OCA at 07:53, 11 October 2023

2023-10-11T07:53:13Z

←Older revision		Revision as of 07:53, 11 October 2023
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-	====	+	==Crystal structure of RHOG and ELMO complex==
-	<StructureSection load='6uka' size='340' side='right'caption='[[6uka]]' scene=''>	+	<StructureSection load='6uka' size='340' side='right'caption='[[6uka]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uka]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UKA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6uka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uka OCA], [~~http~~://pdbe.org/6uka PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uka RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uka PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uka ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uka OCA], [https://pdbe.org/6uka PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uka RCSB], [https://www.ebi.ac.uk/pdbsum/6uka PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uka ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RHOG_HUMAN RHOG_HUMAN] Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.<ref>PMID:15133129</ref> <ref>PMID:17074883</ref> <ref>PMID:17875742</ref> <ref>PMID:20679435</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DOCK (dedicator of cytokinesis) proteins are multidomain guanine nucleotide exchange factors (GEFs) for RHO GTPases that regulate intracellular actin dynamics. DOCK proteins share catalytic (DOCK(DHR2)) and membrane-associated (DOCK(DHR1)) domains. The structurally-related DOCK1 and DOCK2 GEFs are specific for RAC, and require ELMO (engulfment and cell motility) proteins for function. The N-terminal RAS-binding domain (RBD) of ELMO (ELMO(RBD)) interacts with RHOG to modulate DOCK1/2 activity. Here, we determine the cryo-EM structures of DOCK2-ELMO1 alone, and as a ternary complex with RAC1, together with the crystal structure of a RHOG-ELMO2(RBD) complex. The binary DOCK2-ELMO1 complex adopts a closed, auto-inhibited conformation. Relief of auto-inhibition to an active, open state, due to a conformational change of the ELMO1 subunit, exposes binding sites for RAC1 on DOCK2(DHR2), and RHOG and BAI GPCRs on ELMO1. Our structure explains how up-stream effectors, including DOCK2 and ELMO1 phosphorylation, destabilise the auto-inhibited state to promote an active GEF.
		+
		+	Structure of the DOCK2-ELMO1 complex provides insights into regulation of the auto-inhibited state.,Chang L, Yang J, Jo CH, Boland A, Zhang Z, McLaughlin SH, Abu-Thuraia A, Killoran RC, Smith MJ, Cote JF, Barford D Nat Commun. 2020 Jul 10;11(1):3464. doi: 10.1038/s41467-020-17271-9. PMID:32651375<ref>PMID:32651375</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uka" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
		+	*[[Rho GTPase 3D structures\|Rho GTPase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Jo CH]]
		+	[[Category: Killoran RC]]
		+	[[Category: Smith MJ]]

OCA at 09:47, 12 August 2020

2020-08-12T09:47:05Z

←Older revision		Revision as of 09:47, 12 August 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6uka ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6uka' size='340' side='right'caption='[[6uka]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uka FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uka OCA], [http://pdbe.org/6uka PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uka RCSB], [http://www.ebi.ac.uk/pdbsum/6uka PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uka ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:16, 6 November 2019

2019-11-06T05:16:53Z

←Older revision		Revision as of 05:16, 6 November 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uka is ON HOLD	+	The entry 6uka is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6uka" [edit=sysop:move=sysop]

2019-10-16T04:35:19Z

Protected "6uka" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6uka is ON HOLD Authors: Description: Category: Unreleased Structures

2019-10-16T04:35:17Z

New page: '''Unreleased structure''' The entry 6uka is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6uka is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6uka - Revision history

OCA at 07:53, 11 October 2023

OCA at 09:47, 12 August 2020

OCA at 05:16, 6 November 2019

OCA: Protected "6uka" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6uka is ON HOLD Authors: Description: Category: Unreleased Structures