6vy0 - Revision history

OCA at 14:40, 6 March 2024

2024-03-06T14:40:38Z

←Older revision		Revision as of 14:40, 6 March 2024
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	<StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]], [[Resolution\|resolution]] 6.68Å' scene=''>		<StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]], [[Resolution\|resolution]] 6.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6vy0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Spitz Spitz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vy0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spirochaeta_thermophila_DSM_6578 Spirochaeta thermophila DSM 6578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[6vxz\|6vxz]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 6.68Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Spith_0644 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=869211 SPITZ])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [https://pdbe.org/6vy0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [https://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [~~http~~://pdbe.org/6vy0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/G0GA88_SPITZ G0GA88_SPITZ]
-	SthK, a cyclic nucleotide-modulated ion channel from Spirochaeta thermophila, activates slowly upon cAMP increase. This is reminiscent of the slow, cAMP-induced activation reported for the hyperpolarization-activated and cyclic nucleotide-gated channel HCN2 in the family of so-called pacemaker channels. Here, we investigate slow cAMP-induced activation in purified SthK channels using stopped-flow assays, mutagenesis, enzymatic catalysis and inhibition assays revealing that the cis/~~trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK~~. ~~We propose that SthK exists in two forms: trans Pro300 SthK with high ligand binding affinity and fast activation, and cis Pro300 SthK with low affinity and slow activation~~. ~~Following channel activation, the cis~~/trans equilibrium, catalyzed by prolyl isomerases, is shifted towards trans, while steady-state channel activity is unaffected. Our results reveal prolyl isomerization as a regulatory mechanism for SthK, and potentially eukaryotic HCN channels. This mechanism could contribute to electrical rhythmicity in cells.	+

-	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel.,Schmidpeter PAM, Rheinberger J, Nimigean CM Nat Commun. 2020 Dec 16;11(1):6401. doi: 10.1038/s41467-020-20104-4. PMID:33328472<ref>PMID:33328472</ref>	+	==See Also==
-		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6vy0" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Spitz~~]]	+	[[Category: Spirochaeta thermophila DSM 6578]]
-	[[Category: Nimigean~~, C M~~]]	+	[[Category: Nimigean CM]]
-	[[Category: Rheinberger, J]]	+	[[Category: Rheinberger J]]
-	[[Category: Schmidpeter~~, P A.M]]~~	+	[[Category: Schmidpeter PAM]]
-	~~[[Category: Transport protein~~]]	+

OCA at 06:01, 20 January 2021

2021-01-20T06:01:00Z

←Older revision		Revision as of 06:01, 20 January 2021
Line 1:		Line 1:

	==SthK P300A cyclic nucleotide-gated potassium channel in a putative active state, in complex with cAMP==		==SthK P300A cyclic nucleotide-gated potassium channel in a putative active state, in complex with cAMP==
-	<StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]]' scene=''>	+	<StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]], [[Resolution\|resolution]] 6.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vy0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Spitz Spitz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [http://pdbe.org/6vy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vxz\|6vxz]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Spith_0644 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=869211 SPITZ])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [http://pdbe.org/6vy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	SthK, a cyclic nucleotide-modulated ion channel from Spirochaeta thermophila, activates slowly upon cAMP increase. This is reminiscent of the slow, cAMP-induced activation reported for the hyperpolarization-activated and cyclic nucleotide-gated channel HCN2 in the family of so-called pacemaker channels. Here, we investigate slow cAMP-induced activation in purified SthK channels using stopped-flow assays, mutagenesis, enzymatic catalysis and inhibition assays revealing that the cis/trans conformation of a conserved proline in the cyclic nucleotide-binding domain determines the activation kinetics of SthK. We propose that SthK exists in two forms: trans Pro300 SthK with high ligand binding affinity and fast activation, and cis Pro300 SthK with low affinity and slow activation. Following channel activation, the cis/trans equilibrium, catalyzed by prolyl isomerases, is shifted towards trans, while steady-state channel activity is unaffected. Our results reveal prolyl isomerization as a regulatory mechanism for SthK, and potentially eukaryotic HCN channels. This mechanism could contribute to electrical rhythmicity in cells.
		+
		+	Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel.,Schmidpeter PAM, Rheinberger J, Nimigean CM Nat Commun. 2020 Dec 16;11(1):6401. doi: 10.1038/s41467-020-20104-4. PMID:33328472<ref>PMID:33328472</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6vy0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nimigean CM]]	+	[[Category: Spitz]]
-	[[Category: Rheinberger J]]	+	[[Category: Nimigean, C M]]
-	[[Category: Schmidpeter ~~PAM~~]]	+	[[Category: Rheinberger, J]]
		+	[[Category: Schmidpeter, P A.M]]
		+	[[Category: Transport protein]]

OCA at 07:59, 11 November 2020

2020-11-11T07:59:20Z

←Older revision		Revision as of 07:59, 11 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6vy0 is ~~ON HOLD until Paper Publication~~	+	==SthK P300A cyclic nucleotide-gated potassium channel in a putative active state, in complex with cAMP==
-		+	<StructureSection load='6vy0' size='340' side='right'caption='[[6vy0]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VY0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vy0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy0 OCA], [http://pdbe.org/6vy0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vy0 RCSB], [http://www.ebi.ac.uk/pdbsum/6vy0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nimigean CM]]
		+	[[Category: Rheinberger J]]
		+	[[Category: Schmidpeter PAM]]

OCA at 07:24, 1 April 2020

2020-04-01T07:24:28Z

←Older revision		Revision as of 07:24, 1 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6vy0 is ON HOLD	+	The entry 6vy0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6vy0" [edit=sysop:move=sysop]

2020-03-04T05:39:03Z

Protected "6vy0" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 4 March 2020

OCA: New page: '''Unreleased structure''' The entry 6vy0 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-03-04T05:39:02Z

New page: '''Unreleased structure''' The entry 6vy0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6vy0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]