA ~~at the 3' leader promoter,~~ and ~~transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is~~ the ~~most transcribed~~, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA ~~chain length has reached few nucleotides~~. ~~Ribose 2'~~-~~O methylation of viral mRNA cap precedes~~ and ~~facilitates subsequent guanine~~-~~N-7 methylation, both acticities being carried~~ by the ~~viral polymerase. Polyadenylation of mRNAs occur~~ by ~~a stuttering mechanism at a slipery stop site present at~~ the ~~end viral genes. After finishing transcription~~ of ~~a mRNA,~~ the polymerase can resume transcription of the downstream gene.[UniProtKB:P03523] RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. ~~In this mode~~, ~~the polymerase replicates the whole viral genome without recognizing transcriptional signals~~, ~~and the replicated genome is not caped or polyadenylated.[UniProtKB:P03523]~~	+	[https://www.uniprot.org/uniprot/PHOSP_RABVS PHOSP_RABVS] Non catalytic polymerase cofactor and regulatory protein that plays a role in viral transcription and replication. Stabilizes the RNA polymerase L to the N-RNA template and binds the soluble protein N, preventing it from encapsidating non-genomic RNA. Also inhibits host IFN-alpha and IFN-beta signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Might be involved, through interaction with host dynein, in intracellular microtubule-dependent virus transport of incoming virus from the synapse toward the cell body (By similarity). Inhibits interferon induction pathways by interacting with host TBK1 and preventing the formation of dynamic cytoplasmic condensates that have liquid properties and that are essential for interferon production (PubMed:36640307).<ref>PMID:15557246</ref> <ref>PMID:36640307</ref>
-	~~<div style="background~~-~~color:#fffaf0;">~~	+
-	~~== Publication Abstract~~ from ~~PubMed ==~~	+
-	~~Nonsegmented negative-stranded (NNS) RNA viruses, among them~~ the ~~virus that causes rabies (RABV), include many deadly human pathogens. The large polymerase (L) proteins of NNS RNA viruses carry all of~~ the ~~enzymatic functions required for viral messenger RNA~~ (~~mRNA~~) ~~transcription and replication: RNA polymerization, mRNA capping, and cap methylation~~. ~~We describe here a complete structure of RABV L bound with its phosphoprotein cofactor (P), determined~~ by ~~electron cryo-microscopy at 3.3 A resolution. The complex closely resembles the vesicular stomatitis virus (VSV) L-P, the one other known full-length NNS-RNA L-protein structure,~~ with ~~key local differences (e.g., in L-P interactions). Like~~ the ~~VSV L-P structure, the RABV complex analyzed here represents a preinitiation conformation. Comparison with the likely elongation state, seen in two structures~~ of ~~pneumovirus L-P complexes, suggests differences between priming/initiation~~ and ~~elongation complexes. Analysis of internal cavities within RABV L suggests distinct template and product entry and exit pathways during transcription and replication.~~	+
-		+
-	~~Structure of a rabies virus polymerase complex from electron cryo-microscopy.,Horwitz JA, Jenni S, Harrison SC, Whelan SPJ Proc Natl Acad Sci U S A. 2020 Jan 28;117~~(4)~~:2099-2107~~. ~~doi:, 10.1073/pnas.1918809117. Epub 2020 Jan 17. PMID:31953264~~<ref>PMID:~~31953264~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6ueb" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 15:
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rabv~~]]	+	[[Category: Rabies virus SAD B19]]
-	[[Category: Harrison~~, S C~~]]	+	[[Category: Harrison SC]]
-	[[Category: Horwitz~~, J A]]~~	+	[[Category: Horwitz JA]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Polymerase]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 06:46, 11 April 2020

2020-04-11T06:46:57Z

←Older revision		Revision as of 06:46, 11 April 2020
Line 3:		Line 3:
	<SX load='6ueb' size='340' side='right' viewer='molstar' caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6ueb' size='340' side='right' viewer='molstar' caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabv Rabv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6UEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabv Rabv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UEB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ueb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ueb OCA], [http://pdbe.org/6ueb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ueb RCSB], [http://www.ebi.ac.uk/pdbsum/6ueb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ueb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ueb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ueb OCA], [http://pdbe.org/6ueb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ueb RCSB], [http://www.ebi.ac.uk/pdbsum/6ueb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ueb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:09, 7 March 2020

2020-03-07T01:09:54Z

←Older revision		Revision as of 01:09, 7 March 2020
Line 1:		Line 1:

	==Structure of Rabies SAD-B19 L-P complex from cryo-EM==		==Structure of Rabies SAD-B19 L-P complex from cryo-EM==
-	<~~StructureSection~~ load='6ueb' size='340' side='right'caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6ueb' size='340' side='right' viewer='molstar' caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabv Rabv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEB FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabv Rabv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEB FirstGlance]. <br>
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Rabv]]		[[Category: Rabv]]

OCA at 04:46, 13 February 2020

2020-02-13T04:46:30Z

←Older revision		Revision as of 04:46, 13 February 2020
Line 3:		Line 3:
	<StructureSection load='6ueb' size='340' side='right'caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6ueb' size='340' side='right'caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rabv Rabv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Rabv]]
	[[Category: Harrison, S C]]		[[Category: Harrison, S C]]
	[[Category: Horwitz, J A]]		[[Category: Horwitz, J A]]

OCA at 08:12, 5 February 2020

2020-02-05T08:12:23Z

←Older revision		Revision as of 08:12, 5 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ueb is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of Rabies SAD-B19 L-P complex from cryo-EM==
		+	<StructureSection load='6ueb' size='340' side='right'caption='[[6ueb]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ueb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UEB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ueb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ueb OCA], [http://pdbe.org/6ueb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ueb RCSB], [http://www.ebi.ac.uk/pdbsum/6ueb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ueb ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/L_RABVS L_RABVS]] RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.[UniProtKB:P03523] RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.[UniProtKB:P03523]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nonsegmented negative-stranded (NNS) RNA viruses, among them the virus that causes rabies (RABV), include many deadly human pathogens. The large polymerase (L) proteins of NNS RNA viruses carry all of the enzymatic functions required for viral messenger RNA (mRNA) transcription and replication: RNA polymerization, mRNA capping, and cap methylation. We describe here a complete structure of RABV L bound with its phosphoprotein cofactor (P), determined by electron cryo-microscopy at 3.3 A resolution. The complex closely resembles the vesicular stomatitis virus (VSV) L-P, the one other known full-length NNS-RNA L-protein structure, with key local differences (e.g., in L-P interactions). Like the VSV L-P structure, the RABV complex analyzed here represents a preinitiation conformation. Comparison with the likely elongation state, seen in two structures of pneumovirus L-P complexes, suggests differences between priming/initiation and elongation complexes. Analysis of internal cavities within RABV L suggests distinct template and product entry and exit pathways during transcription and replication.

-	~~Authors:~~ Horwitz, ~~J.A.~~, Harrison, S.C.	+	Structure of a rabies virus polymerase complex from electron cryo-microscopy.,Horwitz JA, Jenni S, Harrison SC, Whelan SPJ Proc Natl Acad Sci U S A. 2020 Jan 28;117(4):2099-2107. doi:, 10.1073/pnas.1918809117. Epub 2020 Jan 17. PMID:31953264<ref>PMID:31953264</ref>

-	~~Description: Structure~~ of ~~Rabies SAD-B19 L~~-~~P complex from cryo~~-EM	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Harrison, S.C]]	+	<div class="pdbe-citations 6ueb" style="background-color:#fffaf0;"></div>
-	[[Category: Horwitz, J.A]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Harrison, S C]]
		+	[[Category: Horwitz, J A]]
		+	[[Category: Complex]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Polymerase]]
		+	[[Category: Viral protein]]

OCA: Protected "6ueb" [edit=sysop:move=sysop]

2019-10-02T08:41:24Z

Protected "6ueb" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:41, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6ueb is ON HOLD until Paper Publication Authors: Horwitz, J.A., Harrison, S.C. Description: Structure of Rabies SAD-B19 L-P complex from cryo-EM [[...

2019-10-02T08:41:22Z

New page: '''Unreleased structure''' The entry 6ueb is ON HOLD until Paper Publication Authors: Horwitz, J.A., Harrison, S.C. Description: Structure of Rabies SAD-B19 L-P complex from cryo-EM [[...

New page

'''Unreleased structure'''

The entry 6ueb is ON HOLD until Paper Publication

Authors: Horwitz, J.A., Harrison, S.C.

Description: Structure of Rabies SAD-B19 L-P complex from cryo-EM
[[Category: Unreleased Structures]]
[[Category: Harrison, S.C]]
[[Category: Horwitz, J.A]]