6vtp - Revision history

OCA at 08:19, 11 October 2023

2023-10-11T08:19:42Z

←Older revision		Revision as of 08:19, 11 October 2023
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	<StructureSection load='6vtp' size='340' side='right'caption='[[6vtp]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6vtp' size='340' side='right'caption='[[6vtp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6vtp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vtp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VTP FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LGALS7, PIG1, LGALS7B ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vtp OCA], [https://pdbe.org/6vtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vtp RCSB], [https://www.ebi.ac.uk/pdbsum/6vtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vtp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vtp OCA], [https://pdbe.org/6vtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vtp RCSB], [https://www.ebi.ac.uk/pdbsum/6vtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vtp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LEG7_HUMAN LEG7_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6vtp" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6vtp" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Calmettes, C]]	+	[[Category: Calmettes C]]
-	[[Category: Doucet, N]]	+	[[Category: Doucet N]]
-	[[Category: Pham~~, N T.H]]~~	+	[[Category: Pham NTH]]
-	~~[[Category: Disulfide bond]]~~	+
-	~~[[Category: G16c mutant]]~~	+
-	~~[[Category: Human galectin-7]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 14:07, 17 November 2021

2021-11-17T14:07:47Z

←Older revision		Revision as of 14:07, 17 November 2021
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	==Crystal structure of G16C human Galectin-7 mutant==		==Crystal structure of G16C human Galectin-7 mutant==
-	<StructureSection load='6vtp' size='340' side='right'caption='[[6vtp]]' scene=''>	+	<StructureSection load='6vtp' size='340' side='right'caption='[[6vtp]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VTP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vtp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VTP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vtp OCA], [https://pdbe.org/6vtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vtp RCSB], [https://www.ebi.ac.uk/pdbsum/6vtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vtp ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS7, PIG1, LGALS7B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vtp OCA], [https://pdbe.org/6vtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vtp RCSB], [https://www.ebi.ac.uk/pdbsum/6vtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vtp ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The design of allosteric modulators to control protein function is a key objective in drug discovery programs. Altering functionally essential allosteric residue networks provides unique protein family subtype specificity, minimizes unwanted off-target effects, and helps avert resistance acquisition typically plaguing drugs that target orthosteric sites. In this work, we used protein engineering and dimer interface mutations to positively and negatively modulate the immunosuppressive activity of the proapoptotic human galectin-7 (GAL-7). Using the PoPMuSiC and BeAtMuSiC algorithms, mutational sites and residue identity were computationally probed and predicted to either alter or stabilize the GAL-7 dimer interface. By designing a covalent disulfide bridge between protomers to control homodimer strength and stability, we demonstrate the importance of dimer interface perturbations on the allosteric network bridging the two opposite glycan-binding sites on GAL-7, resulting in control of induced apoptosis in Jurkat T cells. Molecular investigation of G16X GAL-7 variants using X-ray crystallography, biophysical, and computational characterization illuminates residues involved in dimer stability and allosteric communication, along with discrete long-range dynamic behaviors involving loops 1, 3, and 5. We show that perturbing the protein-protein interface between GAL-7 protomers can modulate its biological function, even when the overall structure and ligand-binding affinity remains unaltered. This study highlights new avenues for the design of galectin-specific modulators influencing both glycan-dependent and glycan-independent interactions.
		+
		+	Perturbing dimer interactions and allosteric communication modulates the immunosuppressive activity of human galectin-7.,Pham NTH, Letourneau M, Fortier M, Begin G, Al-Abdul-Wahid MS, Pucci F, Folch B, Rooman M, Chatenet D, St-Pierre Y, Lague P, Calmettes C, Doucet N J Biol Chem. 2021 Oct 19;297(5):101308. doi: 10.1016/j.jbc.2021.101308. PMID:34673030<ref>PMID:34673030</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6vtp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Calmettes C]]	+	[[Category: Calmettes, C]]
-	[[Category: Doucet N]]	+	[[Category: Doucet, N]]
-	[[Category: Pham ~~NTH~~]]	+	[[Category: Pham, N T.H]]
		+	[[Category: Disulfide bond]]
		+	[[Category: G16c mutant]]
		+	[[Category: Human galectin-7]]
		+	[[Category: Sugar binding protein]]

OCA at 06:16, 25 August 2021

2021-08-25T06:16:02Z

←Older revision		Revision as of 06:16, 25 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6vtp ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of G16C human Galectin-7 mutant==
-		+	<StructureSection load='6vtp' size='340' side='right'caption='[[6vtp]]' scene=''>
-	~~Authors~~: ~~Pham~~, N.T.H., ~~Calmettes~~, C., ~~Doucet~~, N.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VTP FirstGlance]. <br>
-	~~Description: Crystal structure of G16C human Galectin-7 mutant~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vtp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vtp OCA], [https://pdbe.org/6vtp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vtp RCSB], [https://www.ebi.ac.uk/pdbsum/6vtp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vtp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Doucet, N~~]]	+	__TOC__
-	[[Category: ~~Pham,~~ N~~.T.H~~]]	+	</StructureSection>
-	[[Category: ~~Calmettes, C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Calmettes C]]
		+	[[Category: Doucet N]]
		+	[[Category: Pham NTH]]

OCA at 06:03, 11 March 2020

2020-03-11T06:03:54Z

←Older revision		Revision as of 06:03, 11 March 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6vtp is ON HOLD	+	The entry 6vtp is ON HOLD until Paper Publication

	Authors: Pham, N.T.H., Calmettes, C., Doucet, N.		Authors: Pham, N.T.H., Calmettes, C., Doucet, N.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Doucet, N]]		[[Category: Doucet, N]]
-	[[Category: Calmettes, C]]
	[[Category: Pham, N.T.H]]		[[Category: Pham, N.T.H]]
		+	[[Category: Calmettes, C]]

OCA: Protected "6vtp" [edit=sysop:move=sysop]

2020-02-19T05:23:12Z

Protected "6vtp" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:23, 19 February 2020

OCA: New page: '''Unreleased structure''' The entry 6vtp is ON HOLD Authors: Pham, N.T.H., Calmettes, C., Doucet, N. Description: Crystal structure of G16C human Galectin-7 mutant [[Category: Unrelea...

2020-02-19T05:23:11Z

New page: '''Unreleased structure''' The entry 6vtp is ON HOLD Authors: Pham, N.T.H., Calmettes, C., Doucet, N. Description: Crystal structure of G16C human Galectin-7 mutant [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 6vtp is ON HOLD

Authors: Pham, N.T.H., Calmettes, C., Doucet, N.

Description: Crystal structure of G16C human Galectin-7 mutant
[[Category: Unreleased Structures]]
[[Category: Doucet, N]]
[[Category: Calmettes, C]]
[[Category: Pham, N.T.H]]