OCA at 13:15, 24 January 2024

2024-01-24T13:15:38Z

←Older revision		Revision as of 13:15, 24 January 2024
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	==Crystal structure of the GH18 chitinase ChiB from the chitin utilization locus of Flavobacterium johnsoniae==		==Crystal structure of the GH18 chitinase ChiB from the chitin utilization locus of Flavobacterium johnsoniae==
-	<StructureSection load='6xyz' size='340' side='right'caption='[[6xyz]]' scene=''>	+	<StructureSection load='6xyz' size='340' side='right'caption='[[6xyz]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6XYZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6xyz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Flavobacterium_johnsoniae_UW101 Flavobacterium johnsoniae UW101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XYZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6xyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyz OCA], [~~http~~://pdbe.org/6xyz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6xyz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6xyz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyz OCA], [https://pdbe.org/6xyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xyz RCSB], [https://www.ebi.ac.uk/pdbsum/6xyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A5FB54_FLAJ1 A5FB54_FLAJ1]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chitin is one of the most abundant renewable organic materials found on earth. The chitin utilization locus in Flavobacterium johnsoniae, which encodes necessary proteins for complete enzymatic depolymerization of crystalline chitin, has recently been characterized but no detailed structural information on the enzymes was provided. Here we present protein structures of the F. johnsoniae chitobiase (FjGH20) and chitinase B (FjChiB). FjGH20 is a multi-domain enzyme with a helical domain not before observed in other chitobiases and a domain organization reminiscent of GH84 (beta-N-acetylglucosaminidase) family members. The structure of FjChiB reveals that the protein lacks loops and regions associated with exo-acting activity in other chitinases and instead has a more solvent accessible substrate binding cleft, which is consistent with its endo-chitinase activity. Additionally, small angle X-ray scattering data were collected for the internal 70 kDa region that connects the N- and C-terminal chitinase domains of the unique 158 kDa multi-domain chitinase A (FjChiA). The resulting model of the molecular envelope supports bioinformatic predictions of the region comprising six domains, each with similarities to either Fn3-like or Ig-like domains. Taken together, the results provide insights into chitin utilization by F. johnsoniae and reveal structural diversity in bacterial chitin metabolism.
		+
		+	Structural insights of the enzymes from the chitin utilization locus of Flavobacterium johnsoniae.,Mazurkewich S, Helland R, Mackenzie A, Eijsink VGH, Pope PB, Branden G, Larsbrink J Sci Rep. 2020 Aug 13;10(1):13775. doi: 10.1038/s41598-020-70749-w. PMID:32792608<ref>PMID:32792608</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6xyz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Flavobacterium johnsoniae UW101]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Branden G]]		[[Category: Branden G]]

OCA at 06:51, 2 September 2020

2020-09-02T06:51:31Z

←Older revision		Revision as of 06:51, 2 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6xyz is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the GH18 chitinase ChiB from the chitin utilization locus of Flavobacterium johnsoniae==
-		+	<StructureSection load='6xyz' size='340' side='right'caption='[[6xyz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XYZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6XYZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6xyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xyz OCA], [http://pdbe.org/6xyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xyz RCSB], [http://www.ebi.ac.uk/pdbsum/6xyz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xyz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Branden G]]
		+	[[Category: Eijsink V]]
		+	[[Category: Helland R]]
		+	[[Category: Larsbrink J]]
		+	[[Category: MacKenzie A]]
		+	[[Category: Mazurkewich S]]
		+	[[Category: Pope P]]

OCA at 07:06, 22 July 2020

2020-07-22T07:06:45Z

←Older revision		Revision as of 07:06, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6xyz is ON HOLD	+	The entry 6xyz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6xyz" [edit=sysop:move=sysop]

2020-02-13T02:52:21Z

Protected "6xyz" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:52, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6xyz is ON HOLD Authors: Description: Category: Unreleased Structures

2020-02-13T02:52:20Z

New page: '''Unreleased structure''' The entry 6xyz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6xyz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6xyz - Revision history

OCA at 13:15, 24 January 2024

OCA at 06:51, 2 September 2020

OCA at 07:06, 22 July 2020

OCA: Protected "6xyz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6xyz is ON HOLD Authors: Description: Category: Unreleased Structures