OCA at 12:01, 1 February 2024

2024-02-01T12:01:53Z

←Older revision		Revision as of 12:01, 1 February 2024
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-	====	+	==Crystal structure of the 5-phosphatase domain of Synaptojanin1 bound to its substrate diC8-PI(3,4,5)P3 in complex with a nanobody==
-	<StructureSection load='7a17' size='340' side='right'caption='[[7a17]]' scene=''>	+	<StructureSection load='7a17' size='340' side='right'caption='[[7a17]], [[Resolution\|resolution]] 2.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a17]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A17 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7a17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a17 OCA], [~~http~~://pdbe.org/7a17 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7a17 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7a17 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7a17 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.73Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IP9:(2R)-3-{[(R)-{[(1S,2S,3R,4S,5S,6S)-2,6-DIHYDROXY-3,4,5-TRIS(PHOSPHONOOXY)CYCLOHEXYL]OXY}(HYDROXY)PHOSPHORYL]OXY}PROPANE-1,2-DIYL+DIOCTANOATE'>IP9</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a17 OCA], [https://pdbe.org/7a17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a17 RCSB], [https://www.ebi.ac.uk/pdbsum/7a17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a17 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SYNJ1_HUMAN SYNJ1_HUMAN] Inositol 5-phosphatase which has a role in clathrin-mediated endocytosis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Synaptojanin1 (Synj1) is a phosphoinositide phosphatase, important in clathrin uncoating during endocytosis of presynaptic vesicles. It was identified as a potential drug target for Alzheimer's disease, Down syndrome, and TBC1D24-associated epilepsy, while also loss-of-function mutations in Synj1 are associated with epilepsy and Parkinson's disease. Despite its involvement in a range of disorders, structural, and detailed mechanistic information regarding the enzyme is lacking. Here, we report the crystal structure of the 5-phosphatase domain of Synj1. Moreover, we also present a structure of this domain bound to the substrate diC8-PI(3,4,5)P(3), providing the first image of a 5-phosphatase with a trapped substrate in its active site. Together with an analysis of the contribution of the different inositide phosphate groups to catalysis, these structures provide new insights in the Synj1 mechanism. Finally, we analysed the effect of three clinical missense mutations (Y793C, R800C, Y849C) on catalysis, unveiling the molecular mechanisms underlying Synj1-associated disease.
		+
		+	A structure of substrate-bound Synaptojanin1 provides new insights in its mechanism and the effect of disease mutations.,Paesmans J, Martin E, Deckers B, Berghmans M, Sethi R, Loeys Y, Pardon E, Steyaert J, Verstreken P, Galicia C, Versees W Elife. 2020 Dec 22;9:e64922. doi: 10.7554/eLife.64922. PMID:33349335<ref>PMID:33349335</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a17" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Galicia C]]
		+	[[Category: Martin E]]
		+	[[Category: Paesmans J]]
		+	[[Category: Versees W]]

OCA at 06:46, 30 December 2020

2020-12-30T06:46:20Z

←Older revision		Revision as of 06:46, 30 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7a17 ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7a17' size='340' side='right'caption='[[7a17]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7a17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a17 OCA], [http://pdbe.org/7a17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7a17 RCSB], [http://www.ebi.ac.uk/pdbsum/7a17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7a17 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:32, 24 December 2020

2020-12-24T07:32:39Z

←Older revision		Revision as of 07:32, 24 December 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a17 is ON HOLD ~~until Paper Publication~~	+	The entry 7a17 is ON HOLD

	Authors:		Authors:

OCA at 04:56, 9 December 2020

2020-12-09T04:56:04Z

←Older revision		Revision as of 04:56, 9 December 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a17 is ON HOLD	+	The entry 7a17 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7a17" [edit=sysop:move=sysop]

2020-08-19T04:38:27Z

Protected "7a17" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:38, 19 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a17 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-08-19T04:38:25Z

New page: '''Unreleased structure''' The entry 7a17 is ON HOLD Authors: Description: Category: Unreleased Structures

New page