6zxu - Revision history

OCA at 12:00, 1 February 2024

2024-02-01T12:00:01Z

←Older revision		Revision as of 12:00, 1 February 2024
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	==Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.==		==Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.==
-	<StructureSection load='6zxu' size='340' side='right'caption='[[6zxu]]' scene=''>	+	<StructureSection load='6zxu' size='340' side='right'caption='[[6zxu]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6ZXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6zxu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitratireductor_pacificus_pht-3B Nitratireductor pacificus pht-3B]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZXU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxu OCA], [~~http~~://pdbe.org/6zxu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6zxu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6zxu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.73Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxu OCA], [https://pdbe.org/6zxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zxu RCSB], [https://www.ebi.ac.uk/pdbsum/6zxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/K2MB66_9HYPH K2MB66_9HYPH]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Reductive dehalogenases are responsible for the reductive cleavage of carbon-halogen bonds during organohalide respiration. A variety of mechanisms have been proposed for these cobalamin and [4Fe-4S] containing enzymes, including organocobalt, radical, or cobalt-halide adduct based catalysis. The latter was proposed for the oxygen-tolerant Nitratireductor pacificus pht-3B catabolic reductive dehalogenase (NpRdhA). Here, we present the first substrate bound NpRdhA crystal structures, confirming a direct cobalt-halogen interaction is established and providing a rationale for substrate preference. Product formation is observed in crystallo due to X-ray photoreduction. Protein engineering enables rational alteration of substrate preference, providing a future blue print for the application of this and related enzymes in bioremediation.
		+
		+	Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope.,Halliwell T, Fisher K, Payne KAP, Rigby SEJ, Leys D Microorganisms. 2020 Sep 2;8(9). pii: microorganisms8091344. doi:, 10.3390/microorganisms8091344. PMID:32887524<ref>PMID:32887524</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6zxu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Nitratireductor pacificus pht-3B]]
	[[Category: Halliwell T]]		[[Category: Halliwell T]]
	[[Category: Leys D]]		[[Category: Leys D]]

OCA at 09:54, 16 September 2020

2020-09-16T09:54:35Z

←Older revision		Revision as of 09:54, 16 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6zxu ~~is ON HOLD~~	+	==Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.==
-		+	<StructureSection load='6zxu' size='340' side='right'caption='[[6zxu]]' scene=''>
-	~~Authors~~: ~~Leys~~, D., ~~Halliwell~~, T.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZXU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ZXU FirstGlance]. <br>
-	~~Description~~: ~~Catabolic reductive dehalogenase NpRdhA~~, N-~~terminally tagged~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6zxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zxu OCA], [http://pdbe.org/6zxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6zxu RCSB], [http://www.ebi.ac.uk/pdbsum/6zxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6zxu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Leys, D~~]]	+	__TOC__
-	[[Category: ~~Halliwell, T~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Halliwell T]]
		+	[[Category: Leys D]]

OCA: Protected "6zxu" [edit=sysop:move=sysop]

2020-08-05T04:04:31Z

Protected "6zxu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:04, 5 August 2020

OCA: New page: '''Unreleased structure''' The entry 6zxu is ON HOLD Authors: Leys, D., Halliwell, T. Description: Catabolic reductive dehalogenase NpRdhA, N-terminally tagged. [[Category: Unreleased ...

2020-08-05T04:04:29Z

New page: '''Unreleased structure''' The entry 6zxu is ON HOLD Authors: Leys, D., Halliwell, T. Description: Catabolic reductive dehalogenase NpRdhA, N-terminally tagged. [[Category: Unreleased ...

New page

'''Unreleased structure'''

The entry 6zxu is ON HOLD

Authors: Leys, D., Halliwell, T.

Description: Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.
[[Category: Unreleased Structures]]
[[Category: Leys, D]]
[[Category: Halliwell, T]]