OCA at 08:24, 20 January 2021

2021-01-20T08:24:39Z

←Older revision		Revision as of 08:24, 20 January 2021
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	==Crystal structure of CNFy from Yersinia pseudotuberculosis - N-terminal fragment comprising residues 1-704==		==Crystal structure of CNFy from Yersinia pseudotuberculosis - N-terminal fragment comprising residues 1-704==
-	<StructureSection load='6yhl' size='340' side='right'caption='[[6yhl]]' scene=''>	+	<StructureSection load='6yhl' size='340' side='right'caption='[[6yhl]], [[Resolution\|resolution]] 3.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6yhl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pseudotuberkulosis"_(sic)_pfeiffer_1889 "bacillus pseudotuberkulosis" (sic) pfeiffer 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhl OCA], [http://pdbe.org/6yhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhl RCSB], [http://www.ebi.ac.uk/pdbsum/6yhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhl ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cnf ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 "Bacillus pseudotuberkulosis" (sic) Pfeiffer 1889])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhl OCA], [http://pdbe.org/6yhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhl RCSB], [http://www.ebi.ac.uk/pdbsum/6yhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytotoxic necrotizing factors (CNFs) are bacterial single-chain exotoxins that modulate cytokinetic/oncogenic and inflammatory processes through activation of host cell Rho GTPases. To achieve this, they are secreted, bind surface receptors to induce endocytosis and translocate a catalytic unit into the cytosol to intoxicate host cells. A three-dimensional structure that provides insight into the underlying mechanisms is still lacking. Here, we determined the crystal structure of full-length Yersinia pseudotuberculosis CNFY . CNFY consists of five domains (D1-D5), and by integrating structural and functional data, we demonstrate that D1-3 act as export and translocation module for the catalytic unit (D4-5) and for a fused beta-lactamase reporter protein. We further found that D4, which possesses structural similarity to ADP-ribosyl transferases, but had no equivalent catalytic activity, changed its position to interact extensively with D5 in the crystal structure of the free D4-5 fragment. This liberates D5 from a semi-blocked conformation in full-length CNFY , leading to higher deamidation activity. Finally, we identify CNF translocation modules in several uncharacterized fusion proteins, which suggests their usability as a broad-specificity protein delivery tool.
		+
		+	Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication.,Chaoprasid P, Lukat P, Muhlen S, Heidler T, Gazdag EM, Dong S, Bi W, Ruter C, Kirchenwitz M, Steffen A, Jansch L, Stradal TEB, Dersch P, Blankenfeldt W EMBO J. 2021 Jan 7:e105202. doi: 10.15252/embj.2020105202. PMID:33410511<ref>PMID:33410511</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6yhl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blankenfeldt W]]	+	[[Category: Blankenfeldt, W]]
-	[[Category: Gazdag EM]]	+	[[Category: Gazdag, E M]]
-	[[Category: Heidler TV]]	+	[[Category: Heidler, T V]]
-	[[Category: Lukat P]]	+	[[Category: Lukat, P]]
		+	[[Category: Cnf]]
		+	[[Category: Cytotoxic necrotizing factor]]
		+	[[Category: Deamidase]]
		+	[[Category: Putative adp-ribosyltransferase]]
		+	[[Category: Rhoa activation]]
		+	[[Category: Rhoa modification]]
		+	[[Category: Toxin]]

OCA at 06:45, 30 December 2020

2020-12-30T06:45:11Z

←Older revision		Revision as of 06:45, 30 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6yhl is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of CNFy from Yersinia pseudotuberculosis - N-terminal fragment comprising residues 1-704==
-		+	<StructureSection load='6yhl' size='340' side='right'caption='[[6yhl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhl OCA], [http://pdbe.org/6yhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhl RCSB], [http://www.ebi.ac.uk/pdbsum/6yhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Blankenfeldt W]]
		+	[[Category: Gazdag EM]]
		+	[[Category: Heidler TV]]
		+	[[Category: Lukat P]]

OCA: Protected "6yhl" [edit=sysop:move=sysop]

2020-04-08T05:43:49Z

Protected "6yhl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 8 April 2020

OCA: New page: '''Unreleased structure''' The entry 6yhl is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2020-04-08T05:43:48Z

New page: '''Unreleased structure''' The entry 6yhl is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6yhl is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6yhl - Revision history

OCA at 08:24, 20 January 2021

OCA at 06:45, 30 December 2020

OCA: Protected "6yhl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6yhl is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures