6wuv - Revision history

OCA at 14:36, 18 October 2023

2023-10-18T14:36:44Z

←Older revision		Revision as of 14:36, 18 October 2023
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	<StructureSection load='6wuv' size='340' side='right'caption='[[6wuv]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='6wuv' size='340' side='right'caption='[[6wuv]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wuv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wuv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WUV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ELT:ethoxy~~-~~~{N}~~,~~~{N}-dimethyl-phosphonamidic+acid'>ELT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.63Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ACHE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ELT:ethoxy-~{N},~{N}-dimethyl-phosphonamidic+acid'>ELT</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wuv OCA], [https://pdbe.org/6wuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wuv RCSB], [https://www.ebi.ac.uk/pdbsum/6wuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wuv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wuv OCA], [https://pdbe.org/6wuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wuv RCSB], [https://www.ebi.ac.uk/pdbsum/6wuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wuv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACES_HUMAN ACES_HUMAN]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.<ref>PMID:2714437</ref> <ref>PMID:1748670</ref> <ref>PMID:1517212</ref> <ref>PMID:11985878</ref>	+	[https://www.uniprot.org/uniprot/ACES_HUMAN ACES_HUMAN] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.<ref>PMID:2714437</ref> <ref>PMID:1748670</ref> <ref>PMID:1517212</ref> <ref>PMID:11985878</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acetylcholinesterase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bester~~, S M~~]]	+	[[Category: Bester SM]]
-	[[Category: Height~~, J J~~]]	+	[[Category: Height JJ]]
-	[[Category: McGuire~~, J R~~]]	+	[[Category: McGuire JR]]
-	[[Category: Pegan~~, S D]]~~	+	[[Category: Pegan SD]]
-	~~[[Category: Ga]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nerve agent]]~~	+
-	~~[[Category: Tabun~~]]	+

OCA at 10:43, 31 March 2021

2021-03-31T10:43:20Z

←Older revision		Revision as of 10:43, 31 March 2021
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	==Crystal Structure of Recombinant Human Acetylcholinesterase Inhibited by GA==		==Crystal Structure of Recombinant Human Acetylcholinesterase Inhibited by GA==
-	<StructureSection load='6wuv' size='340' side='right'caption='[[6wuv]]' scene=''>	+	<StructureSection load='6wuv' size='340' side='right'caption='[[6wuv]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WUV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wuv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WUV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wuv OCA], [https://pdbe.org/6wuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wuv RCSB], [https://www.ebi.ac.uk/pdbsum/6wuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wuv ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ELT:ethoxy-~{N},~{N}-dimethyl-phosphonamidic+acid'>ELT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACHE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wuv OCA], [https://pdbe.org/6wuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wuv RCSB], [https://www.ebi.ac.uk/pdbsum/6wuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wuv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ACES_HUMAN ACES_HUMAN]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.<ref>PMID:2714437</ref> <ref>PMID:1748670</ref> <ref>PMID:1517212</ref> <ref>PMID:11985878</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recent use of organophosphate nerve agents in Syria, Malaysia, Russia, and the United Kingdom has reinforced the potential threat of their intentional release. These agents act through their ability to inhibit human acetylcholinesterase (hAChE; E.C. 3.1.1.7), an enzyme vital for survival. The toxicity of hAChE inhibition via G-series nerve agents has been demonstrated to vary widely depending on the G-agent used. To gain insight into this issue, the structures of hAChE inhibited by tabun, sarin, cyclosarin, soman, and GP were obtained along with the inhibition kinetics for these agents. Through this information, the role of hAChE active site plasticity in agent selectivity is revealed. With reports indicating that the efficacy of reactivators can vary based on the nerve agent inhibiting hAChE, human recombinatorially expressed hAChE was utilized to define these variations for HI-6 among various G-agents. To identify the structural underpinnings of this phenomenon, the structures of tabun, sarin, and soman-inhibited hAChE in complex with HI-6 were determined. This revealed how the presence of G-agent adducts impacts reactivator access and placement within the active site. These insights will contribute toward a path of next-generation reactivators and an improved understanding of the innate issues with the current reactivators.
		+
		+	Structural and Biochemical Insights into the Inhibition of Human Acetylcholinesterase by G-Series Nerve Agents and Subsequent Reactivation by HI-6.,McGuire JR, Bester SM, Guelta MA, Cheung J, Langley C, Winemiller MD, Bae SY, Funk V, Myslinski JM, Pegan SD, Height JJ Chem Res Toxicol. 2021 Feb 4. doi: 10.1021/acs.chemrestox.0c00406. PMID:33538594<ref>PMID:33538594</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wuv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acetylcholinesterase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bester SM]]	+	[[Category: Bester, S M]]
-	[[Category: Height JJ]]	+	[[Category: Height, J J]]
-	[[Category: McGuire JR]]	+	[[Category: McGuire, J R]]
-	[[Category: Pegan SD]]	+	[[Category: Pegan, S D]]
		+	[[Category: Ga]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nerve agent]]
		+	[[Category: Tabun]]

OCA at 09:56, 17 February 2021

2021-02-17T09:56:30Z

←Older revision		Revision as of 09:56, 17 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6wuv ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of Recombinant Human Acetylcholinesterase Inhibited by GA==
-		+	<StructureSection load='6wuv' size='340' side='right'caption='[[6wuv]]' scene=''>
-	~~Authors~~: ~~McGuire~~, J.R., ~~Bester~~, S.M., ~~Pegan, S~~.D., ~~Height, J~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WUV FirstGlance]. <br>
-	~~Description: Crystal Structure of Recombinant Human Acetylcholinesterase Inhibited by GA~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wuv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wuv OCA], [https://pdbe.org/6wuv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wuv RCSB], [https://www.ebi.ac.uk/pdbsum/6wuv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wuv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Pegan, S.D~~]]	+	__TOC__
-	[[Category: ~~Bester, S.M~~]]	+	</StructureSection>
-	[[Category: ~~Height, J.J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Mcguire, J.R~~]]	+	[[Category: Bester SM]]
		+	[[Category: Height JJ]]
		+	[[Category: McGuire JR]]
		+	[[Category: Pegan SD]]

OCA at 04:15, 20 May 2020

2020-05-20T04:15:59Z

←Older revision		Revision as of 04:15, 20 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wuv is ON HOLD	+	The entry 6wuv is ON HOLD until Paper Publication

	Authors: McGuire, J.R., Bester, S.M., Pegan, S.D., Height, J.J.		Authors: McGuire, J.R., Bester, S.M., Pegan, S.D., Height, J.J.

OCA: Protected "6wuv" [edit=sysop:move=sysop]

2020-05-13T04:09:56Z

Protected "6wuv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:09, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wuv is ON HOLD Authors: McGuire, J.R., Bester, S.M., Pegan, S.D., Height, J.J. Description: Crystal Structure of Recombinant Human Acetylcholinest...

2020-05-13T04:09:55Z

New page: '''Unreleased structure''' The entry 6wuv is ON HOLD Authors: McGuire, J.R., Bester, S.M., Pegan, S.D., Height, J.J. Description: Crystal Structure of Recombinant Human Acetylcholinest...

New page

'''Unreleased structure'''

The entry 6wuv is ON HOLD

Authors: McGuire, J.R., Bester, S.M., Pegan, S.D., Height, J.J.

Description: Crystal Structure of Recombinant Human Acetylcholinesterase Inhibited by GA
[[Category: Unreleased Structures]]
[[Category: Pegan, S.D]]
[[Category: Bester, S.M]]
[[Category: Height, J.J]]
[[Category: Mcguire, J.R]]