OCA at 19:29, 29 May 2024

2024-05-29T19:29:01Z

←Older revision		Revision as of 19:29, 29 May 2024
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-	====	+	==The Mycobacterium tuberculosis ClpB disaggregase hexamer structure with a locally refined N-terminal domain in the presence of DnaK chaperone and a model substrate==
-	<StructureSection load='6w6j' size='340' side='right'caption='[[6w6j]]' scene=''>	+	<StructureSection load='6w6j' size='340' side='right'caption='[[6w6j]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w6j]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W6J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W6J FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w6j OCA], [https://pdbe.org/6w6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w6j RCSB], [https://www.ebi.ac.uk/pdbsum/6w6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w6j ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w6j OCA], [https://pdbe.org/6w6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w6j RCSB], [https://www.ebi.ac.uk/pdbsum/6w6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w6j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CLPB_MYCTU CLPB_MYCTU] Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The M. tuberculosis (Mtb) ClpB is a protein disaggregase that helps to rejuvenate the bacterial cell. DnaK is a protein foldase that can function alone, but it can also bind to the ClpB hexamer to physically couple protein disaggregation with protein refolding, although the molecular mechanism is not well understood. Here, we report the cryo-EM analysis of the Mtb ClpB-DnaK bi-chaperone in the presence of ATPgammaS and a protein substrate. We observe three ClpB conformations in the presence of DnaK, identify a conserved TGIP loop linking the oligonucleotide/oligosaccharide-binding domain and the nucleotide-binding domain that is important for ClpB function, derive the interface between the regulatory middle domain of the ClpB and the DnaK nucleotide-binding domain, and find that DnaK binding stabilizes, but does not bend or tilt, the ClpB middle domain. We propose a model for the synergistic actions of aggregate dissolution and refolding by the Mtb ClpB-DnaK bi-chaperone system.
		+
		+	Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system.,Yin Y, Feng X, Yu H, Fay A, Kovach A, Glickman MS, Li H Cell Rep. 2021 May 25;35(8):109166. doi: 10.1016/j.celrep.2021.109166. PMID:34038719<ref>PMID:34038719</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6w6j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of ClpB\|3D structures of ClpB]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Li H]]
		+	[[Category: Yin Y]]

OCA at 09:15, 26 May 2021

2021-05-26T09:15:47Z

←Older revision		Revision as of 09:15, 26 May 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6w6j ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6w6j' size='340' side='right'caption='[[6w6j]]' scene=''>
-	~~Authors~~: ~~Yin, Y~~.Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~ClpB~~/~~DnaK complex~~, ~~Conformation T with NTD~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w6j OCA], [https://pdbe.org/6w6j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w6j RCSB], [https://www.ebi.ac.uk/pdbsum/6w6j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w6j ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yin, Y.Y~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:19, 15 April 2020

2020-04-15T05:19:58Z

←Older revision		Revision as of 05:19, 15 April 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6w6j is ON HOLD	+	The entry 6w6j is ON HOLD until Paper Publication

	Authors: Yin, Y.Y.		Authors: Yin, Y.Y.

OCA: Protected "6w6j" [edit=sysop:move=sysop]

2020-03-27T08:25:50Z

Protected "6w6j" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:25, 27 March 2020

OCA: New page: '''Unreleased structure''' The entry 6w6j is ON HOLD Authors: Yin, Y.Y. Description: ClpB/DnaK complex, Conformation T with NTD Category: Unreleased Structures [[Category: Yin, Y.Y...

2020-03-27T08:25:49Z

New page: '''Unreleased structure''' The entry 6w6j is ON HOLD Authors: Yin, Y.Y. Description: ClpB/DnaK complex, Conformation T with NTD Category: Unreleased Structures [[Category: Yin, Y.Y...

New page

'''Unreleased structure'''

The entry 6w6j is ON HOLD

Authors: Yin, Y.Y.

Description: ClpB/DnaK complex, Conformation T with NTD
[[Category: Unreleased Structures]]
[[Category: Yin, Y.Y]]

6w6j - Revision history

OCA at 19:29, 29 May 2024

OCA at 09:15, 26 May 2021

OCA at 05:19, 15 April 2020

OCA: Protected "6w6j" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6w6j is ON HOLD Authors: Yin, Y.Y. Description: ClpB/DnaK complex, Conformation T with NTD Category: Unreleased Structures [[Category: Yin, Y.Y...