7a58 - Revision history

OCA at 09:37, 12 May 2021

2021-05-12T09:37:24Z

←Older revision		Revision as of 09:37, 12 May 2021
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	==NOE-only solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==		==NOE-only solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==
-	<StructureSection load='7a58' size='340' side='right'caption='[[7a58]]' scene=''>	+	<StructureSection load='7a58' size='340' side='right'caption='[[7a58]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A58 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7A58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7a58]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhopt Rhopt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A58 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a58 OCA], [~~http~~://pdbe.org/7a58 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7a58 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7a58 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7a58 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6xyv\|6xyv]], [[7a4l\|7a4l]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rpal_0815 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395960 RHOPT])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a58 OCA], [https://pdbe.org/7a58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a58 RCSB], [https://www.ebi.ac.uk/pdbsum/7a58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a58 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metalloproteins play key roles across biology, and knowledge of their structure is essential to understand their physiological role. For those metalloproteins containing paramagnetic states, the enhanced relaxation caused by the unpaired electrons often makes signal detection unfeasible near the metal center, precluding adequate structural characterization right where it is more biochemically relevant. Here, we report a protein structure determination by NMR where two different sets of restraints, one containing Nuclear Overhauser Enhancements (NOEs) and another containing Paramagnetic Relaxation Enhancements (PREs), are used separately and eventually together. The protein PioC from Rhodopseudomonas palustris TIE-1 is a High Potential Iron-Sulfur Protein (HiPIP) where the [4Fe-4S] cluster is paramagnetic in both oxidation states at room temperature providing the source of PREs used as alternative distance restraints. Comparison of the family of structures obtained using NOEs only, PREs only and the combination of both reveals that the pairwise root-mean-square deviation (RMSD) between them are similar and comparable with the precision within each family. This demonstrates that, under favorable conditions in terms of protein size and paramagnetic effects, PREs can efficiently complement and eventually replace NOEs for the structural characterization of small paramagnetic metalloproteins and denovo designed metalloproteins by NMR.
		+
		+	PRE-driven Protein NMR Structures: an Alternative Approach in Highly Paramagnetic Systems.,Trindade IB, Invernici M, Cantini F, Louro RO, Piccioli M FEBS J. 2020 Oct 30. doi: 10.1111/febs.15615. PMID:33124176<ref>PMID:33124176</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7a58" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cantini F]]	+	[[Category: Rhopt]]
-	[[Category: Trindade I]]	+	[[Category: Cantini, F]]
		+	[[Category: Trindade, I]]
		+	[[Category: Electron transport]]
		+	[[Category: Iron-sulphur protein]]
		+	[[Category: Metallo protein]]
		+	[[Category: Paramagnetic nmr]]
		+	[[Category: Paramagnetic protein]]
		+	[[Category: Solution structure by nmr]]

OCA at 08:01, 11 November 2020

2020-11-11T08:01:48Z

←Older revision		Revision as of 08:01, 11 November 2020
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-	'''Unreleased structure'''

-	~~The entry 7a58 is ON HOLD until Paper Publication~~	+	==NOE-only solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1==
-		+	<StructureSection load='7a58' size='340' side='right'caption='[[7a58]]' scene=''>
-	~~Authors: Cantini, F., Trindade, I.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A58 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7A58 FirstGlance]. <br>
-	~~Description:~~ NOE-only solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7a58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a58 OCA], [http://pdbe.org/7a58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7a58 RCSB], [http://www.ebi.ac.uk/pdbsum/7a58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7a58 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cantini, F]]	+	__TOC__
-	[[Category: Trindade, I]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cantini F]]
		+	[[Category: Trindade I]]

OCA at 05:02, 2 September 2020

2020-09-02T05:02:53Z

←Older revision		Revision as of 05:02, 2 September 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7a58 is ON HOLD	+	The entry 7a58 is ON HOLD until Paper Publication

	Authors: Cantini, F., Trindade, I.		Authors: Cantini, F., Trindade, I.

-	Description: NOE-only ~~NMR~~ solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1	+	Description: NOE-only solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Trindade, I]]
	[[Category: Cantini, F]]		[[Category: Cantini, F]]
		+	[[Category: Trindade, I]]

OCA: Protected "7a58" [edit=sysop:move=sysop]

2020-08-26T09:19:18Z

Protected "7a58" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7a58 is ON HOLD Authors: Cantini, F., Trindade, I. Description: NOE-only NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomon...

2020-08-26T09:19:16Z

New page: '''Unreleased structure''' The entry 7a58 is ON HOLD Authors: Cantini, F., Trindade, I. Description: NOE-only NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomon...

New page

'''Unreleased structure'''

The entry 7a58 is ON HOLD

Authors: Cantini, F., Trindade, I.

Description: NOE-only NMR solution structure of the Iron-Sulfur protein PioC from Rhodopseudomonas palustris TIE-1
[[Category: Unreleased Structures]]
[[Category: Trindade, I]]
[[Category: Cantini, F]]