7ac4 - Revision history

OCA at 12:06, 1 February 2024

2024-02-01T12:06:57Z

←Older revision		Revision as of 12:06, 1 February 2024
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-	====	+	==Structure of insulin collected by rotation serial crystallography on a COC membrane at a synchrotron source==
-	<StructureSection load='7ac4' size='340' side='right'caption='[[7ac4]]' scene=''>	+	<StructureSection load='7ac4' size='340' side='right'caption='[[7ac4]], [[Resolution\|resolution]] 1.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ac4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AC4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac4 OCA], [https://pdbe.org/7ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.46Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PGR:R-1,2-PROPANEDIOL'>PGR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac4 OCA], [https://pdbe.org/7ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serial data collection has emerged as a major tool for data collection at state-of-the-art light sources, such as microfocus beamlines at synchrotrons and X-ray free-electron lasers. Challenging targets, characterized by small crystal sizes, weak diffraction and stringent dose limits, benefit most from these methods. Here, the use of a thin support made of a polymer-based membrane for performing serial data collection or screening experiments is demonstrated. It is shown that these supports are suitable for a wide range of protein crystals suspended in liquids. The supports have also proved to be applicable to challenging cases such as membrane proteins growing in the sponge phase. The sample-deposition method is simple and robust, as well as flexible and adaptable to a variety of cases. It results in an optimally thin specimen providing low background while maintaining minute amounts of mother liquor around the crystals. The 2 x 2 mm area enables the deposition of up to several microlitres of liquid. Imaging and visualization of the crystals are straightforward on the highly transparent membrane. Thanks to their affordable fabrication, these supports have the potential to become an attractive option for serial experiments at synchrotrons and free-electron lasers.
		+
		+	Versatile microporous polymer-based supports for serial macromolecular crystallography.,Martiel I, Beale JH, Karpik A, Huang CY, Vera L, Olieric N, Wranik M, Tsai CJ, Muhle J, Aurelius O, John J, Hogbom M, Wang M, Marsh M, Padeste C Acta Crystallogr D Struct Biol. 2021 Sep 1;77(Pt 9):1153-1167. doi:, 10.1107/S2059798321007324. Epub 2021 Aug 23. PMID:34473086<ref>PMID:34473086</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ac4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Insulin 3D Structures\|Insulin 3D Structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Sus scrofa]]
		+	[[Category: Huang CY]]
		+	[[Category: Karpik A]]
		+	[[Category: Marsh M]]
		+	[[Category: Martiel I]]
		+	[[Category: Padeste C]]
		+	[[Category: Vera L]]
		+	[[Category: Wang M]]

OCA at 08:58, 29 September 2021

2021-09-29T08:58:09Z

←Older revision		Revision as of 08:58, 29 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7ac4 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ac4' size='340' side='right'caption='[[7ac4]]' scene=''>
-	~~Authors~~: ~~Martiel, I~~.~~, Padeste, C~~.~~, Karpik, A~~.~~, Huang, C~~.Y.~~, Vera, L~~.~~, Wang, M., Marsh, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Structure of insulin collected by rotation serial crystallography on a COC membrane at a synchrotron source~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ac4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ac4 OCA], [https://pdbe.org/7ac4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ac4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ac4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ac4 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Wang~~, ~~M]]~~	+	__TOC__
-	[~~[Category~~: ~~Padeste, C~~]]	+	</StructureSection>
-	[[~~Category~~: ~~Martiel~~, ~~I]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Vera~~, ~~L]]~~	+	[[Category: Z-disk]]
-	[~~[Category~~: ~~Marsh, M]~~]	+
-	[[Category: ~~Karpik, A~~]]	+
-	[[Category: ~~Huang, C.Y~~]]	+

OCA at 03:59, 14 October 2020

2020-10-14T03:59:15Z

←Older revision		Revision as of 03:59, 14 October 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ac4 is ON HOLD	+	The entry 7ac4 is ON HOLD until Paper Publication

	Authors: Martiel, I., Padeste, C., Karpik, A., Huang, C.Y., Vera, L., Wang, M., Marsh, M.		Authors: Martiel, I., Padeste, C., Karpik, A., Huang, C.Y., Vera, L., Wang, M., Marsh, M.
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	[[Category: Wang, M]]		[[Category: Wang, M]]
	[[Category: Padeste, C]]		[[Category: Padeste, C]]
-	[[Category: Karpik, A]]
-	[[Category: Huang, C.Y]]
-	[[Category: Marsh, M]]
	[[Category: Martiel, I]]		[[Category: Martiel, I]]
	[[Category: Vera, L]]		[[Category: Vera, L]]
		+	[[Category: Marsh, M]]
		+	[[Category: Karpik, A]]
		+	[[Category: Huang, C.Y]]

OCA: Protected "7ac4" [edit=sysop:move=sysop]

2020-09-16T06:44:28Z

Protected "7ac4" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 16 September 2020

OCA: New page: '''Unreleased structure''' The entry 7ac4 is ON HOLD Authors: Martiel, I., Padeste, C., Karpik, A., Huang, C.Y., Vera, L., Wang, M., Marsh, M. Description: Structure of insulin collect...

2020-09-16T06:44:27Z

New page: '''Unreleased structure''' The entry 7ac4 is ON HOLD Authors: Martiel, I., Padeste, C., Karpik, A., Huang, C.Y., Vera, L., Wang, M., Marsh, M. Description: Structure of insulin collect...

New page

'''Unreleased structure'''

The entry 7ac4 is ON HOLD

Authors: Martiel, I., Padeste, C., Karpik, A., Huang, C.Y., Vera, L., Wang, M., Marsh, M.

Description: Structure of insulin collected by rotation serial crystallography on a COC membrane at a synchrotron source
[[Category: Unreleased Structures]]
[[Category: Wang, M]]
[[Category: Padeste, C]]
[[Category: Karpik, A]]
[[Category: Huang, C.Y]]
[[Category: Marsh, M]]
[[Category: Martiel, I]]
[[Category: Vera, L]]