6ypc - Revision history

OCA at 13:32, 24 January 2024

2024-01-24T13:32:33Z

←Older revision		Revision as of 13:32, 24 January 2024
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-	====	+	==Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms==
-	<StructureSection load='6ypc' size='340' side='right'caption='[[6ypc]]' scene=''>	+	<StructureSection load='6ypc' size='340' side='right'caption='[[6ypc]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ypc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YPC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypc OCA], [https://pdbe.org/6ypc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypc RCSB], [https://www.ebi.ac.uk/pdbsum/6ypc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypc ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypc OCA], [https://pdbe.org/6ypc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypc RCSB], [https://www.ebi.ac.uk/pdbsum/6ypc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CENPK_YEAST CENPK_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.<ref>PMID:9987135</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kinetochores are large multi-subunit complexes that attach centromeric chromatin to microtubules of the mitotic spindle, enabling sister chromatid segregation in mitosis. The inner kinetochore constitutive centromere associated network (CCAN) complex assembles onto the centromere-specific Cenp-A nucleosome (Cenp-ANuc), thereby coupling the centromere to the microtubule-binding outer kinetochore. CCAN is a conserved 14-16 subunit complex composed of discrete modules. Here, we determined the crystal structure of the Saccharomyces cerevisiae Cenp-HIKHead-TW sub-module, revealing how Cenp-HIK and Cenp-TW interact at the conserved Cenp-HIKHead-Cenp-TW interface. A major interface is formed by the C-terminal anti-parallel alpha-helices of the histone fold extension (HFE) of the Cenp-T histone fold domain (HFD) combining with alpha-helix H3 of Cenp-K to create a compact three alpha-helical bundle. We fitted the Cenp-HIKHead-TW sub-module to the previously determined cryo-EM map of the S. cerevisiae CCAN-Cenp-ANuc complex. This showed that the HEAT repeat domain of Cenp-IHead and C-terminal HFD of Cenp-T of the Cenp-HIKHead-TW sub-module interact with the nucleosome DNA gyre at a site close to the Cenp-ANuc dyad axis. Our structure provides a framework for understanding how Cenp-T links centromeric Cenp-ANuc to the outer kinetochore through its HFD and N-terminal Ndc80-binding motif, respectively.
		+
		+	Crystal structure of the Cenp-HIKHead-TW sub-module of the inner kinetochore CCAN complex.,Zhang Z, Bellini D, Barford D Nucleic Acids Res. 2020 Nov 4;48(19):11172-11184. doi: 10.1093/nar/gkaa772. PMID:32976599<ref>PMID:32976599</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ypc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Barford D]]
		+	[[Category: Bellini D]]
		+	[[Category: Zhang Z]]

OCA at 14:59, 3 March 2021

2021-03-03T14:59:18Z

←Older revision		Revision as of 14:59, 3 March 2021
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	<StructureSection load='6ypc' size='340' side='right'caption='[[6ypc]]' scene=''>		<StructureSection load='6ypc' size='340' side='right'caption='[[6ypc]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ypc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypc OCA], [~~http~~://pdbe.org/6ypc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ypc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ypc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypc ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypc OCA], [https://pdbe.org/6ypc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypc RCSB], [https://www.ebi.ac.uk/pdbsum/6ypc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypc ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 09:50, 16 September 2020

2020-09-16T09:50:57Z

←Older revision		Revision as of 09:50, 16 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ypc ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6ypc' size='340' side='right'caption='[[6ypc]]' scene=''>
-	~~Authors~~: ~~Bellini, D~~.~~, Zhang, Z~~.~~, Barford, D~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of~~ the ~~kinetochore subunits H~~/I/K/T/~~W penta-complex from S~~. ~~cerevisiae at 2~~.~~9 angstroms~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ypc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypc OCA], [http://pdbe.org/6ypc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ypc RCSB], [http://www.ebi.ac.uk/pdbsum/6ypc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypc ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bellini~~, D]]	+	__TOC__
-	[[Category: ~~Barford, D~~]]	+	</StructureSection>
-	[[Category: ~~Zhang,~~ Z]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 03:53, 5 August 2020

2020-08-05T03:53:32Z

←Older revision		Revision as of 03:53, 5 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ypc is ON HOLD	+	The entry 6ypc is ON HOLD until Paper Publication

	Authors: Bellini, D., Zhang, Z., Barford, D.		Authors: Bellini, D., Zhang, Z., Barford, D.
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	Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms		Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Bellini, D]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Zhang, Z]]		[[Category: Zhang, Z]]
-	[[Category: Bellini, D]]

OCA at 07:31, 22 July 2020

2020-07-22T07:31:11Z

←Older revision		Revision as of 07:31, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ypc is ON HOLD ~~until Paper Publication~~	+	The entry 6ypc is ON HOLD

	Authors: Bellini, D., Zhang, Z., Barford, D.		Authors: Bellini, D., Zhang, Z., Barford, D.
Line 7:		Line 7:
	Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms		Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zhang, Z]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
		+	[[Category: Zhang, Z]]
	[[Category: Bellini, D]]		[[Category: Bellini, D]]

OCA at 05:24, 29 April 2020

2020-04-29T05:24:00Z

←Older revision		Revision as of 05:24, 29 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ypc is ON HOLD	+	The entry 6ypc is ON HOLD until Paper Publication

	Authors: Bellini, D., Zhang, Z., Barford, D.		Authors: Bellini, D., Zhang, Z., Barford, D.
Line 7:		Line 7:
	Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms		Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Zhang, Z]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Bellini, D]]		[[Category: Bellini, D]]
-	[[Category: Zhang, Z]]

OCA: Protected "6ypc" [edit=sysop:move=sysop]

2020-04-22T04:31:03Z

Protected "6ypc" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:31, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 6ypc is ON HOLD Authors: Bellini, D., Zhang, Z., Barford, D. Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex fro...

2020-04-22T04:31:01Z

New page: '''Unreleased structure''' The entry 6ypc is ON HOLD Authors: Bellini, D., Zhang, Z., Barford, D. Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex fro...

New page

'''Unreleased structure'''

The entry 6ypc is ON HOLD

Authors: Bellini, D., Zhang, Z., Barford, D.

Description: Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms
[[Category: Unreleased Structures]]
[[Category: Barford, D]]
[[Category: Bellini, D]]
[[Category: Zhang, Z]]