6xmf - Revision history

OCA at 07:00, 10 May 2023

2023-05-10T07:00:57Z

←Older revision		Revision as of 07:00, 10 May 2023
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-	====	+	==Cryo-EM structure of Cas12g binary complex==
-	<StructureSection load='6xmf' size='340' side='right'caption='[[6xmf]]' scene=''>	+	<StructureSection load='6xmf' size='340' side='right'caption='[[6xmf]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xmf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lachnospiraceae_bacterium_ND2006 Lachnospiraceae bacterium ND2006] and [https://en.wikipedia.org/wiki/Metagenome Metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XMF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmf OCA], [https://pdbe.org/6xmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xmf RCSB], [https://www.ebi.ac.uk/pdbsum/6xmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmf ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmf OCA], [https://pdbe.org/6xmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xmf RCSB], [https://www.ebi.ac.uk/pdbsum/6xmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cas12g, the type V-G CRISPR-Cas effector, is an RNA-guided ribonuclease that targets single-stranded RNA substrate. The CRISPR-Cas12g system offers a potential platform for transcriptome engineering and diagnostic applications. We determined the structures of Cas12g-guide RNA complexes in the absence and presence of target RNA by cryo-EM to a resolution of 3.1 A and 4.8 A, respectively. Cas12g adopts a bilobed structure with miniature REC2 and Nuc domains, whereas the guide RNAs fold into a flipped 'F' shape, which is primarily recognized by the REC lobe. Target RNA and the CRISPR RNA (crRNA) guide form a duplex that inserts into the central cavity between the REC and NUC lobes, inducing conformational changes in both lobes to activate Cas12g. The structural insights would facilitate the development of Cas12g-based applications.
		+
		+	Cryo-EM structure of the RNA-guided ribonuclease Cas12g.,Li Z, Zhang H, Xiao R, Han R, Chang L Nat Chem Biol. 2021 Apr;17(4):387-393. doi: 10.1038/s41589-020-00721-2. Epub 2021 , Jan 25. PMID:33495647<ref>PMID:33495647</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xmf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lachnospiraceae bacterium ND2006]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Metagenome]]
		+	[[Category: Chang L]]
		+	[[Category: Li Z]]
		+	[[Category: Zhang H]]

OCA at 11:16, 3 February 2021

2021-02-03T11:16:49Z

←Older revision		Revision as of 11:16, 3 February 2021
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	<StructureSection load='6xmf' size='340' side='right'caption='[[6xmf]]' scene=''>		<StructureSection load='6xmf' size='340' side='right'caption='[[6xmf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmf OCA], [~~http~~://pdbe.org/6xmf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xmf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xmf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmf ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmf OCA], [https://pdbe.org/6xmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xmf RCSB], [https://www.ebi.ac.uk/pdbsum/6xmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmf ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 05:50, 20 January 2021

2021-01-20T05:50:14Z

←Older revision		Revision as of 05:50, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6xmf ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6xmf' size='340' side='right'caption='[[6xmf]]' scene=''>
-	~~Authors~~: ~~Chang, L~~.~~, Li, Z~~.~~, Zhang, H~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Cryo~~-~~EM structure of~~ a ~~CRISPR-Cas complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xmf OCA], [http://pdbe.org/6xmf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xmf RCSB], [http://www.ebi.ac.uk/pdbsum/6xmf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xmf ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Zhang~~, H]]	+	__TOC__
-	[[Category: ~~Li, Z~~]]	+	</StructureSection>
-	[[Category: ~~Chang, L~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:51, 5 August 2020

2020-08-05T03:51:02Z

←Older revision		Revision as of 03:51, 5 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xmf is ON HOLD	+	The entry 6xmf is ON HOLD until Paper Publication

	Authors: Chang, L., Li, Z., Zhang, H.		Authors: Chang, L., Li, Z., Zhang, H.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Zhang, H]]		[[Category: Zhang, H]]
-	[[Category: Chang, L]]
	[[Category: Li, Z]]		[[Category: Li, Z]]
		+	[[Category: Chang, L]]

OCA: Protected "6xmf" [edit=sysop:move=sysop]

2020-07-08T10:58:57Z

Protected "6xmf" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:58, 8 July 2020

OCA: New page: '''Unreleased structure''' The entry 6xmf is ON HOLD Authors: Chang, L., Li, Z., Zhang, H. Description: Cryo-EM structure of a CRISPR-Cas complex Category: Unreleased Structures [[...

2020-07-08T10:58:56Z

New page: '''Unreleased structure''' The entry 6xmf is ON HOLD Authors: Chang, L., Li, Z., Zhang, H. Description: Cryo-EM structure of a CRISPR-Cas complex Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 6xmf is ON HOLD

Authors: Chang, L., Li, Z., Zhang, H.

Description: Cryo-EM structure of a CRISPR-Cas complex
[[Category: Unreleased Structures]]
[[Category: Zhang, H]]
[[Category: Chang, L]]
[[Category: Li, Z]]