OCA at 08:32, 23 February 2022

2022-02-23T08:32:53Z

←Older revision		Revision as of 08:32, 23 February 2022
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	==Structure of homodimeric actin capping protein alpha subunit from Plasmodium berghei==		==Structure of homodimeric actin capping protein alpha subunit from Plasmodium berghei==
-	<StructureSection load='7a0h' size='340' side='right'caption='[[7a0h]]' scene=''>	+	<StructureSection load='7a0h' size='340' side='right'caption='[[7a0h]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a0h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plaba Plaba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0H FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0h OCA], [https://pdbe.org/7a0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0h RCSB], [https://www.ebi.ac.uk/pdbsum/7a0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0h ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PBANKA_1243100 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5823 PLABA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0h OCA], [https://pdbe.org/7a0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0h RCSB], [https://www.ebi.ac.uk/pdbsum/7a0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0h ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A509AR49_PLABA A0A509AR49_PLABA]] F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.[RuleBase:RU365077]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Apicomplexan parasites, such as Plasmodium spp., rely on an unusual actomyosin motor, termed glideosome, for motility and host cell invasion. The actin filaments are maintained by a small set of essential regulators, which provide control over actin dynamics in the different stages of the parasite life cycle. Actin filament capping proteins (CPs) are indispensable heterodimeric regulators of actin dynamics. CPs have been extensively characterized in higher eukaryotes, but their role and functional mechanism in Apicomplexa remain enigmatic. Here, we present the first crystal structure of a homodimeric CP from the malaria parasite and compare the homo- and heterodimeric CP structures in detail. Despite retaining several characteristics of a canonical CP, the homodimeric Plasmodium berghei (Pb)CP exhibits crucial differences to the canonical heterodimers. Both homo- and heterodimeric PbCPs regulate actin dynamics in an atypical manner, facilitating rapid turnover of parasite actin, without affecting its critical concentration. Homo- and heterodimeric PbCPs show partially redundant activities, possibly to rescue actin filament capping in life cycle stages where the beta-subunit is downregulated. Our data suggest that the homodimeric PbCP also influences actin kinetics by recruiting lateral actin dimers. This unusual function could arise from the absence of a beta-subunit, as the asymmetric PbCP homodimer lacks structural elements essential for canonical barbed end interactions suggesting a novel CP binding mode. These findings will facilitate further studies aimed at elucidating the precise actin filament capping mechanism in Plasmodium.
		+
		+	Structure and function of an atypical homodimeric actin capping protein from the malaria parasite.,Bendes AA, Kursula P, Kursula I Cell Mol Life Sci. 2022 Feb 8;79(2):125. doi: 10.1007/s00018-021-04032-0. PMID:35132495<ref>PMID:35132495</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a0h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bendes AA]]	+	[[Category: Plaba]]
-	[[Category: Kursula I]]	+	[[Category: Bendes, A A]]
-	[[Category: Kursula P]]	+	[[Category: Kursula, I]]
		+	[[Category: Kursula, P]]
		+	[[Category: Asymmetric homodimer]]
		+	[[Category: Capping protein]]
		+	[[Category: F-actin binding]]
		+	[[Category: Protein binding]]

OCA at 06:06, 18 August 2021

2021-08-18T06:06:29Z

←Older revision		Revision as of 06:06, 18 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7a0h is ~~ON HOLD until Paper Publication~~	+	==Structure of homodimeric actin capping protein alpha subunit from Plasmodium berghei==
-		+	<StructureSection load='7a0h' size='340' side='right'caption='[[7a0h]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A0H FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a0h OCA], [https://pdbe.org/7a0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a0h RCSB], [https://www.ebi.ac.uk/pdbsum/7a0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a0h ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bendes AA]]
		+	[[Category: Kursula I]]
		+	[[Category: Kursula P]]

OCA at 07:11, 9 September 2020

2020-09-09T07:11:27Z

←Older revision		Revision as of 07:11, 9 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a0h is ON HOLD	+	The entry 7a0h is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a0h" [edit=sysop:move=sysop]

2020-08-19T04:36:37Z

Protected "7a0h" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a0h is ON HOLD Authors: Description: Category: Unreleased Structures

2020-08-19T04:36:35Z

New page: '''Unreleased structure''' The entry 7a0h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7a0h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7a0h - Revision history

OCA at 08:32, 23 February 2022

OCA at 06:06, 18 August 2021

OCA at 07:11, 9 September 2020

OCA: Protected "7a0h" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7a0h is ON HOLD Authors: Description: Category: Unreleased Structures