6uko - Revision history

OCA at 14:57, 13 March 2024

2024-03-13T14:57:33Z

←Older revision		Revision as of 14:57, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6uko' size='340' side='right'caption='[[6uko]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='6uko' size='340' side='right'caption='[[6uko]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uko]] is a 7 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6UKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uko]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UKO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ADP:ADENOSINE~~-~~5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.4Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Bcs1l ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id~~=~~10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6uko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uko OCA], [~~http~~://pdbe.org/6uko PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uko RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uko PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uko ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uko OCA], [https://pdbe.org/6uko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uko RCSB], [https://www.ebi.ac.uk/pdbsum/6uko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BCS1_MOUSE BCS1_MOUSE]] Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex (By similarity).	+	[https://www.uniprot.org/uniprot/BCS1_MOUSE BCS1_MOUSE] Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron-sulfur protein (ISP), a subunit of the respiratory Complex III. How Bcs1 translocates ISP across the membrane is unknown. Here we report structures of mouse Bcs1 in two different conformations, representing three nucleotide states. The apo and ADP-bound structures reveal a homo-heptamer and show a large putative substrate-binding cavity accessible to the matrix space. ATP binding drives a contraction of the cavity by concerted motion of the ATPase domains, which could push substrate across the membrane. Our findings shed light on the potential mechanism of translocating folded proteins across a membrane, offer insights into the assembly process of Complex III and allow mapping of human disease-associated mutations onto the Bcs1 structure.	+
-		+
-	Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein.,Tang WK, Borgnia MJ, Hsu AL, Esser L, Fox T, de Val N, Xia D Nat Struct Mol Biol. 2020 Feb;27(2):202-209. doi: 10.1038/s41594-020-0373-0. Epub, 2020 Feb 10. PMID:32042153<ref>PMID:32042153</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6uko" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Esser, L]]	+	[[Category: Esser L]]
-	[[Category: Xia, D]]	+	[[Category: Xia D]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Cytochrome bc1]]~~	+
-	~~[[Category: Rieske~~]]	+

OCA at 10:33, 26 February 2020

2020-02-26T10:33:45Z

←Older revision		Revision as of 10:33, 26 February 2020
Line 3:		Line 3:
	<StructureSection load='6uko' size='340' side='right'caption='[[6uko]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='6uko' size='340' side='right'caption='[[6uko]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uko]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uko]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UKO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bcs1l ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uko OCA], [http://pdbe.org/6uko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uko RCSB], [http://www.ebi.ac.uk/pdbsum/6uko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uko ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uko OCA], [http://pdbe.org/6uko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uko RCSB], [http://www.ebi.ac.uk/pdbsum/6uko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BCS1_MOUSE BCS1_MOUSE]] Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex (By similarity).		[[http://www.uniprot.org/uniprot/BCS1_MOUSE BCS1_MOUSE]] Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron-sulfur protein (ISP), a subunit of the respiratory Complex III. How Bcs1 translocates ISP across the membrane is unknown. Here we report structures of mouse Bcs1 in two different conformations, representing three nucleotide states. The apo and ADP-bound structures reveal a homo-heptamer and show a large putative substrate-binding cavity accessible to the matrix space. ATP binding drives a contraction of the cavity by concerted motion of the ATPase domains, which could push substrate across the membrane. Our findings shed light on the potential mechanism of translocating folded proteins across a membrane, offer insights into the assembly process of Complex III and allow mapping of human disease-associated mutations onto the Bcs1 structure.
		+
		+	Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein.,Tang WK, Borgnia MJ, Hsu AL, Esser L, Fox T, de Val N, Xia D Nat Struct Mol Biol. 2020 Feb;27(2):202-209. doi: 10.1038/s41594-020-0373-0. Epub, 2020 Feb 10. PMID:32042153<ref>PMID:32042153</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uko" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Esser, L]]		[[Category: Esser, L]]
	[[Category: Xia, D]]		[[Category: Xia, D]]

OCA at 04:28, 13 February 2020

2020-02-13T04:28:36Z

←Older revision		Revision as of 04:28, 13 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6uko is ~~ON HOLD until Paper Publication~~	+	==Structure analysis of full-length mouse bcs1 complex==
-		+	<StructureSection load='6uko' size='340' side='right'caption='[[6uko]], [[Resolution\|resolution]] 4.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6uko]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UKO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uko OCA], [http://pdbe.org/6uko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uko RCSB], [http://www.ebi.ac.uk/pdbsum/6uko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uko ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BCS1_MOUSE BCS1_MOUSE]] Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex (By similarity).
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Esser, L]]
		+	[[Category: Xia, D]]
		+	[[Category: Chaperone]]
		+	[[Category: Cytochrome bc1]]
		+	[[Category: Rieske]]

OCA at 05:10, 23 October 2019

2019-10-23T05:10:04Z

←Older revision		Revision as of 05:10, 23 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uko is ON HOLD	+	The entry 6uko is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6uko" [edit=sysop:move=sysop]

2019-10-16T04:36:15Z

Protected "6uko" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6uko is ON HOLD Authors: Description: Category: Unreleased Structures

2019-10-16T04:36:13Z

New page: '''Unreleased structure''' The entry 6uko is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6uko is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]