6x61 - Revision history

OCA at 14:44, 18 October 2023

2023-10-18T14:44:26Z

←Older revision		Revision as of 14:44, 18 October 2023
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-	====	+	==Crystal structure of the N-terminal thioredoxin domain of SasA in complex with the N-terminal CI domain of KaiC from Thermosynchococcus elongatus==
-	<StructureSection load='6x61' size='340' side='right'caption='[[6x61]]' scene=''>	+	<StructureSection load='6x61' size='340' side='right'caption='[[6x61]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x61]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X61 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x61 OCA], [https://pdbe.org/6x61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x61 RCSB], [https://www.ebi.ac.uk/pdbsum/6x61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x61 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x61 OCA], [https://pdbe.org/6x61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x61 RCSB], [https://www.ebi.ac.uk/pdbsum/6x61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x61 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KAIC_THEVB KAIC_THEVB] Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Circadian clocks control gene expression to provide an internal representation of local time. We report reconstitution of a complete cyanobacterial circadian clock in vitro, including the central oscillator, signal transduction pathways, downstream transcription factor, and promoter DNA. The entire system oscillates autonomously and remains phase coherent for many days with a fluorescence-based readout that enables real-time observation of each component simultaneously without user intervention. We identified the molecular basis for loss of cycling in an arrhythmic mutant and explored fundamental mechanisms of timekeeping in the cyanobacterial clock. We find that SasA, a circadian sensor histidine kinase associated with clock output, engages directly with KaiB on the KaiC hexamer to regulate period and amplitude of the central oscillator. SasA uses structural mimicry to cooperatively recruit the rare, fold-switched conformation of KaiB to the KaiC hexamer to form the nighttime repressive complex and enhance rhythmicity of the oscillator, particularly under limiting concentrations of KaiB. Thus, the expanded in vitro clock reveals previously unknown mechanisms by which the circadian system of cyanobacteria maintains the pace and rhythmicity under variable protein concentrations.
		+
		+	Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.,Chavan AG, Swan JA, Heisler J, Sancar C, Ernst DC, Fang M, Palacios JG, Spangler RK, Bagshaw CR, Tripathi S, Crosby P, Golden SS, Partch CL, LiWang A Science. 2021 Oct 8;374(6564):eabd4453. doi: 10.1126/science.abd4453. Epub 2021 , Oct 8. PMID:34618577<ref>PMID:34618577</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x61" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Circadian clock protein 3D structures\|Circadian clock protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Thermosynechococcus vestitus BP-1]]
		+	[[Category: Partch CL]]
		+	[[Category: Swan JA]]
		+	[[Category: Tripathi SM]]

OCA at 14:32, 2 June 2021

2021-06-02T14:32:29Z

←Older revision		Revision as of 14:32, 2 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6x61 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6x61' size='340' side='right'caption='[[6x61]]' scene=''>
-	~~Authors~~: ~~Swan, J~~.A.~~, Tripathi, S~~.M.~~, Partch, C~~.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of the N-terminal thioredoxin domain of SasA in complex with the N-terminal CI domain of KaiC from Thermosynchococcus elongatus~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x61 OCA], [https://pdbe.org/6x61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x61 RCSB], [https://www.ebi.ac.uk/pdbsum/6x61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x61 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Partch~~, C.L]]	+	__TOC__
-	[[Category: ~~Tripathi, S.M~~]]	+	</StructureSection>
-	[[Category: ~~Swan, J.A~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:41, 8 July 2020

2020-07-08T10:41:07Z

←Older revision		Revision as of 10:41, 8 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6x61 is ON HOLD	+	The entry 6x61 is ON HOLD until Paper Publication

	Authors: Swan, J.A., Tripathi, S.M., Partch, C.L.		Authors: Swan, J.A., Tripathi, S.M., Partch, C.L.

OCA: Protected "6x61" [edit=sysop:move=sysop]

2020-06-03T05:15:12Z

Protected "6x61" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:15, 3 June 2020

OCA: New page: '''Unreleased structure''' The entry 6x61 is ON HOLD Authors: Swan, J.A., Tripathi, S.M., Partch, C.L. Description: Crystal structure of the N-terminal thioredoxin domain of SasA in co...

2020-06-03T05:15:11Z

New page: '''Unreleased structure''' The entry 6x61 is ON HOLD Authors: Swan, J.A., Tripathi, S.M., Partch, C.L. Description: Crystal structure of the N-terminal thioredoxin domain of SasA in co...

New page

'''Unreleased structure'''

The entry 6x61 is ON HOLD

Authors: Swan, J.A., Tripathi, S.M., Partch, C.L.

Description: Crystal structure of the N-terminal thioredoxin domain of SasA in complex with the N-terminal CI domain of KaiC from Thermosynchococcus elongatus
[[Category: Unreleased Structures]]
[[Category: Partch, C.L]]
[[Category: Tripathi, S.M]]
[[Category: Swan, J.A]]