7a3z - Revision history

OCA at 12:03, 1 February 2024

2024-02-01T12:03:24Z

←Older revision		Revision as of 12:03, 1 February 2024
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	==OSM-3 kinesin motor domain complexed with Mg.ADP==		==OSM-3 kinesin motor domain complexed with Mg.ADP==
-	<StructureSection load='7a3z' size='340' side='right'caption='[[7a3z]]' scene=''>	+	<StructureSection load='7a3z' size='340' side='right'caption='[[7a3z]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a3z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3Z FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3z OCA], [https://pdbe.org/7a3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3z RCSB], [https://www.ebi.ac.uk/pdbsum/7a3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3z ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.095Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3z OCA], [https://pdbe.org/7a3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3z RCSB], [https://www.ebi.ac.uk/pdbsum/7a3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3z ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/OSM3_CAEEL OSM3_CAEEL] Kinesin motor protein which is required for the anterograde intraflagellar transport (IFT) along the middle segment of the sensory neuron cilia together with the kinesin II motor complex (composed of klp-11, klp-20 and kap-1) and on its own, is required for IFT along the distal segment (PubMed:17000880, PubMed:17420466). In addition, regulates the length of cilia (PubMed:17420466). May have a role during neurogenesis and axonal transport (PubMed:7714894, PubMed:7690265).<ref>PMID:17000880</ref> <ref>PMID:17420466</ref> <ref>PMID:7690265</ref> <ref>PMID:7714894</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Motile kinesins are motor proteins that translocate along microtubules as they hydrolyze ATP. They share a conserved motor domain which harbors both ATPase and microtubule-binding activities. An ATP hydrolysis mechanism involving two water molecules has been proposed based on the structure of the kinesin-5 Eg5 bound to an ATP analog. Whether this mechanism is general in the kinesin superfamily remains uncertain. Here, we present structural snapshots of the motor domain of OSM-3 along its nucleotide cycle. OSM-3 belongs to the homodimeric kinesin-2 subfamily and is the Caenorhabditis elegans homologue of human KIF17. OSM-3 bound to ADP or devoid of a nucleotide shows features of ADP-kinesins with a docked neck linker. When bound to an ATP analog, OSM-3 adopts a conformation similar to those of several ATP-like kinesins, either isolated or bound to tubulin. Moreover, the OSM-3 nucleotide-binding site is virtually identical to that of ATP-like Eg5, demonstrating a shared ATPase mechanism. Therefore, our data extend to kinesin-2 the two-water ATP hydrolysis mechanism and further suggest that it is universal within the kinesin superfamily. PROTEIN DATABASE ENTRIES: 7A3Z, 7A40, 7A5E.
		+
		+	Structural snapshots of the kinesin-2 OSM-3 along its nucleotide cycle: implications for the ATP hydrolysis mechanism.,Varela PF, Chenon M, Velours C, Verhey KJ, Menetrey J, Gigant B FEBS Open Bio. 2021 Mar;11(3):564-577. doi: 10.1002/2211-5463.13101. Epub 2021 , Feb 28. PMID:33513284<ref>PMID:33513284</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a3z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gigant B]]		[[Category: Gigant B]]
	[[Category: Menetrey J]]		[[Category: Menetrey J]]
	[[Category: Varela FP]]		[[Category: Varela FP]]

OCA at 11:09, 3 February 2021

2021-02-03T11:09:10Z

←Older revision		Revision as of 11:09, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7a3z ~~is ON HOLD until Paper Publication~~	+	==OSM-3 kinesin motor domain complexed with Mg.ADP==
-		+	<StructureSection load='7a3z' size='340' side='right'caption='[[7a3z]]' scene=''>
-	~~Authors~~: ~~Varela~~, F.P., ~~Menetrey~~, J., ~~Gigant, B~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A3Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A3Z FirstGlance]. <br>
-	~~Description~~: ~~OSM~~-~~3 kinesin motor domain complexed with Mg~~.~~ADP~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a3z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a3z OCA], [https://pdbe.org/7a3z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a3z RCSB], [https://www.ebi.ac.uk/pdbsum/7a3z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a3z ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Gigant, B]]	+	__TOC__
-	[[Category: Menetrey, J]]	+	</StructureSection>
-	[[Category: Varela~~, F.P~~]]	+	[[Category: Large Structures]]
		+	[[Category: Gigant B]]
		+	[[Category: Menetrey J]]
		+	[[Category: Varela FP]]

OCA at 06:39, 16 September 2020

2020-09-16T06:39:02Z

←Older revision		Revision as of 06:39, 16 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a3z is ON HOLD	+	The entry 7a3z is ON HOLD until Paper Publication

	Authors: Varela, F.P., Menetrey, J., Gigant, B.		Authors: Varela, F.P., Menetrey, J., Gigant, B.
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	Description: OSM-3 kinesin motor domain complexed with Mg.ADP		Description: OSM-3 kinesin motor domain complexed with Mg.ADP
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Menetrey, J]]
	[[Category: Gigant, B]]		[[Category: Gigant, B]]
		+	[[Category: Menetrey, J]]
	[[Category: Varela, F.P]]		[[Category: Varela, F.P]]

OCA: Protected "7a3z" [edit=sysop:move=sysop]

2020-08-26T09:16:43Z

Protected "7a3z" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:16, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a3z is ON HOLD Authors: Varela, F.P., Menetrey, J., Gigant, B. Description: OSM-3 kinesin motor domain complexed with Mg.ADP [[Category: Unrelease...

2020-08-26T09:16:42Z

New page: '''Unreleased structure''' The entry 7a3z is ON HOLD Authors: Varela, F.P., Menetrey, J., Gigant, B. Description: OSM-3 kinesin motor domain complexed with Mg.ADP [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 7a3z is ON HOLD

Authors: Varela, F.P., Menetrey, J., Gigant, B.

Description: OSM-3 kinesin motor domain complexed with Mg.ADP
[[Category: Unreleased Structures]]
[[Category: Menetrey, J]]
[[Category: Gigant, B]]
[[Category: Varela, F.P]]