6yet - Revision history

OCA at 11:08, 14 June 2023

2023-06-14T11:08:18Z

←Older revision		Revision as of 11:08, 14 June 2023
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	==Second EH domain of AtEH1/Pan1==		==Second EH domain of AtEH1/Pan1==
-	<StructureSection load='6yet' size='340' side='right'caption='[[6yet~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6yet' size='340' side='right'caption='[[6yet]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6yet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Arath Arath~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YET FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g20760, F2D10.25, F2D10_25 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yet OCA], [https://pdbe.org/6yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yet RCSB], [https://www.ebi.ac.uk/pdbsum/6yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yet ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yet OCA], [https://pdbe.org/6yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yet RCSB], [https://www.ebi.ac.uk/pdbsum/6yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yet ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9LM78_ARATH Q9LM78_ARATH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Damme, D Van]]~~	+	[[Category: Evangelidis T]]
-	[[Category: Evangelidis, T]]	+	[[Category: Papageorgiou A]]
-	[[Category: Papageorgiou, A]]	+	[[Category: Tripsianes K]]
-	[[Category: Tripsianes, K]]	+	[[Category: Van Damme D]]
-	[[Category: ~~Yperman, K~~]]	+	[[Category: Yperman K]]
-	[[Category: ~~Adaptor protein]]~~	+
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Ef-hand]]~~	+
-	~~[[Category: Eh domain]]~~	+
-	~~[[Category: Lipid binding]]~~	+
-	~~[[Category: Lipid binding protein~~]]	+

OCA at 12:26, 9 June 2021

2021-06-09T12:26:25Z

←Older revision		Revision as of 12:26, 9 June 2021
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	==Second EH domain of AtEH1/Pan1==		==Second EH domain of AtEH1/Pan1==
-	<StructureSection load='6yet' size='340' side='right'caption='[[6yet]]' scene=''>	+	<StructureSection load='6yet' size='340' side='right'caption='[[6yet]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YET FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YET FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yet OCA], [https://pdbe.org/6yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yet RCSB], [https://www.ebi.ac.uk/pdbsum/6yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yet ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g20760, F2D10.25, F2D10_25 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yet OCA], [https://pdbe.org/6yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yet RCSB], [https://www.ebi.ac.uk/pdbsum/6yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yet ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clathrin-mediated endocytosis (CME) is the gatekeeper of the plasma membrane. In contrast to animals and yeasts, CME in plants depends on the TPLATE complex (TPC), an evolutionary ancient adaptor complex. However, the mechanistic contribution of the individual TPC subunits to plant CME remains elusive. In this study, we used a multidisciplinary approach to elucidate the structural and functional roles of the evolutionary conserved N-terminal Eps15 homology (EH) domains of the TPC subunit AtEH1/Pan1. By integrating high-resolution structural information obtained by X-ray crystallography and NMR spectroscopy with all-atom molecular dynamics simulations, we provide structural insight into the function of both EH domains. Both domains bind phosphatidic acid with a different strength, and only the second domain binds phosphatidylinositol 4,5-bisphosphate. Unbiased peptidome profiling by mass-spectrometry revealed that the first EH domain preferentially interacts with the double N-terminal NPF motif of a previously unidentified TPC interactor, the integral membrane protein Secretory Carrier Membrane Protein 5 (SCAMP5). Furthermore, we show that AtEH/Pan1 proteins control the internalization of SCAMP5 via this double NPF peptide interaction motif. Collectively, our structural and functional studies reveal distinct but complementary roles of the EH domains of AtEH/Pan1 in plant CME and connect the internalization of SCAMP5 to the TPLATE complex.
		+
		+	Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding.,Yperman K, Papageorgiou AC, Merceron R, De Munck S, Bloch Y, Eeckhout D, Jiang Q, Tack P, Grigoryan R, Evangelidis T, Van Leene J, Vincze L, Vandenabeele P, Vanhaecke F, Potocky M, De Jaeger G, Savvides SN, Tripsianes K, Pleskot R, Van Damme D Nat Commun. 2021 May 24;12(1):3050. doi: 10.1038/s41467-021-23314-6. PMID:34031427<ref>PMID:34031427</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yet" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Evangelidis T]]	+	[[Category: Damme, D Van]]
-	[[Category: Papageorgiou A]]	+	[[Category: Evangelidis, T]]
-	[[Category: Tripsianes K]]	+	[[Category: Papageorgiou, A]]
-	[[Category: ~~Van Damme D~~]]	+	[[Category: Tripsianes, K]]
-	[[Category: ~~Yperman K~~]]	+	[[Category: Yperman, K]]
		+	[[Category: Adaptor protein]]
		+	[[Category: Calcium binding]]
		+	[[Category: Ef-hand]]
		+	[[Category: Eh domain]]
		+	[[Category: Lipid binding]]
		+	[[Category: Lipid binding protein]]

OCA at 10:30, 31 March 2021

2021-03-31T10:30:14Z

←Older revision		Revision as of 10:30, 31 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6yet ~~is ON HOLD until Paper Publication~~	+	==Second EH domain of AtEH1/Pan1==
-		+	<StructureSection load='6yet' size='340' side='right'caption='[[6yet]]' scene=''>
-	~~Authors~~: ~~Yperman~~, K., ~~Papageorgiou, A~~., ~~Evangelidis, T~~., ~~Van Damme, D~~., ~~Tripsianes, K~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YET FirstGlance]. <br>
-	~~Description: Second EH domain of AtEH1~~/~~Pan1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yet OCA], [https://pdbe.org/6yet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yet RCSB], [https://www.ebi.ac.uk/pdbsum/6yet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yet ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Papageorgiou, A~~]]	+	__TOC__
-	[[Category: ~~Yperman, K~~]]	+	</StructureSection>
-	[[Category: Tripsianes, K]]	+	[[Category: Large Structures]]
-	[[Category: ~~Evangelidis, T~~]]	+	[[Category: Evangelidis T]]
-	[[Category: ~~Van Damme, D~~]]	+	[[Category: Papageorgiou A]]
		+	[[Category: Tripsianes K]]
		+	[[Category: Van Damme D]]
		+	[[Category: Yperman K]]

OCA at 07:20, 22 July 2020

2020-07-22T07:20:15Z

←Older revision		Revision as of 07:20, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6yet is ON HOLD	+	The entry 6yet is ON HOLD until Paper Publication

	Authors: Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K.		Authors: Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K.

OCA: Protected "6yet" [edit=sysop:move=sysop]

2020-04-01T07:45:51Z

Protected "6yet" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:45, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 6yet is ON HOLD Authors: Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K. Description: Second EH domain of AtEH1/Pan1 ...

2020-04-01T07:45:49Z

New page: '''Unreleased structure''' The entry 6yet is ON HOLD Authors: Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K. Description: Second EH domain of AtEH1/Pan1 ...

New page

'''Unreleased structure'''

The entry 6yet is ON HOLD

Authors: Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K.

Description: Second EH domain of AtEH1/Pan1
[[Category: Unreleased Structures]]
[[Category: Papageorgiou, A]]
[[Category: Yperman, K]]
[[Category: Tripsianes, K]]
[[Category: Evangelidis, T]]
[[Category: Van Damme, D]]