6ytc - Revision history

OCA at 11:08, 14 June 2023

2023-06-14T11:08:34Z

←Older revision		Revision as of 11:08, 14 June 2023
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	==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter==		==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter==
-	<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ytc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_thetaiotaomicron"_distaso_1912 "bacillus~~ thetaiotaomicron~~" distaso 1912~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ytc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF2534_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [https://pdbe.org/6ytc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [https://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [~~http~~://pdbe.org/6ytc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8A6W3_BACTN Q8A6W3_BACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ thetaiotaomicron ~~distaso 1912~~]]	+	[[Category: Bacteroides thetaiotaomicron]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hiller, S]]	+	[[Category: Hiller S]]
-	[[Category: Mazur, A]]	+	[[Category: Mazur A]]
-	[[Category: Rath, P]]	+	[[Category: Rath P]]
-	~~[[Category: Glycan import]]~~	+
-	~~[[Category: Ig-like fold]]~~	+
-	~~[[Category: N-terminal domain]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Transporter~~]]	+

OCA at 08:25, 20 January 2021

2021-01-20T08:25:17Z

←Older revision		Revision as of 08:25, 20 January 2021
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	==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter==		==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter==
-	<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]]' scene=''>	+	<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ytc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_thetaiotaomicron"_distaso_1912 "bacillus thetaiotaomicron" distaso 1912]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [http://pdbe.org/6ytc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [http://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HMPREF2534_01945 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [http://pdbe.org/6ytc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [http://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the beta2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism.
		+
		+	Insights into SusCD-mediated glycan import by a prominent gut symbiont.,Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B Nat Commun. 2021 Jan 4;12(1):44. doi: 10.1038/s41467-020-20285-y. PMID:33398001<ref>PMID:33398001</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ytc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus thetaiotaomicron distaso 1912]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hiller S]]	+	[[Category: Hiller, S]]
-	[[Category: Mazur A]]	+	[[Category: Mazur, A]]
-	[[Category: Rath P]]	+	[[Category: Rath, P]]
		+	[[Category: Glycan import]]
		+	[[Category: Ig-like fold]]
		+	[[Category: N-terminal domain]]
		+	[[Category: Protein transport]]
		+	[[Category: Transporter]]

OCA at 14:12, 8 July 2020

2020-07-08T14:12:11Z

←Older revision		Revision as of 14:12, 8 July 2020
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-	'''Unreleased structure'''

-	~~The entry 6ytc is ON HOLD~~	+	==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter==
-		+	<StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]]' scene=''>
-	~~Authors: Rath, P., Mazur, A., Hiller, S.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br>
-	~~Description:~~ Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [http://pdbe.org/6ytc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [http://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Hiller, S]]	+	__TOC__
-	[[Category: ~~Rath, P~~]]	+	</StructureSection>
-	[[Category: ~~Mazur, A~~]]	+	[[Category: Large Structures]]
		+	[[Category: Hiller S]]
		+	[[Category: Mazur A]]
		+	[[Category: Rath P]]

OCA: Protected "6ytc" [edit=sysop:move=sysop]

2020-04-29T05:32:51Z

Protected "6ytc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 6ytc is ON HOLD Authors: Rath, P., Mazur, A., Hiller, S. Description: Solution NMR structure of the isolated NTE domain of BT1762-63 levan transpor...

2020-04-29T05:32:49Z

New page: '''Unreleased structure''' The entry 6ytc is ON HOLD Authors: Rath, P., Mazur, A., Hiller, S. Description: Solution NMR structure of the isolated NTE domain of BT1762-63 levan transpor...

New page

'''Unreleased structure'''

The entry 6ytc is ON HOLD

Authors: Rath, P., Mazur, A., Hiller, S.

Description: Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter
[[Category: Unreleased Structures]]
[[Category: Hiller, S]]
[[Category: Rath, P]]
[[Category: Mazur, A]]