OCA at 12:08, 1 February 2024

2024-02-01T12:08:39Z

←Older revision		Revision as of 12:08, 1 February 2024
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	==Crystal structure of active site mutant of SQ Isomerase (YihS-H248A) from Salmonella enterica in complex with sulfofructose (SF)==		==Crystal structure of active site mutant of SQ Isomerase (YihS-H248A) from Salmonella enterica in complex with sulfofructose (SF)==
-	<StructureSection load='7ag4' size='340' side='right'caption='[[7ag4]]' scene=''>	+	<StructureSection load='7ag4' size='340' side='right'caption='[[7ag4]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AG4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ag4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AG4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ag4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ag4 OCA], [https://pdbe.org/7ag4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ag4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ag4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ag4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.13Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RB8:6-deoxy-6-sulfo-D-fructose'>RB8</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ag4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ag4 OCA], [https://pdbe.org/7ag4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ag4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ag4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ag4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SQUS_SALTY SQUS_SALTY] Catalyzes the isomerization of sulfoquinovose (SQ) to 6-deoxy-6-sulfo-D-fructose (SF) (By similarity). In vitro, can also catalyze the interconversion of mannose, fructose and glucose, but has extremely low activity with glucose.[HAMAP-Rule:MF_00998]<ref>PMID:18328504</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The sulfosugar sulfoquinovose (SQ) is produced by essentially all photosynthetic organisms on Earth and is metabolized by bacteria through the process of sulfoglycolysis. The sulfoglycolytic Embden-Meyerhof-Parnas pathway metabolizes SQ to produce dihydroxyacetone phosphate and sulfolactaldehyde and is analogous to the classical Embden-Meyerhof-Parnas glycolysis pathway for the metabolism of glucose-6-phosphate, though the former only provides one C3 fragment to central metabolism, with excretion of the other C3 fragment as dihydroxypropanesulfonate. Here, we report a comprehensive structural and biochemical analysis of the three core steps of sulfoglycolysis catalyzed by SQ isomerase, sulfofructose (SF) kinase, and sulfofructose-1-phosphate (SFP) aldolase. Our data show that despite the superficial similarity of this pathway to glycolysis, the sulfoglycolytic enzymes are specific for SQ metabolites and are not catalytically active on related metabolites from glycolytic pathways. This observation is rationalized by three-dimensional structures of each enzyme, which reveal the presence of conserved sulfonate binding pockets. We show that SQ isomerase acts preferentially on the beta-anomer of SQ and reversibly produces both SF and sulforhamnose (SR), a previously unknown sugar that acts as a derepressor for the transcriptional repressor CsqR that regulates SQ-utilization. We also demonstrate that SF kinase is a key regulatory enzyme for the pathway that experiences complex modulation by the metabolites SQ, SLA, AMP, ADP, ATP, F6P, FBP, PEP, DHAP, and citrate, and we show that SFP aldolase reversibly synthesizes SFP. This body of work provides fresh insights into the mechanism, specificity, and regulation of sulfoglycolysis and has important implications for understanding how this biochemistry interfaces with central metabolism in prokaryotes to process this major repository of biogeochemical sulfur.
		+
		+	Molecular Basis of Sulfosugar Selectivity in Sulfoglycolysis.,Sharma M, Abayakoon P, Epa R, Jin Y, Lingford JP, Shimada T, Nakano M, Mui JW, Ishihama A, Goddard-Borger ED, Davies GJ, Williams SJ ACS Cent Sci. 2021 Mar 24;7(3):476-487. doi: 10.1021/acscentsci.0c01285. Epub, 2021 Feb 23. PMID:33791429<ref>PMID:33791429</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ag4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
	[[Category: Davies GJ]]		[[Category: Davies GJ]]
	[[Category: Sharma M]]		[[Category: Sharma M]]

OCA at 06:45, 14 April 2021

2021-04-14T06:45:09Z

←Older revision		Revision as of 06:45, 14 April 2021
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-	'''Unreleased structure'''

-	~~The entry 7ag4 is ON HOLD until Sep 21 2022~~	+	==Crystal structure of active site mutant of SQ Isomerase (YihS-H248A) from Salmonella enterica in complex with sulfofructose (SF)==
-		+	<StructureSection load='7ag4' size='340' side='right'caption='[[7ag4]]' scene=''>
-	~~Authors: Sharma, M., Davies, G.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AG4 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of active site mutant of SQ Isomerase (YihS-H248A) from Salmonella enterica in complex with sulfofructose (SF)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ag4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ag4 OCA], [https://pdbe.org/7ag4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ag4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ag4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ag4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Sharma, M~~]]	+	__TOC__
-	[[Category: ~~Davies, G.J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Davies GJ]]
		+	[[Category: Sharma M]]

OCA: Protected "7ag4" [edit=sysop:move=sysop]

2020-10-28T18:51:23Z

Protected "7ag4" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:51, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7ag4 is ON HOLD until Sep 21 2022 Authors: Sharma, M., Davies, G.J. Description: Crystal structure of active site mutant of SQ Isomerase (YihS-H248...

2020-10-28T18:51:22Z

New page: '''Unreleased structure''' The entry 7ag4 is ON HOLD until Sep 21 2022 Authors: Sharma, M., Davies, G.J. Description: Crystal structure of active site mutant of SQ Isomerase (YihS-H248...

New page

'''Unreleased structure'''

The entry 7ag4 is ON HOLD until Sep 21 2022

Authors: Sharma, M., Davies, G.J.

Description: Crystal structure of active site mutant of SQ Isomerase (YihS-H248A) from Salmonella enterica in complex with sulfofructose (SF)
[[Category: Unreleased Structures]]
[[Category: Sharma, M]]
[[Category: Davies, G.J]]

7ag4 - Revision history

OCA at 12:08, 1 February 2024

OCA at 06:45, 14 April 2021

OCA: Protected "7ag4" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ag4 is ON HOLD until Sep 21 2022 Authors: Sharma, M., Davies, G.J. Description: Crystal structure of active site mutant of SQ Isomerase (YihS-H248...