6w3o - Revision history

OCA at 14:16, 18 October 2023

2023-10-18T14:16:12Z

←Older revision		Revision as of 14:16, 18 October 2023
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	<StructureSection load='6w3o' size='340' side='right'caption='[[6w3o]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='6w3o' size='340' side='right'caption='[[6w3o]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6w3o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W3O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6W3O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w3o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W3O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SKG:4-methylisoleucine'>SKG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.42Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D8X59_02240 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SKG:4-methylisoleucine'>SKG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w3o OCA], [~~http~~://pdbe.org/6w3o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6w3o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6w3o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6w3o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w3o OCA], [https://pdbe.org/6w3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w3o RCSB], [https://www.ebi.ac.uk/pdbsum/6w3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w3o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0P864_CAMJE Q0P864_CAMJE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6w3o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6w3o" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chemotaxis protein 3D structures\|Chemotaxis protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Khan~~, M F~~]]	+	[[Category: Khan MF]]
-	[[Category: Machuca~~, M A~~]]	+	[[Category: Machuca MA]]
-	[[Category: Rahman~~, M M~~]]	+	[[Category: Rahman MM]]
-	[[Category: Roujeinikova, A]]	+	[[Category: Roujeinikova A]]
-	~~[[Category: Bacterial chemotaxis]]~~	+
-	~~[[Category: Chemoreceptor]]~~	+
-	~~[[Category: Double cache]]~~	+
-	~~[[Category: Ligand binding domain]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 07:39, 27 May 2020

2020-05-27T07:39:58Z

←Older revision		Revision as of 07:39, 27 May 2020
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	==Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 4-methylisoleucine==		==Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 4-methylisoleucine==
-	<StructureSection load='6w3o' size='340' side='right'caption='[[6w3o]]' scene=''>	+	<StructureSection load='6w3o' size='340' side='right'caption='[[6w3o]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W3O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W3O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w3o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W3O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W3O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w3o OCA], [http://pdbe.org/6w3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w3o RCSB], [http://www.ebi.ac.uk/pdbsum/6w3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w3o ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SKG:4-methylisoleucine'>SKG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D8X59_02240 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w3o OCA], [http://pdbe.org/6w3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w3o RCSB], [http://www.ebi.ac.uk/pdbsum/6w3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w3o ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chemotaxis is an important virulence factor of the foodborne pathogen Campylobacter jejuni. Inactivation of chemoreceptor Tlp3 reduces the ability of C. jejuni to invade human and chicken cells and to colonise the jejunal mucosa of mice. Knowledge of the structure of the ligand-binding domain (LBD) of Tlp3 in complex with its ligands is essential for a full understanding of the molecular recognition underpinning chemotaxis. To date, the only structure in complex with a signal molecule is Tlp3 LBD bound to isoleucine. Here, we used in vitro and in silico screening to identify eight additional small molecules that signal through Tlp3 as attractants by directly binding to its LBD, and determined the crystal structures of their complexes. All new ligands (leucine, valine, alpha-amino-N-valeric acid, 4-methylisoleucine, beta-methylnorleucine, 3-methylisoleucine, alanine, and phenylalanine) are nonpolar amino acids chemically and structurally similar to isoleucine. X-ray crystallographic analysis revealed the hydrophobic side-chain binding pocket and conserved protein residues that interact with the ammonium and carboxylate groups of the ligands determine the specificity of this chemoreceptor. The uptake of hydrophobic amino acids plays an important role in intestinal colonisation by C. jejuni, and our study suggests that C. jejuni seeks out hydrophobic amino acids using chemotaxis.
		+
		+	Structure-Activity Relationship Study Reveals the Molecular Basis for Specific Sensing of Hydrophobic Amino Acids by the Campylobacter jejuni Chemoreceptor Tlp3.,Khan MF, Machuca MA, Rahman MM, Koc C, Norton RS, Smith BJ, Roujeinikova A Biomolecules. 2020 May 11;10(5). pii: biom10050744. doi: 10.3390/biom10050744. PMID:32403336<ref>PMID:32403336</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6w3o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Khan MF]]	+	[[Category: Khan, M F]]
-	[[Category: Machuca MA]]	+	[[Category: Machuca, M A]]
-	[[Category: Rahman MM]]	+	[[Category: Rahman, M M]]
-	[[Category: Roujeinikova A]]	+	[[Category: Roujeinikova, A]]
		+	[[Category: Bacterial chemotaxis]]
		+	[[Category: Chemoreceptor]]
		+	[[Category: Double cache]]
		+	[[Category: Ligand binding domain]]
		+	[[Category: Signaling protein]]

OCA at 05:50, 20 May 2020

2020-05-20T05:50:18Z

←Older revision		Revision as of 05:50, 20 May 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6w3o is ON HOLD until Paper Publication~~	+	==Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 4-methylisoleucine==
-		+	<StructureSection load='6w3o' size='340' side='right'caption='[[6w3o]]' scene=''>
-	~~Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W3O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W3O FirstGlance]. <br>
-	~~Description:~~ Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 4-methylisoleucine	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w3o OCA], [http://pdbe.org/6w3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w3o RCSB], [http://www.ebi.ac.uk/pdbsum/6w3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w3o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Khan~~, M.F~~]]	+	__TOC__
-	[[Category: ~~Roujeinikova, A~~]]	+	</StructureSection>
-	[[Category: Rahman~~, M.M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Machuca, M.~~A]]	+	[[Category: Khan MF]]
		+	[[Category: Machuca MA]]
		+	[[Category: Rahman MM]]
		+	[[Category: Roujeinikova A]]

OCA at 05:32, 8 April 2020

2020-04-08T05:32:29Z

←Older revision		Revision as of 05:32, 8 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6w3o is ON HOLD	+	The entry 6w3o is ON HOLD until Paper Publication

	Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A.		Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A.

OCA: Protected "6w3o" [edit=sysop:move=sysop]

2020-03-18T08:32:36Z

Protected "6w3o" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:32, 18 March 2020

OCA: New page: '''Unreleased structure''' The entry 6w3o is ON HOLD Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A. Description: Crystal structure of ligand-binding domain of Campy...

2020-03-18T08:32:35Z

New page: '''Unreleased structure''' The entry 6w3o is ON HOLD Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A. Description: Crystal structure of ligand-binding domain of Campy...

New page

'''Unreleased structure'''

The entry 6w3o is ON HOLD

Authors: Khan, M.F., Machuca, M.A., Rahman, M.M., Roujeinikova, A.

Description: Crystal structure of ligand-binding domain of Campylobacter jejuni chemoreceptor Tlp3 in complex with 4-methylisoleucine
[[Category: Unreleased Structures]]
[[Category: Khan, M.F]]
[[Category: Roujeinikova, A]]
[[Category: Rahman, M.M]]
[[Category: Machuca, M.A]]