6wvj - Revision history

OCA at 19:29, 29 May 2024

2024-05-29T19:29:21Z

←Older revision		Revision as of 19:29, 29 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6wvj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis], [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WVJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6wvj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis], [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WVJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.36Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvj OCA], [https://pdbe.org/6wvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wvj RCSB], [https://www.ebi.ac.uk/pdbsum/6wvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvj OCA], [https://pdbe.org/6wvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wvj RCSB], [https://www.ebi.ac.uk/pdbsum/6wvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvj ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:19, 24 November 2022

2022-11-24T10:19:32Z

←Older revision		Revision as of 10:19, 24 November 2022
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-	====	+	==Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex==
-	<StructureSection load='6wvj' size='340' side='right'caption='[[6wvj]]' scene=''>	+	<StructureSection load='6wvj' size='340' side='right'caption='[[6wvj]], [[Resolution\|resolution]] 3.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wvj]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis], [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WVJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvj OCA], [~~http~~://pdbe.org/6wvj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wvj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wvj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvj ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvj OCA], [https://pdbe.org/6wvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wvj RCSB], [https://www.ebi.ac.uk/pdbsum/6wvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RPOA_BACSU RPOA_BACSU] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled complexes, but it was not known how it performed this function. Here, using single particle cryo-electron microscopy, we have determined the structures of Bacillus subtilis RNA polymerase (RNAP) elongation and HelD complexes, enabling analysis of the conformational changes that occur in RNAP driven by HelD interaction. HelD has a 2-armed structure which penetrates deep into the primary and secondary channels of RNA polymerase. One arm removes nucleic acids from the active site, and the other induces a large conformational change in the primary channel leading to removal and recycling of the stalled polymerase, representing a novel mechanism for recycling transcription complexes in bacteria.
		+
		+	Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD.,Newing TP, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, Tolun G, Lewis PJ Nat Commun. 2020 Dec 18;11(1):6420. doi: 10.1038/s41467-020-20157-5. PMID:33339820<ref>PMID:33339820</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wvj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Newing T]]
		+	[[Category: Oakley AJ]]
		+	[[Category: Tolun G]]

OCA at 09:37, 18 November 2020

2020-11-18T09:37:17Z

←Older revision		Revision as of 09:37, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6wvj ~~is ON HOLD~~	+	====
-		+	<StructureSection load='6wvj' size='340' side='right'caption='[[6wvj]]' scene=''>
-	~~Authors~~: ~~Newing, T~~.~~, Tolun, G~~.~~, Oakley, A~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Cryo-EM~~ structure ~~of Bacillus subtilis RNA Polymerase elongation complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvj OCA], [http://pdbe.org/6wvj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wvj RCSB], [http://www.ebi.ac.uk/pdbsum/6wvj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvj ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Oakley~~, A.J]]	+	__TOC__
-	[[Category: ~~Tolun, G~~]]	+	</StructureSection>
-	[[Category: ~~Newing, T~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:07, 9 September 2020

2020-09-09T07:07:07Z

←Older revision		Revision as of 07:07, 9 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wvj is ON HOLD ~~until Paper Publication~~	+	The entry 6wvj is ON HOLD

	Authors: Newing, T., Tolun, G., Oakley, A.J.		Authors: Newing, T., Tolun, G., Oakley, A.J.

OCA at 04:52, 10 June 2020

2020-06-10T04:52:56Z

←Older revision		Revision as of 04:52, 10 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wvj is ON HOLD	+	The entry 6wvj is ON HOLD until Paper Publication

	Authors: Newing, T., Tolun, G., Oakley, A.J.		Authors: Newing, T., Tolun, G., Oakley, A.J.
Line 7:		Line 7:
	Description: Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex		Description: Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Newing, T]]
	[[Category: Oakley, A.J]]		[[Category: Oakley, A.J]]
	[[Category: Tolun, G]]		[[Category: Tolun, G]]
		+	[[Category: Newing, T]]

OCA: Protected "6wvj" [edit=sysop:move=sysop]

2020-05-13T04:11:26Z

Protected "6wvj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wvj is ON HOLD Authors: Newing, T., Tolun, G., Oakley, A.J. Description: Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex ...

2020-05-13T04:11:24Z

New page: '''Unreleased structure''' The entry 6wvj is ON HOLD Authors: Newing, T., Tolun, G., Oakley, A.J. Description: Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex ...

New page

'''Unreleased structure'''

The entry 6wvj is ON HOLD

Authors: Newing, T., Tolun, G., Oakley, A.J.

Description: Cryo-EM structure of Bacillus subtilis RNA Polymerase elongation complex
[[Category: Unreleased Structures]]
[[Category: Newing, T]]
[[Category: Oakley, A.J]]
[[Category: Tolun, G]]